Saccharomyces cerevisiae, lysate

Oxidising properties

Link to relevant study record(s)

Reference

Endpoint:

oxidising solids

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 05/02/2018 to 06/03/2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.17 (Oxidising Properties (Solids))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Contact with:

powdered cellulose

Key result

Sample tested:

reference mixture as specified

Remarks:

Composition of 2:1 ratio (barium nitrate : powdered cellulose

Parameter:

preliminary test

Result:

41 s

Remarks on result:

vigorous reaction in the preliminary test

Remarks:

Orange, sparkling flame

Key result

Sample tested:

reference mixture as specified

Remarks:

Composition of 2:1 ratio (Test item : powdered Cellulose)

Parameter:

preliminary test

Remarks on result:

other: Orange flame which extinguished within 4 seconds

 Drying of sample

TrayNo.	Tray weight (g)	Tray & wet powder weight (g)	Tray & dry powder weight (g)	Weight of sample before drying (g)	Weight of sample after drying (g)	Moisture loss (g)	Moisture loss (%)
Saccharomyces Cerevisiae, Lysate dried at 105°C for approximately 15 hours
1	476.3	602.9	597.5	126.7	121.2	5.4	4.3
2	390.8	528.8	522.6	138.1	131.8	6.2	4.5
3	460.0	693.3	683.1	233.3	223.1	10.3	4.4
Cellulose dried at 105°C for approximately 91 hours
1	452.8	554.0	550.5	101.2	97.7	3.5	3.5
2	390.6	447.2	445.1	56.6	54.5	2.1	3.7
Barium nitrate dried at 105°C for approximately 3 hours
1	476.4	822.5	821.4	346.0	345.0	1.1	0.3

 

Preliminary Screening Test

Composition of 2:1 Ratio	Mass of material (g)	Type Reference sample	Burning time (sec)	Comments on burning behaviour
Reference sample	6	Vigorous	41	Orange, sparkling flame
Cellulose	3
Saccharomyces Cerevisiae,Lysate	6	Moderate	N/A	Orange flame which extinguished within 4 seconds
Cellulose	3

 

 

Interpretation of results:

GHS criteria not met

Conclusions:

The reaction for the Saccharomyces Cerevisiae, Lysate/ cellulose mixture was significantly slower than the reference mixture. It is clear, therefore, that the sample is not an oxidising solid.

Executive summary:

The oxidizing properties of Saccharomyces cerevisiae has been tested according to EU Method A17 (solids) and the GLP.

After being dried at 105°C until constant weight and the particule size reduced to < 0.125 mm, the test item was mixed with cellulose in the proportion 2 of test substance to 1 of cellulose. This mixture forming a small cone was put in contact with a hot flame. The vigour and duration of the reaction was observed in comparison with the behaviour of the reference substance (barium nitrate) mixed with cellulose and subjected to the same conditions. The reaction for the Saccharomyces Cerevisiae, Lysate/ cellulose mixture was significantly slower than the reference mixture. It is clear, therefore, that the sample is not an oxidising solid.

This oxidizing properties study is classified as acceptable, and satisfies the guideline requirements of the EU method A17

Description of key information

The reaction for the test item / cellulose mixture was significantly slower than the reference mixture. It is clear, that Saccharomyces Cerevisiae, Lysate is not an oxidising solid.

Key value for chemical safety assessment

Oxidising properties:

non oxidising

Additional information

The sample has been tested according to EU Method A17 (solids) and the GLP.

After being dried at 105°C until constant weight and the particule size reduced to < 0.125 mm, the test item was mixed with cellulose in the proportion 2 of test substance to 1of cellulose. This mixture forming a small cone was put in contact with a hot flame. The vigour and duration of the reaction was observed in comparison with the behaviour of the reference substance (barium nitrat) mixed with cellulose and subjected to the same conditions. The reaction for the Saccharomyces Cerevisiae, Lysate/ cellulose mixture was significantly slower than the reference mixture. It is clear, therefore, that the sample is not an oxidising solid.