(+)-neomenthol

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical (+)-Neomenthol (2216 -52 -6).The studies are as mentioned below:

1. The short term toxicity study of test material was evaluated for aquatic invertebrate Biomphalaria alexandrina snails .

 The snails were collected from fresh water ponds (untreated with molluscicides). Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l of dechlorinated tap water, fed on dry lettuce leaves and oxygenated for 1 h daily, for 1 week prior to experimentation.The median lethal effect concentration (LC50) for 24 h was observed to be 101.59 mg/l (with 95% confidence limits 89.45 – 115.4).

2.Short term toxicity of test material was evaluated for test material Ten neonates less than 24 hr old Daphnids were used for the test  . Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90.

 Two replicates were used in each test. Daphnia were gently touched with a pastier pipet at 24 and 48 hr.If the daphnia could not swim actively for 15 s, immobilization (mortality) was recorded. The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis. The effect concentration of test chemical (EC50) for 50% immobilization of Daphnia magna was observed to be 276.0 mg/l.

On the basis of above effect concentration it can be considered that the test material has no toxic effect on aquatic invertebrates and cannot be classified as per CLP criteria.

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical (+)-Neomenthol (2216 -50 -6).The studies are as mentioned below:

1.Toxicity of test material on aquatic algae and cyanobacteria was evaluated on S. capricornutum.

Stock cultures of algae were maintained on 1.5% Difco-Bacto agar slants. Test algae were grown in a semiflow-through culture apparatus on T82MV(7) and taken during log phase growth for inoculation into the test flasks. 500 ml Erlenmeyer flasks with ground glass stoppers were used as test chambers. 100 ml of media was placed in each test chamber and inoculated with approximately 4.0 X 104 algal cells per milliliter. The algae were placed in an incubator . Using a Newbauer Counting Chamber, cell densities were determined every 24 hr for 5 consecutive days. The effect concentartion (IC50) after 96 h for the test material was observed to be >100 mg/l.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.

2. Toxicity of test material was evaluted for Scenedesmus subspicatus (Green Algae ) in a static cocndition for 7 days. The effect concentration (EC0) of test material on Scenedesmus subspicatus (Green Algae ) after 7 days was observed to be 263 mg/l. Based on the above test concentartion it can be considered that the test material is non toxic to aquatic algae and hence, can be considered to be not classified as per CLP classification.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.

 

Additional information

Short term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical (+)-Neomenthol (2216 -52 -6).The studies are as mentioned below:

1. The short term toxicity study of test material was evaluated for aquatic invertebrate Biomphalaria alexandrina snails .

 The snails were collected from fresh water ponds (untreated with molluscicides). Specimens were acclimated to laboratory conditions in a glass aquarium containing 5 l of dechlorinated tap water, fed on dry lettuce leaves and oxygenated for 1 h daily, for 1 week prior to experimentation.The median lethal effect concentration (LC50) for 24 h was observed to be 101.59 mg/l (with 95% confidence limits 89.45 – 115.4).

2.Short term toxicity of test material was evaluated for test material Ten neonates less than 24 hr old Daphnids were used for the test  . Daphnid stock cultures were fed a mixture of A. falcatus, S. capricornutum, and Chlamydomonas reinhardi 90.

 Two replicates were used in each test. Daphnia were gently touched with a pastier pipet at 24 and 48 hr.If the daphnia could not swim actively for 15 s, immobilization (mortality) was recorded. The effective concentrations at which 50% of the organisms are immobilized (EC50) were computed using the probit analysis. The effect concentration of test chemical (EC50) for 50% immobilization of Daphnia magna was observed to be 276.0 mg/l.

On the basis of above effect concentration it can be considered that the test material has no toxic effect on aquatic invertebrates and cannot be classified as per CLP criteria.

 

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical (+)-Neomenthol (2216 -50 -6).The studies are as mentioned below:

1.Toxicity of test material on aquatic algae and cyanobacteria was evaluated on S. capricornutum.

Stock cultures of algae were maintained on 1.5% Difco-Bacto agar slants. Test algae were grown in a semiflow-through culture apparatus on T82MV(7) and taken during log phase growth for inoculation into the test flasks. 500 ml Erlenmeyer flasks with ground glass stoppers were used as test chambers. 100 ml of media was placed in each test chamber and inoculated with approximately 4.0 X 104 algal cells per milliliter. The algae were placed in an incubator . Using a Newbauer Counting Chamber, cell densities were determined every 24 hr for 5 consecutive days. The effect concentartion (IC50) after 96 h for the test material was observed to be >100 mg/l.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.

2. Toxicity of test material was evaluted for Scenedesmus subspicatus (Green Algae ) in a static cocndition for 7 days. The effect concentration (EC0) of test material on Scenedesmus subspicatus (Green Algae ) after 7 days was observed to be 263 mg/l. Based on the above test concentartion it can be considered that the test material is non toxic to aquatic algae and hence, can be considered to be not classified as per CLP classification.

Based on the above effect concentration it can be considered that ttest material has no toxic effect on algae . Hence , the test material is considered to be not classified as per CLP classification.