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Bioaccumulation: aquatic / sediment

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Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
Test method according to OECD guideline 305 C. No data on GLP.
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
Deviations:
not specified
GLP compliance:
not specified
Test organisms (species):
Cyprinus carpio
Route of exposure:
aqueous
Test type:
not specified
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk
Test temperature:
25 °C
Nominal and measured concentrations:
15 µg/L and 1.5 µg/L.
Reference substance (positive control):
not specified
Lipid content:
4.2 %
Key result
Conc. / dose:
15 µg/L
Temp.:
ca. 25 °C
Type:
BCF
Value:
>= 432 - <= 922 L/kg
Key result
Conc. / dose:
1.5 µg/L
Temp.:
ca. 25 °C
Type:
BCF
Value:
>= 606 - <= 1 290 L/kg

BCF values of 432 -922 L/kg and 606 -1290 L/kg were measured in fish for Camphene at concentrations of 15 and 1.5 µg/L, respectively.

Validity criteria fulfilled:
not specified
Conclusions:
BCF values of 432 -922 L/kg and 606 -1290 L/kg were measured in fish for Camphene at concentrations of 15 and 1.5 µg/L, respectively.
Executive summary:

BCF values of 432 -922 L/kg and 606 -1290 L/kg were measured in fish for Camphene at concentrations of 15 and 1.5 µg/L, respectively, using carp (Cyrpinus carpio) which were exposed over a 8 -week period. Test method was according to OECD guideline 305 C.

Endpoint:
bioaccumulation in aquatic species, other
Type of information:
(Q)SAR
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Remarks:
Internationally accepted method, EPI Suite, EPA (USA)
Justification for type of information:
See attached the QMRF and QPRF for the QSAR model.
Guideline:
other: REACH Guidance on QSARs R.6
Principles of method if other than guideline:
Meylan, W.M., Howard, P.H, Aronson, D., Printup, H. and S. Gouchie. 1997. "Improved Method for Estimating Bioconcentration Factor (BCF) from Octanol-Water Partition Coefficient", SRC TR-97-006 (2nd Update), July 22, 1997; prepared for: Robert S. Boethling, EPA-OPPT, Washington, DC; Contract No. 68-D5-0012; prepared by: ; Syracuse Research Corp., Environmental Science Center, 6225 Running Ridge Road, North Syracuse, NY 13212.
Meylan, W.M., Howard, P.H., Boethling, R.S. et al. 1999. Improved Method for Estimating Bioconcentration / Bioaccumulation Factor from Octanol/Water Partition Coefficient. Environ. Toxicol. Chem. 18(4): 664-672 (1999).
Specific details on test material used for the study:
SMILES: C(C(CC1C2)C2)(C1(C)C)=C
Key result
Type:
BCF
Value:
282.7 L/kg
Remarks on result:
other: (Log BCF from regression-based method = 2.451; log Kow used: 4.22)

The BCF of D-camphene was 282.7 L/kg wet-wt (log BCF from regression-based method = 2.451).

Validity criteria fulfilled:
not applicable
Conclusions:
The BCF of D-camphene was 282.7 L/kg wet-wt (log BCF from regression-based method = 2.451).
Executive summary:

Using the BCFBAF v3.01 model of EPI Suite v4.11, the BCF of D-camphene was 282.7 L/kg wet-wt (log BCF from regression-based method = 2.451).

