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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17 March 1996 to 21 March 1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
not specified
Analytical monitoring:
no
Details on sampling:
Not applicable
Vehicle:
no
Details on test solutions:
The substance was dispersed in 1 L of algal medium. The mixtures were stirred with a magnetstirrer for 48 hours. These solutions were used as stock solutions (100 mg/L) for acute toxicity testing.
Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Selenastrum capricornutum
- Strain: not stated
- Source (laboratory, culture collection): laboratory culture, obtained from the Institute of Applied Environmental Research, Stockholm, Sweden
- Age of inoculum (at test initiation): not stated
- Method of cultivation: not stated

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Remarks on exposure duration:
Not applicable
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
22 ± 1°C
pH:
Start of the study the pH was 8.0
At the end of the study the pH ranged between 8.0 and 9.2.
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Conductivity:
Not applicable
Nominal and measured concentrations:
Nominal exposure concentrations were 6.25, 12.5, 25, 50 & 100 mg/L.
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL glass E-bottles
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 mL
- Initial cells density: not stated
- Control end cells density: not stated
- No. of organisms per vessel: not stated
- No. of vessels per concentration (replicates):5
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: algal growth medium

OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH:
- Photoperiod: continuous
- Light intensity and quality: 100 µEm-2 s-1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll a measurement

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: not reported
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
The 72 hour EC50 was graphically calculated to be >100 mg/L and the corresponding EC10 was graphically calculated to be 37 mg/L or 32 mg/L using probit analysis.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Samples were analysed for chlorophyll a fluorescence. The growth rates were between 0 and 72 hours were calculated with log2 for the average of the fluorescence for each test concentration. Results were determined by graphical interpolation and probit analysis.

Algal test results

Nominal conc (mg/L)

Fluorescence (chlorophyll a)

(µ) growth rate 0-72h

% inhibition

0 h

24 h

48 h

72 h

 

 

Control

1.1

3.0

14.9

72.6

2.01

 

1.1

3.1

16.6

75.6

2.03

1.1

3.0

16.2

74.7

2.03

1.0

3.3

16.0

75.6

2.08

1.1

3.1

16.0

75.6

2.03

Mean

1.08

3.1

15.94

74.82

2.04

 

 

6.25

1.1

2.8

11.6

54.9

1.88

7.74

1.1

2.9

12.6

62.7

1.94

4.60

1.1

3.2

13.4

66.9

1.98

3.07

Mean

1.10

2.97

12.53

61.5

1.94

5.06

 

12.5

1.1

3.1

14.8

69.6

1.99

2.14

1.2

3.2

14.8

67.2

1.94

5.02

1.2

3.1

15.3

72.0

1.97

3.39

Mean

1.17

3.13

14.97

69.6

1.97

3.53

 

25

1.2

2.9

13.4

63.0

1.90

6.54

1.2

3.1

13.7

62.4

1.90

6.77

1.2

3.0

14.0

64.2

1.91

6.10

Mean

1.2

3.0

13.7

63.2

1.91

6.47

 

50

1.2

3.8

10.2

44.4

1.74

14.8

1.2

2.8

10.7

49.8

1.79

12.09

1.2

2.8

11.0

50.4

1.80

11.81

Mean

1.2

3.13

10.63

48.2

1.78

12.86

 

100

1.2

2.9

6.8

19.2

1.33

34.58

1.2

2.5

6.7

19.6

1.34

34.09

1.2

2.5

6.7

19.2

1.33

34.58

Mean

1.2

2.63

6.73

19.33

1.34

34.42

 

Validity criteria fulfilled:
yes
Conclusions:
The 72 hour EC50 was graphically calculated to be >100 mg/L and the corresponding EC10 was graphically calculated to be 37 mg/L or 32 mg/L using probit analysis.
Executive summary:

The inhibition of growth of 1,3-propanediol, 2,2- bis(hydroxymethyl) -, allyl ether to the green algae Selenastrum capricornutum was determined. The study was carried out according to OECD test guideline 201 – Freshwater Alga and Cyanobacteria, Growth Inhibition Test. In this study algae were exposed to the test material over a test period of 72 hours under static conditions. The test material was dispersed in 1 L algal medium; the mixtures were stirred for 48 hours. These solutions were used as stock solutions (100 mg/L) for acute toxicity testing.

Nominal exposure concentrations were 0 (control), 6.25, 12.5, 25, 50 and 100 mg/L.  The results of the study were based on the nominal concentrations.

The 72 hour EC50 was graphically calculated to be >100 mg/L and the corresponding EC10 was graphically calculated to be 37 mg/L or 32 mg/L using probit analysis.

Description of key information

The 72 hour EC50 was graphically calculated to be >100 mg/L and the corresponding EC10 was calculated to be 32 mg/L using probit analysis.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L
EC10 or NOEC for freshwater algae:
32 mg/L

Additional information

The inhibition of growth of 1,3-propanediol, 2,2- bis(hydroxymethyl) -, allyl ether to the green algae Selenastrum capricornutum was determined. The study was carried out according to OECD test guideline 201 – Freshwater Alga and Cyanobacteria, Growth Inhibition Test. In this study algae were exposed to the test material over a test period of 72 hours under static conditions. The test material was dispersed in 1 L algal medium; the mixtures were stirred for 48 hours. These solutions were used as stock solutions (100 mg/L) for acute toxicity testing.

Nominal exposure concentrations were 0 (control), 6.25, 12.5, 25, 50 and 100 mg/L.  The results of the study were based on the nominal concentrations.

The 72 hour EC50 was graphically calculated to be >100 mg/L and the corresponding EC10 was graphically calculated to be 37 mg/L or 32 mg/L using probit analysis.