Glycine, N-methyl-, N-coco acyl derivs., sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A defined amount of the test substance was weighed into a measuring flask and the total volume was filled up with dilution water. The test bottles were conditioned about 15 min before the test was started.
- Differential loading: yes
- Controls: yes, dilution water served as negative control

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Length at study initiation: 2.0 cm (average length)

ACCLIMATION
- Acclimation period: at least 14 d before test
- Acclimation conditions: day/night change, circulation filters, 21 °C
- Feeding frequency during acclimation: daily

FEEDING DURING TEST
- Frequency: no feeding during test

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

0 h: 19.5 (control), 19.4 - 19.6 °C (test solutions)
96 h: 20.2 (control), 19.6 - 19.9 °C (test solutions)

pH:

At 0 h: 7.9 (control), 8.0 (test solutions)
At 96 h: 7.9 (control), 7.6 - 7.9 (test solutions)

Dissolved oxygen:

At 0 h: 7.0 mg/L (control), 6.8 - 7.8 mg/L (test solutions)
At 96 h: 6.0 mg/L (control), 5.5 - 6.9 mg/L (test solutions), corresponding to 66% of the air saturation

Nominal and measured concentrations:

nominal: control, 2.5, 5, 10, 25, 50 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Material, size, headspace, fill volume: Fill volume: 7 L
- Aeration: yes, continuously
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chlorine-free drinking water; Hardness: 37.4 mg/L Ca
- Metals: Copper content: < 0.01 mg/L
- Chlorine: none
- Culture medium different from test medium: no
- Intervals of water quality measurement: 24 h. PH values and O2 concentration were measured at 0, 24, 48, 72 and 96 h

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h light/dark cycle
- Light intensity: indirect day-similar light

EFFECT PARAMETERS MEASURED:
The fish were inspected at 24, 48, 72 and 96 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: no

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: 0%
- Any observations that might cause a difference between measured and nominal values: no

Reported statistics and error estimates:

The statistical evaluation was based on a Probit analysis. The calculation of the Probit values with the date after 24 to 96 h was performed with the computer program "Dosis 50". In this program the experimentally determined values ranging from 1 - 99% can be used for the calculation of the Probit values and the evaluation of the effective concentrations. For the experimentally determined percentages of 5 and 100% no probit values are defined. Therefore, the values for 1% and 99% were used to calculate the probit values of these percentages, respectively.

Sublethal observations / clinical signs:

The highest tested concentration of test substance producing no mortality under test conditions after 96 h was 2.5 mg/L.

The lowest tested concentration of test substance producing 100% mortality under test conditions after 96 h was 50 mg/L.

Table 1. Mortality in 24 h intervals.

Concentration of test substance [mg/L]	Mortality [%] after 24 h	Mortality [%] after 48 h	Mortality [%] after 72 h	Mortality [%] after 96 h
100	100	100	100	100
50	86	100	100	100
25	29	57	71	71
10	14	14	29	29
5.0	0	0	14	14
2.5	0	0	0	0
control	0	0	0	0

 

Description of key information

LC50 (96 h) = 14.4 mg/L (nominal, Danio rerio, OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

14.4 mg/L

Additional information

One GLP-study according to OECD 203 investigating the short-term toxicity of Glycine, N-methyl-, N-coco acyl derivatives, sodium salts (CAS 61791-59-1) to freshwater fish is available. The test organism Danio rerio was exposed to six nominal test concentrations ranging from 2.5 - 100 mg/L (2.5, 5, 10, 25, 50 and 100 mg/L) in controlled conditions for 96 h. No analytical measurement was conducted. Mortality was recorded in daily intervals and was compared to the negative control at test termination. After 96 h mortality was observed at concentrations ≥ 5.0 mg/L. The derived nominal LC50 value after 96 h was 14.4 mg/L.