Reaction Mass of (E,E)-2,6-dimethylocta-2,4,6-triene and (E,Z)-2,6-dimethylocta-2,4,6-triene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Reference 2

Reference Type:

other: Monograph

Title:

2,6-Dimethyl-2,4,6-Octatriene

Year:

1988

Bibliographic source:

Food and Chemical Toxicology 26 (4), 309

Materials and methods

Principles of method if other than guideline:

- Principle of test: Acute oral toxicity in Wistar rats
- Short description of test conditions: Ten male Wistar rats were used with initial body weights of 200-287 grams. Mortality and/or systemic effects were recorded 3-4 hours after dosing and daily thereafter for 14 days. Gross necropsy was carried out on all animals. LD50 was calculated by the method of Litchield and Wilcoxon.
- Parameters analysed / observed: LD50, mortality, systemic effects, gross necropsy

GLP compliance:

no

Test type:

other: Acute oral toxicity in Wistar rats

Limit test:

no

Test material

Test material information

1

Reference substance name:

(E,E)-2,6-dimethylocta-2,4,6-triene

EC Number:

221-153-1

EC Name:

(E,E)-2,6-dimethylocta-2,4,6-triene

Cas Number:

3016-19-1

Molecular formula:

C10H16

IUPAC Name:

(E,E)-2,6-dimethylocta-2,4,6-triene

Test material form:

liquid

Specific details on test material used for the study:

The test material (E,E)-2,6-dimethylocta-2,4,6-triene (EC 221-153-1) is the constituent of the registered substance.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

Initial body weight of rats: 200-287 g

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

unchanged (no vehicle)

Doses:

1200, 3100 and 5000 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 1 900 mg/kg bw

Based on:

test mat.

95% CL:

>= 1 600 - <= 2 300

Remarks on result:

other: Calculation method: Litchield and Wilcoxon

Effect levels 2

Sex:

male

Dose descriptor:

LD0

Effect level:

ca. 1 200 mg/kg bw

Based on:

test mat.

Effect levels 3

Sex:

male

Dose descriptor:

LD100

Effect level:

>= 3 200 mg/kg bw

Based on:

test mat.

Effect levels 4

Sex:

male

Dose descriptor:

LD100

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

1200 mg/kg bw: 0/10 deaths
1900 mg/kg bw: 5/10 deaths
3100 mg/kg bw: 10/10 deaths
5000 mg/kg bw: 10/10 deaths.

Clinical signs:

other: 1200 mg/kg bw: chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid 1900 mg/kg bw: chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid 3100 mg/kg bw: lethal - chromorhinorrhea, piloer

Gross pathology:

All dead animals had abnormalities in lungs, heart, kidneys, stomach, testes and/or intestine at necropsy.

Applicant's summary and conclusion

Conclusions:

The acute oral LD50 of 2,6-dimethyl-2,4,6-octatriene (CAS 3016-19-1) in Wistar male rats is 1900 mg/kg bw (95% C.I. 1600-2300 mg/kg body weight). Clinical signs observed were chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid. Necropsy of dead animals revealed abnormalities in lungs, heart, kidneys, stomach, testes and/or intestines.

Executive summary:

The acute oral LD50 of 2,6-dimethyl-2,4,6-octatriene (CAS 3016-19-1) in Wistar male rats is 1900 mg/kg bw (95% C.I. 1600-2300 mg/kg body weight). Clinical signs observed were chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid. Necropsy of dead animals revealed abnormalities in lungs, heart, kidneys, stomach, testes and/or intestines.