Synthetic resin, a reaction product of 4,4'-bis(N-maleimido)diphenylmethane with 3-aminobenzoic acid hydrazide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Rationale was chosen based on high deviations between replicates and inconsistent COD/BOD values

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Version / remarks:

12 May 1981

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-(methylenedi-p-phenylene)bismaleimide

EC Number:

237-163-4

EC Name:

1,1'-(methylenedi-p-phenylene)bismaleimide

Cas Number:

13676-54-5

Molecular formula:

C21H14N2O4

IUPAC Name:

1,1'-[methylenedi(4,1-phenylene)]di(1H-pyrrole-2,5-dione)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Domestic waste-water plant (ARA Sissach/Switzerland)
- Pretreatment: The sludge used for this study was washed three times with tap water and an amount corresponding to of 4 g dry material per litre was mixed with Soerensen buffer solution (pH 7) and then aerated prior to incubation. About 30 minutes after stopping the aeration of the sludge, approximately 1/3 of the whole volume of the supernatant was removed. An equal volume of 0.1 % glucose, peptones and monopotassium phosphate was added to the remaining sludge, and aerated again. The pH of the sludge was adjusted to 7 ± 1.
- Concentration of sludge: 25 mg for the treated and the untreated flasks and 7.5 mg for the ANILINE treated flasks

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation 1

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation 2

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: according to OECD guideline 302C
- Test temperature: 22-31°C, was controlled daily during the study. The temperature was slightly above the temperature recommended by the guidelines. However, it can be stated that these transitory temperature differences had no effect on the integrity of the test.
- pH: Was monitored for all incubation flasks at the end of the incubation period.
- pH adjusted: yes, in the final test medium and the sludge
- Aeration of dilution water: At preparation of the flasks
- Continuous darkness: yes by means of brown glass bottles

TEST SYSTEM
- Culturing apparatus: BSB 1000 and BSB 601 apparatus (Wissenschaftliche-Technische Werkstätten GmbH, Weilheim, FRG)
- Number of culture flasks/concentration: 3 for the treated flasks and one each for the negative controls.
Flask 9: 250 ml bidistilled water and 7.5 mg test article
Flask 10+11+12: 250 ml final test medium, 7.5 mg test article (pH 7.0) and 25 mg inoculum.
Flask 13+14+15: 250 ml final test medium, 25 mg ANILINE and 7.5 mg inoculum.
Flask 16: 250 ml final test medium and 25 mg inoculum.
- Measuring equipment: carbon analyser (Technicon Autoanalyser II)
- Details of trap for CO2 and volatile organics if used: The CO2 was adsorbed by NaOH and the resulting drop in pressure was read on a manometer. The test vessel was opened and the NaOH replaced three times per week, except between day 8 and day 13.

SAMPLING
- Sampling frequency: daily

CONTROL AND BLANK SYSTEM
- Inoculum blank: final test medium + inoculum
- Abiotic sterile control: bidistilled water + test article

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

37.8

Sampling time:

28 d

Details on results:

A degradation of 0 - 55.3 % was observed when estimated using the DOC-values. Furthermore, it has to be stated that a degradation in the same order of magnitude was observed in the flask without inoculum, allowing the assumption that the test article was not only degraded by microorganisms. It cannot be excluded that the insolubility of COMPIMIDE MDAB in aqueous solution may prevent the test article to be optimally degraded by aquatic microorganisms. Therefore, the test article may be degradable to a higher amount as indicated by the value in the present study.

BOD5 / COD results

Results with reference substance:

The standard compound, ANILINE, was readily degraded after 14 and 28 days by 64.0% and 64.3% (BOD/ThOD), respectively.

Any other information on results incl. tables

Table 1: Biochemical Oxygen Demand (BOD) of the test item and ANILINE

Flask No.	Test article concentration (mg/mL)	Cumulative BOD-values after x-days (mgO2/100 mg test article)
		1	2	3	4	5	6	7
9^1	7.5	58.3	58.3	58.3	58.3	58.3	58.3	58.3
10	7.5	50	50	50	50	50	50	66.7
11	7.5	83.3	83.3	83.3	83.3	100	100	133.3
12	7.5	0	0	0	0	0	0	0
13	25 (ANILINE)	0	0	105	150	150	150	150
14	25 (ANILINE)	0	0	105	135	135	135	135
15	25 (ANILINE)	0	0	120	175	177.5	177.5	177.5
Flask No.	Test article concentration (mg/mL)	Cumulative BOD-values after x-days (mgO2/100 mg test article)
		8	9	10	11	12	13	14
9^1	7.5	58.3	75	75	75	75	75	75
10	7.5	66.7	66.7	66.7	66.7	66.7	66.7	66.7
11	7.5	133.3	133.3	150	150	200	200	225
12	7.5	0	0	0	0	0	0	0
13	25 (ANILINE)	150	150	150	150	150	150	150
14	25 (ANILINE)	135	135	135	135	135	135	135
15	25 (ANILINE)	177.5	177.5	177.5	177.5	177.5	177.5	177.5
Flask No.	Test article concentration (mg/mL)	Cumulative BOD-values after x-days (mgO2/100 mg test article)
		15	16	17	18	19	20	21
9^1	7.5	75	83.3	83 .3	83.3	83.3	83.3	100
10	7.5	66.7	66.7	66.7	66.7	83.3	83.3	83.3
11	7.5	225	225	258.3	258.3	300	300	325
12	7.5	0	0	8.3	8.3	41.7	41.7	41.7
13	25 (ANILINE)	150	150	150	150	150	150	150
14	25 (ANILINE)	135	135	137.5	137.5	137.5	137.5	137.5
15	25 (ANILINE)	177.5	177.5	177.5	177.5	177.5	177.5	177.5
Flask No.	Test article concentration (mg/mL)	Cumulative BOD-values after x-days (mgO2/100 mg test article)
		22	23	24	25	26	27	28
9^1	7.5	100	100	100	100	100	100	100
10	7.5	83.3	83.3	83.3	83.3	83.3	83.3	83.3
11	7.5	325	375	375	375	525	533.3	533.3
12	7.5	41.7	41.7	41.7	41.7	58.3	58.3	58.3
13	25 (ANILINE)	150	150	150	150	150	150	150
14	25 (ANILINE)	137.5	137.5	137.5	137.5	137.5	137.5	137.5
15	25 (ANILINE)	177.5	177.5	177.5	177.5	177.5	177.5	177.5

