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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Vehicle:
yes
Remarks:
reconstituted water
Test organisms (species):
Daphnia magna
Details on test organisms:
Healthy Daphnia magna (showing no signs of stress such as presence of males and ephippia, delay in the production of the first brood, discolouration, etc. and having normal behavior) less than 24 h old (first instar nymph) cultured and bred in the Laboratory of Ecotoxicology, Jai Research Foundation, were used for the study. The master culture of Daphnia magna was originally procured from the MicroBio Test Inc, Kleimoer 15, 9030 Mariakerke (Gent), Belgium. The test system was validated for its sensitivity by conducting a validation study (Kamle, M.K., 2017: JRF Study N° 502-3-07-17313) with potassium dichromate as a positive control.
Gravid females were acclimatised to the test conditions for a minimum period of 48 h. During this period, Daphnia were fed with live algal cells (Pseudokirchneriella subcapitata). Freshly hatched daphnids belonging to the same stock (less than 24 h-old) were collected with the help of a micropipette and was used for the study. Feed was not provided during the exposure period.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
184.8 mg/L as CaCO3
Test temperature:
19.9 °C - 20.1 °C
pH:
7.44 - 7.70
Dissolved oxygen:
8.30 - 8.71 mg/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate as a positive control
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
mobility
Remarks on result:
other: LD50 (48 h) > 100 mg/L
Key result
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
mobility
Remarks on result:
other: LD 50 (24 h) > 100 mg/L
Validity criteria fulfilled:
yes
Conclusions:
EC50 (24 and 48 h), NOEC and LOEC: Greater than the test concentration of 100.0 mg test item/L.
EC50 (24 and 48 h), NOEC and LOEC: > 100.0 mg/L. (Dissolved fraction of Test substance)
Executive summary:

This study was performed to assess the toxic effects of the test substance on Daphnia magna under static conditions.

Based on the results of the preliminary range finding study, the main study was restricted to limit study and it was performed at the nominal test concentration of 100.0 mg/L. In the main study, a 48 h static toxicity test procedure was followed, comprising three groups. Each group consisted of four replicates with 5 daphnids per replicate.

As the study was conducted as a limit test of 100.0 mg test item/L and no immobility observed up to 48 h,

24 and 48 h, EC50 considered to be greater than test concentration of 100.0 mg test item/L as the test was performed for dissoved fraction of 100 mg test substance/ L.

Description of key information

This study was performed to assess the toxic effects of the test substance on Daphnia magna under static conditions.

Based on the results of the preliminary range finding study, the main study was restricted to limit study and it was performed at the nominal test concentration of 100.0 mg/L. In the main study, a 48 h static toxicity test procedure was followed, comprising three groups. Each group consisted of four replicates with 5 daphnids per replicate.

As the study was conducted as a limit test of 100.0 mg test item/L and no immobility observed up to 48 h,

24 and 48 h, EC50 considered to be greater than test concentration of 100.0 mg test item/L as the test was performed for dissoved fraction of 100 mg test substance/ L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

As the study was conducted as a limit test of 100.0 mg test item/L and no immobility observed up to 48 h,

24 and 48 h, EC50 considered to be greater than test concentration of 100.0 mg test item/L as the test was performed for dissoved fraction of 100 mg test substance/ L.