Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 218-159-1 | CAS number: 2057-49-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 November to 13 December 2017
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 2009
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- The test item is listed as 4-PPP (CAS # 2057-49-0) and is a clear amber colored liquid. The test substance was obtained from the Sponsor, Vertellus Holdings LLC and stored at room temperature in the dark.
- Analytical monitoring:
- yes
- Remarks:
- Gas Chromatography
- Vehicle:
- no
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test was carried out using the green alga Pseudokirchneriella subcapitata. Liquid cultures of Pseudokirchneriella subcapitata were obtained from the Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland. Master cultures were maintained in the laboratory by the periodic replenishment of culture medium. The master cultures were maintained in the laboratory under constant agitation by orbital shaker (100 to 150 rpm) and constant illumination at 24 ±1 °C.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 °C ± 1 °C
- pH:
- 7.1 to 8.1
- Nominal and measured concentrations:
- The test item was evaluated at nominal concentrations of 0.10 mg/L, 0.32 mg/L, 1.0 mg/L, 3.2 mg/L and 100 mg/L) (three replicate flasks per concentration). Results were based on the 0-h mean measured test concentrations only. These were determined to be 0.096, 0.23, 0.77, 2.1 and 7.4 mg/L (66% to 96% of the nominal test concentrations).
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate was the reference item at concentrations of 0.25 mg/L, 0.50 mg/L, 1.0mg/L, 2.0 mg/L and 4.0 mg/L. The results from the positive control with potassium dichromate were within the normal ranges for this reference item.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- Based on the 0-hour mean measured test concentration
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.77 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- Based on the 0-hour mean measured test concentration
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- Based on the 0-hour mean measured test concentration
- Results with reference substance (positive control):
- The results from the positive control with potassium dichromate were within the normal ranges for this reference item.
- Validity criteria fulfilled:
- yes
- Remarks:
- Cell concentrations of control increased by a factor of 191 after 72 h (protocol ≥ factor of 16); mean of coeffs of var.for control growth rates 25% (protocol ≤ 35%); coeff. of var.of avg. sp.growth rate in replicate control cultures 3% (protocol ≤ 7%).
- Conclusions:
- The test substance caused a reduction of growth in a series of algal cultures (test units) exposed to the test item for 72 h. Results were based on the 0-h mean measured test concentrations. The 72-h EC50 was 1.8 mg/L and the 72-h LOEC was 2.1 mg/L as determined by comparison of areas in growth curves. The 72-h NOEC was 0.77 mg/L as determined by comparison of areas in growth curves.
Reference
Description of key information
The key study was a static acute toxicity test on the effects of the substance on the freshwater unicellular green alga Pseudokirchneriella subcapitata following OECD Guideline 201. The test substance caused a reduction of growth in a series of algal cultures (test units) exposed to the test item for 72 h. Results were based on the 0-h mean measured test concentrations. The 72-h EC50 was 1.8 mg/L and the 72-h LOEC was 2.1 mg/L as determined by comparison of areas in growth curves. The 72-h NOEC was 0.77 mg/L as determined by comparison of areas in growth curves.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.8 mg/L
- EC10 or NOEC for freshwater algae:
- 0.77 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
