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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 Aug - 17 Aug 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP - Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Remarks:
The Department of Health of the Government of the United Kingdom

Test material

Constituent 1
Chemical structure
Reference substance name:
Potassium 3-sulphonatopropyl acrylate
EC Number:
250-465-0
EC Name:
Potassium 3-sulphonatopropyl acrylate
Cas Number:
31098-20-1
Molecular formula:
C6H10O5S.K
IUPAC Name:
potassium 3-(acryloyloxy)propane-1-sulfonate
Details on test material:
- Name of test material (as cited in study report): SPA (sulfopropyl acrylate)
- Physical state: powder
- Analytical purity: no data
- Lot/batch No.: 0200014/001
- Storage condition of test material: at room temperature in the dark

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: Pooled replicate water samples were taken from the control and the 100 mg/L test group at 0 and 48 h for quantitative analysis.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 200 mg test material was directly added to reconstituted water (ISO medium) and the volume adjusted to give a test concentration of 100 mg/L. The solution was inverted several times to ensure adequate mixing and homogeneity.
- Evidence of undissolved material: The test preparations were observed to be clear, colourless solutions throughout the study.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age at study initiation: < 24 h
- Feeding during test: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
21 °C, measured at test start and after 24 and 48 h in every test vessel and the control.
pH:
7.9 - 8.0, measured at test start and after 48 h in every test vessel and the control.
Dissolved oxygen:
8.1 - 8.4 mg O2/L, (91 - 94% of saturation), measured at test start and after 48 h in every test vessel and the control.
Nominal and measured concentrations:
nominal: 0 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel
- Type: Test vessels were covered with glass lids to reduce evaporation.
- Material, size, fill volume: 250 mL glass jar containing approx. 200 mL test solution
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (ISO medium) as stated in OECD 202

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark with 20 min dawn and dusk transition periods

EFFECT PARAMETERS MEASURED: immobilisation and adverse reactions after 24 and 48 h

TEST CONCENTRATIONS
- Range finding study:
- Test concentrations: 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilisation was observed up to a concentration of 100 mg/L.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: No adverse reactions were observed.
- Mortality of control: no

Any other information on results incl. tables

Analysis of the test preparations sampled at test start and after 48 h showed the measured test concentrations to be near nominal (102 - 105%).

Applicant's summary and conclusion