Registration Dossier

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Acute aquatic hazard: not classified, according to the CLP Regulation (EC) No 1272/2008

Long-term aquatic hazard: Aquatic Chronic 3, H412, according to the CLP Regulation (EC) No 1272/2008

Additional information

Adequate chronic toxicity data are not available for Acid Blue 225, therefore the aquatic toxicity assessment and classification are based mainly on the short-term toxicity data available on the substance as such and the data the Acid Blue 225_constituent 1. The approach can be considered as appropriate and suitable for the purpose (details are reported into the IUCLID section 13).

Although Acid Blue 225 is not readily biodegradable, it is expected to have a low log Pow lower than 4 and low potential for bioaccumulation on the basis of the chemical structure and characteristics.

According to the CLP Regulation (EC) No 1272/2008, acute aquatic toxicity is normally determined using a fish 96-hour LC50, a crustacean species 48-hour EC50 and/or an algal species 72- or 96-hour EC50; the mentioned species cover a range of trophic levels and taxa and are considered as surrogate for all aquatic organisms. Data on other species (e.g. Lemna spp.) shall also be considered if the test methodology is suitable.

In the case of Acid Blue 225 and Acid Blue 225_constituent 1, data are available for fish (Poecilia reticulata), invertebrates (Daphnia magna) and lemna (Lemna minor).

No L(E)C50 value was recorded at concentration equal/lower than 1 mg/l, thus the classification criteria for acute (short-term) aquatic hazard (category 1) of the CLP Regulation (EC) No 1272/2008 are not met.

Regarding the evaluation of the long-term aquatic hazard, the outcomes of the tests performed on the three trophic levels suggest that fish is the most sensitive species, thus the classification should be based on the short-term toxicity to this organism.

The available studies on short-term toxicity to fish fixed the short-term effect level between 10 and 100 mg/l, thus the substance meets the criteria to be classified for long-term aquatic hazard, category 3 (i.e. Aquatic Chronic 3, H412), of the CLP Regulation (EC) No 1272/2008.

Categories Display