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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Zanthoxylum, ext.
EC Number:
310-094-8
EC Name:
Zanthoxylum, ext.
Cas Number:
102242-62-6
Molecular formula:
NA
IUPAC Name:
Zanthoxylum ext
Test material form:
liquid: viscous

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
other: ISO medium, according to OECD 202
Total exposure duration:
48 h

Test conditions

Hardness:
The reconstituted water (ISO medium) has an approximate theoretical total hardness of 249 mg/L (as CaCO3).
Test temperature:
The test temperature was in the range of 19.1 – 19.4°C measured in the test vessels during the test.
pH:
The pH was in the range of 7.78 – 8.05 during the test.
Dissolved oxygen:
The dissolved oxygen concentration was in the range of 7.10 – 7.35 mg/L during the test.
Nominal and measured concentrations:
The following nominal concentrations were tested: 0.625, 1.25, 2.5, 5.0 and 10.0 mg/L.

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
3.07 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
other: EC20
Effect conc.:
3.37 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In this 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the test item Zanthoxylum extract had significant toxic effects on the mobility of Daphnia. The 48-h EC50 value was determined to be 4.01 mg/L.
The 48-h NOEC was determined to be 2.5 mg/L and the LOEC was determined to be 5.0 mg/L.
All validity criteria were met. The results are based on measured test item concentrations.