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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study, GLP, All validity criteria fulfilled, complete identification of test substance, including chemical analyses
Justification for type of information:
Read-across data is presented from quaternary ammonium compounds, C12-18-alkylbis(hydroxyethyl)methyl, chlorides, CAS Number 71808-53-2, EC Number 276-038-9. This substance is considered to be close enough in structural integrity to the target substance, bis(2 -hydroxyethyl)oleylmethylammonium chloride, CAS Number 18448 -65 -2, EC Number 242 -332 -0, so as to justify valid read-across.

The 21d-EC50 determined in a long term OECD 211 test (reliability 1) is used as a worst case key value for the short term toxicity to aquatic invertebrates.
Qualifier:
according to guideline
Guideline:
other: OECD Guideline 211 (Daphnia magna Reproduction Test)
Version / remarks:
The 21d-EC50 determined in a long term OECD 211 test (reliability 1) is used as a worst case key value for the short term toxicity to aquatic invertebrates.
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
- Name of test material (as cited in study report): Cocobis(2-hydroxyethyl) methylammonium chloride (CAS No. 70750-47-9)
- Physical state: Paste-like, yellow-brownish
- Active ingredient content: 99.4%
97.9 % Cocobis(2-hydroxyethyl) methylammonium chloride
1.5 % Cocobis(2-hydroxyethyl) amine
- Purity test date: 2009-06-02
- Lot/batch No.: S001700
- Expiration date of the lot/batch: 2019-04-28
- Storage condition of test material: Room temperature, protected from moisture and light, under nitrogen gas.
Test organisms (species):
Daphnia magna
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Key result
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
465 µg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: (268 - 805 ug/L)
Key result
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
468 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (270 - 810 ug/L)
Validity criteria fulfilled:
yes
Remarks:
100% survival in control
Conclusions:
The short-term toxicity 48h-EC50 for Daphnia magna is conservatively derived from the results of the 21d-reproduction study with the same species. This is a valid study, under GLP and Reliability 1. The EC50 was 468 ug/L, with 95%-confidence limits 270 - 810 ug/L.
Executive summary:

The short-term toxicity to aquatic invertebrates of the test substance, Quaternary ammonium compounds, coco alkylbis(hydroxyethyl)methyl chlorides, CAS Number 70750 -47 -9, EC Number 274-846-6, [Quaternary ammonium compounds, C12-18-alkylbis(hydroxyethyl)methyl, chlorides, CAS Number 71808 -53 -2, EC Number 276 -038 -9], was investigated in a 21 d-reproduction study conducted according to OECD guideline 211 (Daphnia Magna Reproduction Test) - the short-term toxicity (48h - EC50) being conseravtively derived.

The study was assigned a reliability score of 1 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).

The survival after 21 days in the long-term toxicity study with Daphnia magna (according to OECD TG 211), 21d-EC50 was taken as a conservative estimate for the short-term toxicity to Daphnia magna (48h-EC50). Details of the study are recorded under the section for long-term toxicity. The EC50 was 468 ug/l, with 95%-confidence limits 270 - 810 ug/L.

Description of key information

The short-term toxicity to aquatic invertebrates of the test substance, Quaternary ammonium compounds, coco alkylbis(hydroxyethyl)methyl chlorides, CAS Number 70750 -47 -9, EC Number 274-846-6, [Quaternary ammonium compounds, C12-18-alkylbis(hydroxyethyl)methyl, chlorides, CAS Number 71808 -53 -2, EC Number 276 -038 -9], was investigated in a 21 d-reproduction study conducted according to OECD guideline 211 (Daphnia Magna Reproduction Test) - the short-term toxicity (48h - EC50) being conseravtively derived.

This substance is considered to be close enough in structural integrity to the target substance, bis(2 -hydroxyethyl)oleylmethylammonium chloride, CAS Number 18448 -65 -2, EC Number 242 -332 -0, so as to justify valid read-across.

The study was assigned a reliability score of 1 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).

The survival after 21 days in the long-term toxicity study with Daphnia magna (according to OECD TG 211), 21d-EC50 was taken as a conservative estimate for the short-term toxicity to Daphnia magna (48h-EC50). Details of the study are recorded under the section for long-term toxicity. The EC50 was 468 ug/l, with 95%-confidence limits 270 - 810 ug/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.468 mg/L

Additional information

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