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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015-06-16 to 2015-07-28
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 425 (Acute Oral Toxicity: Up-and-Down Procedure)
GLP compliance:
yes (incl. QA statement)
Test type:
up-and-down procedure
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
4-aminobenzonitrile
EC Number:
212-850-1
EC Name:
4-aminobenzonitrile
Cas Number:
873-74-5
Molecular formula:
C7H6N2
IUPAC Name:
4-aminobenzonitrile
Test material form:
solid: flakes

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
Temperature 22+/-3°C
Humidity 55+/- 10%
Light 12h/Dark 12h
Animals 9 to 11 weeks old

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Doses:
Low dose 175 mg/kg, high dose 550 mg/kg
No. of animals per sex per dose:
3 animals per dose
Control animals:
no

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
ca. 310 mg/kg bw
Based on:
test mat.
Mortality:
3 mortalities at 550 mg/kg
0 mortalities at 175 mg/kg
Clinical signs:
other: The most relevant clinical findings in the animals treated with 175 mg/kg were: reduced spontaneous activity, prone position, bradykinesia, cyanosis, eyes half closed, abmoral breathing, ataxia, piloerection, wasp waist, kyphosis and transient weight loss
Gross pathology:
no specific findings

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The LD50 of 4-Aminobenzonitrile was found to be 310.2 mg/kg which leads to the classification acute toxicity category 4 according to CLP Regulation.
Executive summary:

All animals used in the study were allowed to acclimatise to the laboratory conditions for at least 5 days after entrance at BSL. The animals were observed on delivery, on inclusion in the study and before administration for mortality/morbidity and other clincal signs. All animals were examined for clinical signs several times on the day of dosing and once daily until the end of the observation period. Their body weights were recorded on day 1 (prior to the administration) and on days 8 and 15. All animals were necropsied and examined macroscopically.

On the basis of the results given and in conformity with the criteria given in Annex I of Regulation (EC) 1272/2008 the substance should be classified into category 4.