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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Version / remarks:
July 1992
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: domestic STP east of Suzhou city (no. 20150731)
- Preparation of inoculum for exposure: sedimented sludge was resuspended in test medium 3 times
- Pretreatment: none
- Concentration of sludge: 4.0 g suspended solid/L
Duration of test (contact time):
28 d
Initial conc.:
60 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: buffer solution with Ca, Mg, and Fe added
- Test temperature: 20+-1 °C
- pH: 7.49
- Aeration of dilution water:
- Suspended solids concentration: 30 mg/L

TEST SYSTEM
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: weekly
- Sampling method: BOD analyzer

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (O2 consumption)
Value:
22.4
Sampling time:
18 d
Remarks on result:
other: 10-d time window began between D8 (9.4%) and D9 (11.2% degr.)
Key result
Parameter:
% degradation (O2 consumption)
Value:
21.6
Sampling time:
19 d
Results with reference substance:
83.5% degradation on D14
Validity criteria fulfilled:
yes
Remarks:
1) Difference between replicates was 2.9% (<20%) 2) Ref.item was degraded by 83.5% in 14 days ( min.60%) 3) Avg. O2 uptake of inoculum blank was 21.2 mg/L in 28 days (max. 60 mg/L) 4) Toxicity control showed 52.1% degradation on D14 (min. 25%)
Interpretation of results:
inherently biodegradable
Conclusions:
Di-PETMP is not readily biodegradable, but can be assumed to be inherently biodegradable with > 20% biodegradation.

Description of key information

Di-PETMP is not readily biodegradable in a manometric respiration test. However, it can be assumed inherently biodegradable with > 20% biodegradation.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable
Type of water:
freshwater

Additional information