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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
27 Apr - 04 Jun 1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: All test concentrations incl. control
- Sampling method: Samples were taken after 0 and 48 h.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 120 mg/L was prepared. The test substance was weighed into water, treated for 60 sec at 8000 rpm with an ultra-turrax and afterwards stirred for 24 h on a magnetic stirrer. To remove undissolved particles the emulsion was filtered using a folded filter (pore size 7-12 µm).
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Cloned at the Federal Health Office (BGA), Berlin, Germany. Laboratory bred at Bayer AG, Leverkusen, Germany. Animals were kept in M4-medium according to Elendt and BGA (1992). Test animals were separated according to size before test start.
- Age at study initiation: < 24 h
- Feeding during test: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
15.7 °dH
Test temperature:
20.0 - 20.5 °C
pH:
7.8 - 8.0
Dissolved oxygen:
8.2 - 9.0 mg O2/L
Nominal and measured concentrations:
nominal: control, 5, 10, 20, 40 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: cylindrical test vessels, diameter: 4 cm, height: 6.5 cm, fill volume: 20 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED
- Mobility after 24 and 48 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
11.3 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Geometric mean of EC0 and EC100
Details on results:
- Mortality of control: 0%

BIOLOGICAL AND ANALYTICAL RESULTS:

Table 1: Immobilisation after 24 and 48 h.

Nominal test substance concentration [mg/L]

Immobilization of daphnids

Absolute

%

24 h

48 h

24 h

48 h

Control

0

0

0

0

5

0

0

0

0

10

2

8

10

40

20

20

20

100

100

40

20

20

100

100

 

Table 2: Results of the TOC analysis

Nominal test substance concentration [mg/L]

0 h

48 h

Control

< 2 mg/L

2 mg/L

5

4 mg/L

5 mg/L

10

7 mg/L

7 mg/L

20

12 mg/L

13 mg/L*

40

24 mg/L

24 mg/L*

* already measured after 24 h of exposure because of early 100% immobilization rates of the daphnids

VALIDITY CRITERIA:

Table 3: Validity criteria for OECD 202

Criterion from the guideline

Outcome

Validity criterion fulfilled

In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.

0% immobilization

yes

The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.

9 mg oxygen/L at the end of the test

yes

Validity criteria fulfilled:
yes
Remarks:
For further details please refer to “Any other information on results incl. tables”.
Conclusions:
In this study the substance was found to cause lethal effects to aq. invertebrates after 48 h exposure. The EC50 after 48 h was 11.3 mg test item/L (geometric mean of LC0 and LC100). The LC0 and LC100 after 48 h was 6.8 and 18.8 mg test item/L (measured test item concentrations), respectively.
Executive summary:

The short term toxicity of the substance to aquatic invertebrates and was performed according to the OECD guideline 202 and GLP. The water flea, Daphnia magna was exposed to nominal test item concentrations of 5, 10, 20, 40 mg/L and a control for 48 h. A static freshwater system was chosen for testing. The test item was monitored during the study via TOC analysis. The analytical recoveries were > 80% of the nominal values. The test item caused biological effects to Daphnia magna and resulted in an EC50 (48 h) of 11.3 mg/L (geom. mean meas.). The EC50 (48 h) of 11.3 mg/L (geom. mean meas.) was derived from the EC0 (48 h) = 6.8 mg/L and EC100 (48 h) = 18.8 mg/L.

Description of key information

EC50 (48 h) = 11.3 mg/L (geom. mean meas., D. magna, OECD 202)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
11.3 mg/L

Additional information

One study is available, testing the short term toxicity of the substance to aquatic invertebrates and was performed according to the OECD guideline 202 and GLP. The water flea, Daphnia magna was exposed to nominal test item concentrations of 5, 10, 20, 40 mg/L and a control for 48 h. A static freshwater system was chosen for testing. The test item was monitored during the study via TOC analysis. The analytical recoveries were > 80% of the nominal values. The test item caused biological effects to Daphnia magna and resulted in an EC50 (48 h) of 11.3 mg/L (geom. mean meas.). The EC50 (48 h) of 11.3 mg/L (geom. mean meas.) was derived from the EC0 (48 h) = 6.8 mg/L and EC100 (48 h) = 18.8 mg/L.