Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-055-6 | CAS number: 132-27-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- Only three test substance concentrations were tested. According to OECD Guideline 211, normally there should be at least five test concentrations arranged in a geometric series with a separation factor preferably not exceeding 3.2.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.01, 0.03, 0.1 mg a.i./L at day 0, 7, and 16 in new media; at day 2, 9, 19 in old media
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared to give the desired series of test concentrations. 10 mg of the test susbstance were added to 1 L of dilution water and treated for 1 h with and ultrasonic bath and afterwards stirred for 24 h. Finally undissolved particles of the test substance were removed by filtration. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Bundesgesundheitsamt, Berlin, Germany; maintained since more than 15 years in the test facility
- Age of parental stock: ≤ 24 h
- Food type: Scenedesmus subspicatus
- Amount and frequency: day 1-7: 0.1 mg C/Daphnia/day; day 8-21: 0.2 mg C/Daphnia/day - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 257 - 280.2 mg/L as CaCO3
- Test temperature:
- 25 ± 1 °C
- pH:
- 8.0 ± 0.2
- Dissolved oxygen:
- Control: 8.5 - 9.2 mg/L
Highest test concentration: 8.4 - 9.2 mg/L - Nominal and measured concentrations:
- Nominal test cocnentrations: control, 0.01, 0.03, 0.1 mg a.i./L
Mean measured concentrations: -, 0.009, 0.022, 0.07 mg a.i./L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, nominal volume, fill volume: glass beakers, 150 ml, 100 ml
- Aeration: none
- Renewal rate of test solution (frequency): three times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstitued water (M4-medium)
- Intervals of water quality measurement: Dissolved oxygen concentration, water temperature and pH-value were measured once a week in old and fresh media, in one replicate of the control and highest test substance concentration.
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h light-darkness cycle
- Light intensity: not exceeding 800 lux
EFFECT PARAMETERS MEASURED: Number of living offspring per parent animal and behaviour/mortality rate of parent Daphnia three times a week - Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.022 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.009 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.006 other: mg a.i./L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals: none
- Time to first brood release: 5 days
- Mean reproduction rates: 88.4%, 84.3% and 81.5% for the 0.01, 0.03 and 0.1 mg/L concentrations, respectively - Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to analogue justification provided in IUCLID section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.022 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.009 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.006 other: mg a.i./L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
Referenceopen allclose all
For the assessment the NOEC was recalculated based on the geometric mean of the measured concentrations. The geometric mean measured concentrations were: 0.006, 0.011 and 0.0024 mg a.i./L.
Description of key information
NOEC (21 d): 0.006 mg a.i./L (measured, geom. mean); read across
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.006 mg/L
Additional information
Data on the chronic toxicity of sodium 2-biphenylate (CAS 132-27-4) to aquatic invertebrates are not available. The assessment was therefore based on a study conducted with the analogue substance 2-phenylphenol (CAS 90-43-7) as part of a read across approach, which is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Further justification is given within the overall summary chapter 6.1 and within the analogue justification in IUCLID section 13.
The chronic toxicity of substance 2-phenylphenol (CAS 90-43-7) was investigated in a guideline study performed according to OEC 211 and in GLP compliance (Bruns, 2001).Daphnia magna was exposed to the substance for 21 days under semi-static exposure conditions. The tested nominal concentrations were 0.01, 0.03, and 0.1 mg a.i./L. Analytical dose verification was performed with fresh and for aged medium respectively. The fresh medium was sampled on day 0, 7, and 16, whereas the old medium was sampled at day 2, 9, and 19. Based on the arithmetic mean the analytically verified concentrations were 0.009, 0.022, 0.07 mg a.i./L. For the assessment the NOEC was recalculated based on the geometric mean of the measured concentrations. The geometric mean measured concentrations were: 0.006, 0.011 and 0.0024 mg a.i./L. Based geometric mean measured concentrations the NOECreproduction(21 d)was 0.006 mg a.i./L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
