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EC number: 701-394-3 | CAS number: 1782069-81-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 July 1994 to 18 August 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- not specified
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Fresh activated sludge was collected from a biological wastewater treatment plant treating predominantly domestic sewage (City of Geneva, Aïre) in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until used on the same day.
- Storage conditions: Sludge was kept aerobic.
- Storage length: Sludge was used on the same day as collection.
- Concentration of sludge: 2.988 g/L dry weight
- Water filtered: no - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral components for Solutions A (8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4x2H2O, 0.5 g NH4Cl), B (27.5 g CaCl2), C (22.5 g MgSO4x7H2O) and D (0.25 g FeCl3x6H2O, one drop concentrated HCl) were dissolved in water and made up to 1 L each. The mineral medium was prepared by mixing 50 mL of solution A and 2000 mL deionised water, adding 5 mL of each of the solutions B, C and D and making up to 5 L with deionised water.
- Test temperature: 20°C
- pH: 7.36 to 8.54
- pH adjusted: Yes, to 7.4 ± 0.2 with phosphoric acid or potassium hydroxide
- Suspended solids concentration: 30 mg/L dry weight
TEST SYSTEM
- Culturing apparatus: SAPROMAT test flask
- Number of culture flasks/concentration: 2
- Measuring equipment: SAPROMAT D 12
- Test performed in open system: No, closed system
- Details of trap for CO2 and volatile organics if used: Evolved carbon dioxide was absorbed in soda lime pellets.
SAMPLING
- Sampling frequency: The oxygen consumption of each flask was recorded daily and the pH of each flask was measured at the start and end of the test period.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
STATISTICAL METHODS: No statistical analysis was performed. Oxygen uptakes were corrected by deducing the basic oxygen uptake of sludge and to account for the differences between actual and nominal concentrations of the test substance. - Reference substance:
- aniline
- Preliminary study:
- Not performed.
- Test performance:
- The test is considered valid as the degradation of aniline was > 40% after 7 days and > 65% after 14 days.
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 5 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- -1
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- -1
- Sampling time:
- 10 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- -2
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- -2
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- -2
- Sampling time:
- 28 d
- Details on results:
- The test substance is not readily biodegradable, possibly due to its physical state (solid) and the low water solubility.
- Key result
- Parameter:
- ThOD
- Value:
- 2.89 mg O2/g test mat.
- Results with reference substance:
- ThOD of aniline: 2.41 mg O2/g
- Validity criteria fulfilled:
- yes
- Remarks:
- The degradation of aniline was > 40% after 7 days and > 65% after 14 days.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance is not readily biodegradable, as no degradation was observed after 28 days.
- Executive summary:
The biodegradability of the test substance was tested according to OECD Guideline 301F. A nominal concentration of 100 mg/L test substance was incubated with activated sludge for 28 days under aerobic conditions, alongside an inoculum blank and a toxicity control. Oxygen consumption was measured electrolytically daily. The test substance is not readily biodegradable, as no degradation was observed after 28 days. This result may be due to the physical state (solid) and low water solubility of the test substance. This study is considered to be reliable without restriction (Klimisch 1).
Reference
Table 1. Biodegradability results
| Parameter | Flask | Biological Oxygen Demand (BOD, mg O2/L, adjusted to nominal concentrations) | |||||
| Day 5 | Day 7 | Day 10 | Day 14 | Day 21 | Day 28 | ||
| BOD Sludge | 1 | 23.0 | 25.0 | 26.0 | 29.0 | 33.0 | 33.0 |
| 2 | 23.0 | 25.0 | 26.0 | 29.0 | 33.0 | 33.0 | |
| Mean | 23.0 | 25.0 | 26.0 | 29.0 | 33.0 | 33.0 | |
| BOD Test substance | 1 | 20.0 | 22.0 | 23.0 | 23.1 | 24.1 | 24.1 |
| 2 | 24.0 | 24.0 | 26.0 | 26.1 | 28.1 | 28.1 | |
| 1C | -3.0 | -3.0 | -3.0 | -5.9 | -8.9 | -8.9 | |
| 2C | 1.0 | -1.0 | 0.0 | -2.9 | -4.9 | -4.9 | |
| % degradation | 1 | -1 | -1 | -1 | -2 | -3 | -3 |
| 2 | 0 | 0 | 0 | -1 | -2 | -2 | |
| mean | 0 | -1 | -1 | -2 | -2 | -2 | |
C: BOD results were corrected for the mean oxygen uptake of sludge
Description of key information
The test substance is not readily biodegradable, as no degradation was observed after 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The biodegradability of the test substance was tested according to OECD Guideline 301F (1994). A nominal concentration of 100 mg/L test substance was incubated with activated sludge for 28 days under aerobic conditions, alongside an inoculum blank and a toxicity control. Oxygen consumption was measured electrolytically daily. The test substance is not readily biodegradable, as no degradation was observed after 28 days. This result may be due to the physical state (solid) and low water solubility of the test substance. This study is considered to be reliable without restriction (Klimisch 1).
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