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EC number: 278-031-6
CAS number: 74931-55-8
in vitro skin corrosion study was performed for 2-ethylhexanoic
acid, compound with 2-aminoethanol (1:1) in line with OECD Guideline 431
(In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test
Method). The test was compliant with GLP. 2-Ethylhexanoic acid, compound
with 2-aminoethanol (1:1) was applied neat at 50µl to reconstructed
human epidermal model Epiderm™. A negative (sterile water) and positive
control (potassium hydroxide) was prepared and run in parallel. Exposure
was permitted for a 3- and 60-minute period at 37 °C, 5 % CO2, 95 % RH.
Corrosion is directly related to cytotoxicity in the EpiDerm™ tissue.
Cytotoxicity is determined by the reduction of MTT
(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) to
formazan by viable cells in the test item treated tissues relative to
the corresponding negative control. The results are then used to make a
prediction of the corrosivity potential of the test item.
controls were valid and demonstrated the reliability of the test system.
Mean tissue viability, calculated as a percentage of the negative
control, was 8.6 % after a 60-minute exposure and 97.6 % after a
3-minute exposure. A classification for skin corrosion (Skin Corr.
1B/1C) is subsequently required for 2-ethylhexanoic acid, compound with
2-aminoethanol (1:1) according to CLP Regulation (EC) No. 1272/2008.
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