Endpoint:
bioaccumulation in aquatic species, other
Type of information:
(Q)SAR
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Remarks:
Internationally accepted method, EPI Suite, EPA (USA)
Justification for type of information:
See attached the QMRF and QPRF for the QSAR model.
Guideline:
other: REACH Guidance on QSARs R.6
Principles of method if other than guideline:
Meylan, W.M., Howard, P.H, Aronson, D., Printup, H. and S. Gouchie. 1997. "Improved Method for Estimating Bioconcentration Factor (BCF) from Octanol-Water Partition Coefficient", SRC TR-97-006 (2nd Update), July 22, 1997; prepared for: Robert S. Boethling, EPA-OPPT, Washington, DC; Contract No. 68-D5-0012; prepared by: ; Syracuse Research Corp., Environmental Science Center, 6225 Running Ridge Road, North Syracuse, NY 13212.
Meylan, W.M., Howard, P.H., Boethling, R.S. et al. 1999. Improved Method for Estimating Bioconcentration / Bioaccumulation Factor from Octanol/Water Partition Coefficient. Environ. Toxicol. Chem. 18(4): 664-672 (1999).
Specific details on test material used for the study:
SMILES: C(CC1C2)C(C2=C)C1(C)C
Key result
Type:
BCF
Value:
343 L/kg
Remarks on result:
other: (Log BCF from regression-based method = 2.535; log Kow used: 4.35)

The BCF of fenchene was 343 L/kg wet-wt (log BCF from regression-based method = 2.535).

Validity criteria fulfilled:
not applicable
Conclusions:
The BCF of fenchene was 343 L/kg wet-wt (log BCF from regression-based method = 2.535).
Executive summary:

Using the BCFBAF v3.01 model of EPI Suite v4.11, the BCF of D-camphene was 343 L/kg wet-wt (log BCF from regression-based method = 2.535).

Endpoint:
bioaccumulation in aquatic species, other
Type of information:
(Q)SAR
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Remarks:
Internationally accepted method, EPI Suite, EPA (USA)
Justification for type of information:
See attached the QMRF and QPRF for the QSAR model.
Guideline:
other: REACH Guidance on QSARs R.6
Principles of method if other than guideline:
Meylan, W.M., Howard, P.H, Aronson, D., Printup, H. and S. Gouchie. 1997. "Improved Method for Estimating Bioconcentration Factor (BCF) from Octanol-Water Partition Coefficient", SRC TR-97-006 (2nd Update), July 22, 1997; prepared for: Robert S. Boethling, EPA-OPPT, Washington, DC; Contract No. 68-D5-0012; prepared by: ; Syracuse Research Corp., Environmental Science Center, 6225 Running Ridge Road, North Syracuse, NY 13212.
Meylan, W.M., Howard, P.H., Boethling, R.S. et al. 1999. Improved Method for Estimating Bioconcentration / Bioaccumulation Factor from Octanol/Water Partition Coefficient. Environ. Toxicol. Chem. 18(4): 664-672 (1999).
Specific details on test material used for the study:
SMILES: C(C(CC1C2)C1(C)C)(=C2)C
Key result
Type:
BCF
Value:
394.9 L/kg
Remarks on result:
other: (Log BCF from regression-based method = 2.597; log Kow used: 4.44)

The BCF of L-alpha pinene was 394.9 L/kg wet-wt (log BCF from regression-based method = 2.597).

Validity criteria fulfilled:
not applicable
Conclusions:
The BCF of L-alpha pinene was 394.9 L/kg wet-wt (log BCF from regression-based method = 2.597).
Executive summary:

Using the BCFBAF v3.01 model of EPI Suite v4.11, the BCF of L-alpha pinene was 394.9 L/kg wet-wt (log BCF from regression-based method = 2.597).

