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EC number: 205-055-6 | CAS number: 132-27-4
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Storage condition of test material: room temperature
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): The test item was dissolved in an amount of acteone sufficient to prepare a stock solution. An appropiate amount of the solution were used to soak a portion of quartz sand. After evaporation of the solvent, the test item coated fraction of the quartz sand was incorporated into the remainder of the artifical soil by thoroughly mixing it for approx. 10 min.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Evaporation of vehicle before use: yes - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: Co. Landenberger, 72355 Schömberg, Germany, kept at ECT Oekotoxikologie GmbH
- Age at test initiation (mean and range, SD): adult worms with clitellum
- Weight at test initiation (mean and range, SD): 300 - 600 mg
ACCLIMATION
- Acclimation period: at least 24 h prior to the start of the test
- Acclimation conditions: same as test - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 19 - 21 °C
- pH:
- 6.1 - 6.4
- Moisture:
- 33.0 - 34.7% of dry mass
56.1 - 59.1% of WHCmax - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1.5 L preservation jars covered with glass lids
- Amount of soil or substrate: 500 g soil dw
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4
SOURCE AND PROPERTIES OF SUBSTRATE
- Composition of the artificial substrate):
- 10% Sphagnum peat
- 20% Kaolin-Clay (kaolinite content > 30%
- 68-69% Quartz sand
- ca. 1% Calcium carbonate (CaCO3)
OTHER TEST CONDITIONS
- Photoperiod: permanent
- Light intensity: 475-577 lx
EFFECT PARAMETERS MEASURED: mortality and biomass determined after 7 and 14 days
VEHICLE CONTROL PERFORMED: yes - Nominal and measured concentrations:
- Nominal test concentrations: control, 62.5, 125, 250, 500, 1000 mg/kg soil dw
- Reference substance (positive control):
- yes
- Remarks:
- cloroacetamide
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- weight
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 198.2 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: 2.5% mortality was observed at the control and the solvent control, and 0-2.5% mortality at the concentrations of 62.5 and 125.0 mg test item/kg soil dw. At 250 mg test item/kg soil dw 95% mortality was determined. At 500 and 1000 mg test item/kg 100% mortality was observed.
- Total mass of adults at beginning of test: No significant difference between the solvent control and the water control was observed. A significant difference after 14 days between the solvent control and the concentration of 250 mg test item/kg soil dw was determined.
- Morphological abnormalities: none
- Behavioural abnormalities: none - Results with reference substance (positive control):
- LC50 (14 d) = 28.2 mg/kg soil dw
- Validity criteria fulfilled:
- yes
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to analogue justification provided in IUCLID section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- weight
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 198.2 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Referenceopen allclose all
Description of key information
NOEC (14 d): 125 mg/kg dw
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 125 mg/kg soil dw
Additional information
Data on the toxicity of sodium 2-biphenylate (CAS 132-27-4) soil macroorganisms are not available. The assessment was therefore based on a study conducted with the source substance 2-phenylphenol (CAS 90-43-7) as part of a read across approach, which is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Further justification is given within the overall summary chapter 6.3 and within the analogue justification in IUCLID section 13.
The effects of 2-phenylphenol (CAS 90-43-7) on earthworms were studied in an acute toxicity test on Eisenia fetida (Moser & Scheffczyk, 2004). This GLP study was carried out according to OECD Guideline 207 in artificial soil. In this test individuals of Eisenia fetida were exposed to nominal test substance concentrations of 62.5, 125, 250, 500, 1000 mg/kg soil dw for 14 days. The mortality of test animals was determined after 7 and 14 days of exposure. At test termination a LC50 (14 d) of 198.2 mg/kg soil dw (nominal) was determined. The NOEC for mortality as well as the NOEC for biomass was determined to be 125 mg test item/kg dw. Other effects on the morphology and the behaviour of the worms were not observed.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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