¹: Test article without inoculum

Table 2: Degradation of the test item and ANILINE (BOD/ThOD)

Flask No.	Test article concentration (mg/ 250mL)	Percentage BD after x days
		1	2	3	4	5	6	7
9^1	7.5	31.1	31.1	31.1	31.1	31.1	31.1	31.1
10	7.5	26.7	26.7	26.7	26.7	26.7	26.7	35.6
11^2	7.5	44.4	44.4	44.4	44.4	53.3	53.3	71.1
12	7.5	0	0	0	0	0	0	0
MEAN^3		13.4	13.4	13.4	13.4	13.4	13.4	17.8
13	25 (ANILINE)	0	0	43.6	62.2	62.2	62.2	62.2
14	25 (ANILINE)	0	0	43.6	56	56	56	56
15	25 (ANILINE)	0	0	49.8	72.6	73.7	73.7	73.7
MEAN		0	0	45.7	63.6	64	64	64
Flask No.	Test article concentration (mg/mL)	Percentage BD after x days
		8	9	10	11	12	13	14
9^1	7.5	31.3	40	40	40	40	40	40
10	7.5	35.6	35.6	35.6	35.6	35.6	35.6	35.6
11^2	7.5	71.1	71.1	80	80	106.7	106.7	120
12	7.5	0	0	0	0	0	0	0
MEAN^3		17.8	17.8	17.8	17.8	17.8	17.8	17.8
13	25 (ANILINE)	62.2	62.2	62.2	62.2	62.2	62.2	62.2
14	25 (ANILINE)	56	56	56	56	56	56	56
15	25 (ANILINE)	73.7	73.7	73.7	73.7	73.7	73.7	73.7
MEAN		64	64	64	64	64	64	64
Flask No.	Test article concentration (mg/mL)	Percentage BD after x days
		15	16	17	18	19	20	21
9^1	7.5	40	44.4	44.4	44.4	4.4	44.4	53.3
10	7.5	35.6	35.6	35.6	35.6	44.4	44.4	44.4
11^2	7.5	120	120	137.8	1378	160	160	173.3
12	7.5	0	0	4.4	4.4	22.2	22.2	22.2
MEAN^3		17.8	17.8	20	20	33.3	33.3	33.3
13	25 (ANILINE)	62.2	62.2	62.2	62.2	62.2	62.2	62.2
14	25 (ANILINE)	56	56	57.1	57.1	57.1	57.1	57.1
15	25 (ANILINE)	73.7	73.7	73.7	73.7	73.7	73.7	73.7
MEAN		64	64	64.3	64.3	64.3	64.3	64.3
Flask No.	Test article concentration (mg/mL)	Percentage BD after x days
		22	23	24	25	26	27	28
9^1	7.5	53.3	53.3	53.3	53.3	53.3	53.3	53.3
10	7.5	44.4	44.4	44.4	44.4	44.4	44.4	44.4
11^2	7.5	173.3	200	200	200	280	284.4	284.4
12	7.5	22.2	22.2	22.2	22.2	31.1	31.1	31.1
MEAN^3		33.3	33.3	33.3	33.3	37.8	37.8	37.8
13	25 (ANILINE)	62.2	62.2	62.2	62.2	62.2	62.2	62.2
14	25 (ANILINE)	57.1	57.1	57.1	57.1	57.1	57.1	57.1
15	25 (ANILINE)	73.7	73.7	73.7	73.7	73.7	73.7	73.7
MEAN		64.3	64.3	64.3	64.3	64.3	64.3	64.3

¹: Test article without inoculum

²: Qutlier value

³: Mean of flask 10 and 12

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

In the present study conducted according to OECD guideline 302C (adopted 12 May 1981) activated sewage sludge was incubated with 30 mg of the test item for 28 days. Measured BOD/ThOD values relvealed a biodegradation of 37.8% within 28 days, whereas the claculated DOC-values were between 0 and 53% degradation.