Endpoint:
bioaccumulation in aquatic species, other
Type of information:
(Q)SAR
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Remarks:
Internationally accepted method, EPI Suite, EPA (USA)
Justification for type of information:
See attached the QMRF and QPRF for the QSAR model.
Guideline:
other: REACH Guidance on QSARs R.6
Principles of method if other than guideline:
Meylan, W.M., Howard, P.H, Aronson, D., Printup, H. and S. Gouchie. 1997. "Improved Method for Estimating Bioconcentration Factor (BCF) from Octanol-Water Partition Coefficient", SRC TR-97-006 (2nd Update), July 22, 1997; prepared for: Robert S. Boethling, EPA-OPPT, Washington, DC; Contract No. 68-D5-0012; prepared by: ; Syracuse Research Corp., Environmental Science Center, 6225 Running Ridge Road, North Syracuse, NY 13212.
Meylan, W.M., Howard, P.H., Boethling, R.S. et al. 1999. Improved Method for Estimating Bioconcentration / Bioaccumulation Factor from Octanol/Water Partition Coefficient. Environ. Toxicol. Chem. 18(4): 664-672 (1999).
Specific details on test material used for the study:
SMILES: C(C(CC1C2)C2)(C1(C)C)=C
Key result
Type:
BCF
Value:
282.7 L/kg
Remarks on result:
other: (Log BCF from regression-based method = 2.451; log Kow used: 4.22)

The BCF of L-camphene was 282.7 L/kg wet-wt (log BCF from regression-based method = 2.451).

Validity criteria fulfilled:
not applicable
Conclusions:
The BCF of L-camphene was 282.7 L/kg wet-wt (log BCF from regression-based method = 2.451).
Executive summary:

Using the BCFBAF v3.01 model of EPI Suite v4.11, the BCF of L-camphene was 282.7 L/kg wet-wt (log BCF from regression-based method = 2.451).

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
The analogue substance camphene which shares the same functional groups with the main components of the multi-constituent substance reaction mass of fenchene and laevo camphene and dextro camphene and laevo alpha pinene also has comparable values for the relevant molecular properties.
See attached the reporting format.
Reason / purpose for cross-reference:
read-across source
Lipid content:
4.2 %
Key result
Conc. / dose:
15 µg/L
Temp.:
ca. 25 °C
Type:
BCF
Value:
>= 432 - <= 922 L/kg
Remarks on result:
other: read-across from an analogue for which BCF =432-922 L/kg
Key result
Conc. / dose:
1.5 µg/L
Temp.:
ca. 25 °C
Type:
BCF
Value:
>= 606 - <= 1 290 L/kg
Remarks on result:
other: read-across from an analogue for which BCF =606-1260 L/kg
Validity criteria fulfilled:
not applicable
Conclusions:
Based on the read-across approach from experimental data on the analogue camphene, the BCF value of the reaction mass was determined as a maximum of 1290 L/kg.
Executive summary:

BCF values of 432 -922 L/kg and 606 -1290 L/kg were measured in fish for Camphene at concentrations of 15 and 1.5 µg/L, respectively, using carp (Cyrpinus carpio) which were exposed over a 8 -week period. Test method was according to OECD guideline 305 C. Based on these results, the read-across approach was applied and the BCF of the reaction mass was determined as a maximum of 1290 L/kg.

Description of key information

Key study. Read-across approach. Test method OECD guideline 305 C. Based on read-across from experimental data on analogue camphene, the BCF value of the reaction mass was determined as a maximum of 1290 L/kg.

Supporting studies: Data from EPI-Suite, EPA (USA) / BCFBAF v3.01 calculation method. The BCF of the main components of the reaction mass were calculated to be in the range of 283 -395 L/kg.

Key value for chemical safety assessment

BCF (aquatic species):
1 290 L/kg ww

Additional information

Key study. Read-across approach. BCF values of 432 -922 L/kg and 606 -1290 L/kg were measured in fish for Camphene at concentrations of 15 and 1.5µg/L, respectively, using carp (Cyrpinus carpio) which were exposed over a 8 -week period. Test method was according to OECD guideline 305 C. Based on these results, the read-across approach was applied and the BCF of the reaction mass was determined as a maximum of 1290 L/kg.

Supporting studies: Data from EPI-Suite, EPA (USA) / BCFBAF v3.01 calculation method. The BCF of the main components of the reaction mass were calculated to be in the range of 283 -395 L/kg.

According to Annex XIII to REACH, the substance is considered not to fulfil the criterion for bioaccumulation, since the estimated BCF (BCF = 1290 L/kg wet-wt) is < 2000.