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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP - Guideline study with acceptable restrictions Data on pH and temperature of the test solutions and reference substance (concentrations, EC50) are missing. No analytics were conducted.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Potassium 3-sulphonatopropyl acrylate
EC Number:
250-465-0
EC Name:
Potassium 3-sulphonatopropyl acrylate
Cas Number:
31098-20-1
Molecular formula:
C6H10O5S.K
IUPAC Name:
potassium 3-(acryloyloxy)propane-1-sulfonate
Details on test material:
- Name of test material (as cited in study report): Kalium-3-sulfonatopropylacrylat
- Physical state: solid
- Analytical purity: no data
- Lot/batch No.: ss 32799-465
- Storage condition of test material: in a refrigerator at 5 - 10°C

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared one day before test start by adding 125 mg test substance to 800 mL destilled water and 24 h treatment with a magnetic stirrer. Afterwards 200 mL distilled water were added. Each test concentration is 200 mL, consisting of stock solution, 20 mL nutrient solution, 20 mL inoculum and bidestilled water if dilution is necessary. The final volume of each test concentration is 200 mL.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: chordat 86.81
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: A 7 day old strain is diluted with aqua bidest to a cell concentration of 10E5 cells/mL and than filled up to 1 L with 100 mL nutrient solution and deionised water. 50 mL of this solution is filled into 300 mL Erlenmeyer flasks and put into a breeding room at 23 ± 2°C for 3 days. The flasks are homogenised by daily swaying per hand. The different precultures were united again after 3 days. For inoculum the algae were diluted to 10E5 cell/mL again.

ACCLIMATION
- Culturing media and conditions: same as test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 ± 2 °C
pH:
no data
Nominal and measured concentrations:
nominal: 0, 1, 5, 10, 25, 50, 75, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type: closed with steel caps
- Size, fill volume: 300 mL flasks filled with 50 mL test solution
- Aeration: none
- Initial cells density: 10E5 cells/mL
- Control cells growth rate: 1.36
- No. of vessels per concentration (replicates): 4 replicates, 3 to measure cell density (via extinction during test period), 1 for measurement of extinction and pH at test start; furthermore 1 replicate with blank test concentrations (without inoculum)
- No. of vessels per control (replicates): 6
- No. of vessels per reference control (replicates): 4
- Homogenisation of algae suspension: by daily swaying per hand only once
- Growth medium: standard medium used

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: not stated
- Light intensity and quality: 85 µE/ m^2 s with neon tube (Osram L36W/ 25 universal white)

EFFECT PARAMETERS MEASURED: Cell density at test start and after 24, 48 and 72 h.
- Determination of cell concentrations: spectrophotometric (measurement of extinction at 578 nm)
Reference substance (positive control):
yes
Remarks:
Potassiumdichromate (K2Cr2O7)

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
25.72 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL: 19.408 - 33.836, taken from raw data
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
1.613 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL: 0.614 - 2.957, taken from raw data
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
4.39 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 95% CL: 1.813 - 7.395 taken from raw data
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.908 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 95% CL: 0.042 - 2.063, taken from raw data
Details on results:
- Exponential growth in the control: yes, growth rate 1.36/d (corresponding to a 59.9-fold growth)
Results with reference substance (positive control):
- Results with reference substance valid? Yes, inhibition of cell growth 62.4%.

Any other information on results incl. tables

Table 1: Inhibition of cell growth rate at different test concentrations within 72 h:

Test concentration [mg/L] Inhibition of growth rate [%]
1 -2.02
5 25.45
10 32.56
25 51.88
50 62.36
75 71.1
100 68.02

Table 2: Inhibition of cell growth rate by the reference substance within 72 h:

Test concentration [mg/L] Inhibition of growth rate [%]
0.5 21.46

Applicant's summary and conclusion