Sodium 3-sulphonatopropyl acrylate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 Aug - 14 Sep 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP - Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Remarks:

The Department of health of the Government of the United Kingdom

Test material

Test material information

Constituent 1

Reference substance name:

Potassium 3-sulphonatopropyl acrylate

EC Number:

250-465-0

EC Name:

Potassium 3-sulphonatopropyl acrylate

Cas Number:

31098-20-1

Molecular formula:

C6H10O5S.K

IUPAC Name:

potassium 3-(acryloyloxy)propane-1-sulfonate

Details on test material:

- Name of test material (as cited in study report): SPA (sulfopropyl acrylate)
- Physical state: powder
- Analytical purity: no data
- Lot/batch No.: 0200014/001
- Storage condition of test material: at room temperature in the dark

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Final effluent of Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, United Kingdom, obtained on 17 Aug 2001
- Pretreatment: The effluent was filtered through coarse filter paper, while approx. the first 200 mL were discarded. The filtrate was maintained on continuous aeration in a temperature controlled room at 21 °C.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: 100 mg test substance were directly added to the culture medium (according to OECD 301D) and dissolved with the aid of ultrasonication (5 min). The volume was adjusted to 100 mL to give a 1000 mg/L stock solution which was inverted several times to ensure adequate mixing and homogeneity. An aliquot of 30 mL was dispersed into the final volume of 6 L to give a concentration of 5 mg/L. 1 drop inoculum per litre was added to each test flask.
- Test temperature: 20 °C
- pH adjusted: no

TEST SYSTEM
- Culturing apparatus: 250 - 300 mL darkened glass bottles (BOD bottles), stoppered
- Number of culture flasks/concentration: 2
- Measuring equipment: Yellow Springs oxygen meter and BOD Probe

SAMPLING
- Sampling frequency: after 0. 3, 6, 9, 12, 15, 18, 21, 24 and 28 d

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: -
- Toxicity control: yes, containing 5 mg/L test substance and 1.5 mg/L reference substance

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

3 mg/L

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

28

Sampling time:

28 d

Details on results:

The test substance was degraded by 28% within 28 d and thus, can not be considered to be readily biodegradable.
The toxicity control was degraded by 27% within 15 d. Since the biodegradation was > 25 % the test substance is not considered to be inhibitory to the inoculum.

BOD5 / COD results

Results with reference substance:

The reference substance is degraded by 74% within the 14-day window, confirming the inoculum and the test set-up to be appropriate.

Any other information on results incl. tables

Table 1: Biodegradation of the test substance, control, reference und toxic control after 28 d

days	mean biodegradation [%]
	test substance	inoculum blank	reference substance	toxicity control
3	5	0.450	41	10
6	7	0.575	56	10
9	10	0.625	57	11
12	29	0.675	65	29
15	28	0.825	65	27
18	27	0.850	73	28
21	27	0.850	74	29
24	27	0.950	75	29
28	28	1.050	74	29

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable