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EC number: 205-055-6 | CAS number: 132-27-4
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with some missing details
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct weighing, the test substance was added to abput 130 ml deionzed water and stirred before testing for 2 hours. - Test organisms (species):
- activated sludge of a predominantly industrial sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: The sludge is settled and supernatant is decanted. After centrifuging (20 min at 4000 rpm and 20°C) the supernatant is decanted again. 1 g of wet sludge is dried in order to calculate the amount of wet sludge to achieve a concentration of activated sludge of 6 g/L (dry wet) suspended solids. The calculated amount of sludge is first dissolved in synthetic medium and then filled up to a defined end volume with deionised water.
- Initial biomass concentration: 6 g/L dw suspended solids - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20°C ± 2 °C
- pH:
- 7.1 - 8.0
- Nominal and measured concentrations:
- Nominal test concentrations: control, 10, 18, 56, 100 mg a.i./L
- Details on test conditions:
- TEST SYSTEM
- Biomass loading rate: 6 g/L dw suspended solids
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : respiration inhibition after 3 hours
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations:
- First range-finding test:100, 1000, 10000 mg/L
- Second range-finding test: 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Since the first range-finding test showed an inhibition greater than 50% at 100 mg/L, a second range-finding test with lower test substance concentrations was conducted. The second test revealed inhibition rates of 0 % and 1.5 % for test substances concentrations of 1 and 10 mg/L, respectively - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: EC05
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 31 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 62.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC50 = 9.2 mg/L
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to analogue justification provided in IUCLID section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- other: EC05
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 31 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 62.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Referenceopen allclose all
Table 1: Respiratory rates, oxygen-consumption and respiration inhibition of the treated activated sludge
Nominal test concentration [mg a.i./L] |
Respiratory rate [mg/L x h] |
Phys.-chem. O2consumption |
Respiratory rateminusphys.-chem. O2consumption |
Inhibition [%] |
10 18 32 56 100 |
20 18 19 9.4 3.0 |
0.0* 0.0* 0.0* 0.0* 0.0* |
20 18 19 9.4 3.0 |
0 0 0 47.6 83.3 |
Control 1 Control 2 Control, mean |
18 18 18 |
|
|
|
Description of key information
EC10 (3 h): 31 mg/L (read across)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 62.2 mg/L
- EC10 or NOEC for microorganisms:
- 31 mg/L
Additional information
Reliable data on the toxicity of sodium 2-biphenylate (CAS 132-27-4) to microorganisms are not available. The assessment was therefore based on a study conducted with the analogue substance 2-phenylphenol (CAS 90-43-7) as part of a read across approach, which is in accordance with Regulation (EC) No 1907/2006, Annex XI, 1.5. Further justification is given within the overall summary chapter 6.1 and within the analogue justification in IUCLID section 13.
The effect of 2-phenylphenol (CAS 90-43-7) on aquatic micro-organism was investigated according to ISO guideline 8192 comparable with OECD 209 (Mueller, 1990). The study was re-evaluated according to recent regulatory requirements by Weyers (2006). At nominal test concentrations of 10 - 100 mg/L, inhibition of respiration in activated sludge was observed between 0% and 83.3% and a dose - response relationship was demonstrated. An inhibition of microbial activity by 50% was determined at EC50(3 h) = 62.2 mg/L. The EC10(3 h) of 31 mg/L was used for further assessment.
Additional information is available from a study investigating the toxicity of sodium 2-biphenylate toPseudomonas fluorescensandEscherichia coli(Hamburger, 1974). The study followed the modified German Uniform Procedure L8 and determined no adverse effects onE. coliup to 10 g/L and toxic effects onP. fluorescensat concentrations ≥ 1 g/L.
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