Bis(hydroxyethyl)methyloleylammonium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, GLP, All validity criteria fulfilled, complete identification of test substance, including chemical analyses

Justification for type of information:

Read-across data is presented from quaternary ammonium compounds, C12-18-alkylbis(hydroxyethyl)methyl, chlorides, CAS Number 71808-53-2, EC Number 276-038-9. This substance is considered to be close enough in structural integrity to the target substance, bis(2 -hydroxyethyl)oleylmethylammonium chloride, CAS Number 18448 -65 -2, EC Number 242 -332 -0, so as to justify valid read-across.

The 21d-EC50 determined in a long term OECD 211 test (reliability 1) is used as a worst case key value for the short term toxicity to aquatic invertebrates.

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

The 21d-EC50 determined in a long term OECD 211 test (reliability 1) is used as a worst case key value for the short term toxicity to aquatic invertebrates.

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): Cocobis(2-hydroxyethyl) methylammonium chloride (CAS No. 70750-47-9)
- Physical state: Paste-like, yellow-brownish
- Active ingredient content: 99.4%
97.9 % Cocobis(2-hydroxyethyl) methylammonium chloride
1.5 % Cocobis(2-hydroxyethyl) amine
- Purity test date: 2009-06-02
- Lot/batch No.: S001700
- Expiration date of the lot/batch: 2019-04-28
- Storage condition of test material: Room temperature, protected from moisture and light, under nitrogen gas.

Test organisms (species):

Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

465 µg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: (268 - 805 ug/L)

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

468 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: (270 - 810 ug/L)

Validity criteria fulfilled:

yes

Remarks:

100% survival in control

Conclusions:

The short-term toxicity 48h-EC50 for Daphnia magna is conservatively derived from the results of the 21d-reproduction study with the same species. This is a valid study, under GLP and Reliability 1. The EC50 was 468 ug/L, with 95%-confidence limits 270 - 810 ug/L.

Executive summary:

The short-term toxicity to aquatic invertebrates of the test substance, Quaternary ammonium compounds, coco alkylbis(hydroxyethyl)methyl chlorides, CAS Number 70750 -47 -9, EC Number 274-846-6, [Quaternary ammonium compounds, C12-18-alkylbis(hydroxyethyl)methyl, chlorides, CAS Number 71808 -53 -2, EC Number 276 -038 -9], was investigated in a 21 d-reproduction study conducted according to OECD guideline 211 (Daphnia Magna Reproduction Test) - the short-term toxicity (48h - EC50) being conseravtively derived.

The study was assigned a reliability score of 1 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).

The survival after 21 days in the long-term toxicity study with Daphnia magna (according to OECD TG 211), 21d-EC50 was taken as a conservative estimate for the short-term toxicity to Daphnia magna (48h-EC50). Details of the study are recorded under the section for long-term toxicity. The EC50 was 468 ug/l, with 95%-confidence limits 270 - 810 ug/L.

Description of key information

The short-term toxicity to aquatic invertebrates of the test substance, Quaternary ammonium compounds, coco alkylbis(hydroxyethyl)methyl chlorides, CAS Number 70750 -47 -9, EC Number 274-846-6, [Quaternary ammonium compounds, C12-18-alkylbis(hydroxyethyl)methyl, chlorides, CAS Number 71808 -53 -2, EC Number 276 -038 -9], was investigated in a 21 d-reproduction study conducted according to OECD guideline 211 (Daphnia Magna Reproduction Test) - the short-term toxicity (48h - EC50) being conseravtively derived.

This substance is considered to be close enough in structural integrity to the target substance, bis(2 -hydroxyethyl)oleylmethylammonium chloride, CAS Number 18448 -65 -2, EC Number 242 -332 -0, so as to justify valid read-across.

The study was assigned a reliability score of 1 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).

The survival after 21 days in the long-term toxicity study with Daphnia magna (according to OECD TG 211), 21d-EC50 was taken as a conservative estimate for the short-term toxicity to Daphnia magna (48h-EC50). Details of the study are recorded under the section for long-term toxicity. The EC50 was 468 ug/l, with 95%-confidence limits 270 - 810 ug/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.468 mg/L