Phosphoric acid, 2-ethylhexyl ester, compd. with 2,2'-iminobis[ethanol]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23/01/2006 - 16/03/2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: ISO 14669:1999 (E) - Draft method

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on sampling:

- Concentrations: Rangefinding concentrations: 0, 2.0, 20.0, 200.0 mg/L test material, 0, 20.0, 35.6, 63.3, 112.5, 200.0 mg/L
- Sampling method: Percentage immobilisation in population

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test material dissolved in seawater (1 g/ L) and aliquots taken from stock to make range of lower concentrations
- Controls: 1 mortality control (0 mg/L test material) and 1 positive control (reference substance - 3, 5 - dichlorophenol) for each time point (24 and 48 hours).
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No, test substance was soluble in seawater for maximum concentration tested.

Test organisms (species):

other aquatic crustacea: Acartia tonsa

Details on test organisms:

TEST ORGANISM
- Common name: Acartia tonsa
- Batch number: AT06009
- Source: Guernsey Sea Farms, Guernsey
- Age of parental stock: Unknown, test organisms used were 13 - 15 days olds.

Test type:

semi-static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

Measurements taken at 24 and 48 hours

Test temperature:

Test condition not provided.
Seawater on arrival was 12 °C and it was stored at 20 (+-2) °C prior to use

pH:

Test condition not provided.
Seawater on arrival was pH 7.99

Dissolved oxygen:

Test condition not provided.
Seawater on arrival had a 100 % dissolved oxygen level.

Salinity:

Test condition not provided.
Seawater salinity was 33.2 g/L on arrival.

Nominal and measured concentrations:

Nominal test substance concentrations, 0, 20, 35.6, 63.3, 112.5, 200.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel volume: At least 5 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Guernsey Sea Farms
- Alkalinity: pH 7.99 (on arrival)
- Salinity: 33.2 g/L (on arrival)
- Intervals of water quality measurement: at beginning and end of the test, not stated in the study

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality for 24 and 48 hours

RANGE-FINDING STUDY
- Test concentrations: 0, 2.0, 20.0, 200.0 mg/L test material
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

120.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

70.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

63.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

112.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? Information not provided
- Relevant effect levels: LC50, NOEC and LOEC
- Limit test: Yes, only 1.0 mg/L tested

Validity criteria fulfilled:

yes

Conclusions:

The LC50 for 48 hours was 70.2 mg/L and for 24 hours it was 120.5 mg/L. The NOEC for 48 hours was 63.3 mg/L and the LOEC for 48 hours was 112.5 mg/L

Executive summary:

This study assesses the actue toxicity for 25% M-528 -7 (W/W) neutralised with DEA in deionised water for marine invertebrates. 25% M-528 -7 (W/W) was identified as the registered substance. The study is performed in compliance with GLP and according to the method ISO 14669:1999 guideline. All validity criteria were met. The test organism used was Acartia tonsa and the test was performed in saltwater (seawater). A rangefinding test was performed using the concentrations 0, 2.0, 20.0 and 200.0 mg/L to determine a definitive test range. The definitive test used a range of nominal concentrations 0, 20.0, 35.6, 63.3, 112.5 and 200.0 mg/L test substance. This range was produced by dissolving the test material in seawater (1.0 g/ L) from which aliquots were taken to make dilutions. The results of the definitive test were LC50 for 24 hours was 120.5 mg/L and for 48 hours was 70.2 mg/L, the NOEC for 48 hours was 63.3 mg/L and the LOEC for 48 hours was 112.5 mg/L. The definitive test contained data points for 24 and 48 hours per concentration, with one replicate each. Two mortality controls (0 mg/L test substance) were included per time period with 0% and 3% immobilisation/mortality at 24 and 48 hours, respectively. Two positive controls were included (reference substance, 3,5 - dichlorophenol at 1mg/L) per time period with 0% and 55% immobilisation at 24 and 48 hours, respectively

Description of key information

This study assesses the acute toxicity for 25% M-528 -7 (W/W) neutralised with DEA in deionised water for marine invertebrates. 25% M-528 -7 (W/W) was identified as the registered substance, The study is performed in compliance with GLP and according to the method ISO 14669:1999 guideline. All validity criteria were met. The test organism used was Acartia tonsa and the test was performed in saltwater (seawater). A rangefinding test was performed using the concentrations 0, 2.0, 20.0 and 200.0 mg/L to determine a definitive test range. The definitive test used a range of nominal concentrations 0, 20.0, 35.6, 63.3, 112.5 and 200.0 mg/L test substance. This range was produced by dissolving the test material in seawater (1.0 g/ L) from which aliquots were taken to make dilutions. The results of the definitive test were LC50 for 24 hours was 120.5 mg/L and for 48 hours was 70.2 mg/L, the NOEC for 48 hours was 63.3 mg/L and the LOEC for 48 hours was 112.5 mg/L. The definitive test contained data points for 24 and 48 hours per concentration, with one replicate each. Two mortality controls (0 mg/L test substance) were included per time period with 0% and 3% immobilisation/mortality at 24 and 48 hours, respectively. Two positive controls were included (reference substance, 3,5 - dichlorophenol at 1mg/L) per time period with 0% and 55% immobilisation at 24 and 48 hours, respectively

Key value for chemical safety assessment

Marine water invertebrates

Marine water invertebrates

Effect concentration:

70.2 mg/L