Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 277-274-5 | CAS number: 73070-48-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23/01/2006 - 30/03/2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: Nominal test substance concentrations used were 0, 10.0, 17.8, 31.6, 56.2 and 100 mg/ L
- Sampling method: Growth rate observations over time. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test material dissolved in seawater
- Controls: 1 growth rate control (for 0 hours 1 replicate, for 24, 48 and 72 hours 6 replicates per concentration). 1 positive control (3,5-dichlorophenol, 1.5 mg/ L for 24, 48 and 72 hours with 3 replicates.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No, test material was soluble in water. - Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- TEST ORGANISM
- Common name: Skeletonema costatum
- Strain: CCAP1077/5
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa, Dunstaffnage.
- Method of cultivation: Guillard's f/2 medium + Si
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- Measurement time points were 0, 24, 48, 72 hours.
- Test temperature:
- 19 - 21 °C
- pH:
- At 0 hours, pH was 8.09 - 8.19
At 72 hours, pH was 8.49 - 8.51 - Nominal and measured concentrations:
- Nominal test substance concentrations - 0, 10.0, 17.8, 31.6, 56.2 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: culture flasks
- Initial cells density: 2520 cells per mL
- Control end cells density: 16478 - 229872 cells per mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): Growth rate control (6), positive control (3)
GROWTH MEDIUM
- Detailed composition if non-standard medium was used: Guillard's f2 medium + Si
TEST MEDIUM / WATER PARAMETERS
- Alkalinity: pH 8.09 - 8.19
- Intervals of water quality measurement: 24 hour intervals
OTHER TEST CONDITIONS
- Photoperiod: continuous for 72 hours
- Light intensity and quality: 6000 - 10000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Cell number changes over time
- Determination of cell concentrations: microscopic counting
TEST CONCENTRATIONS
- Test concentrations: 0, 10, 17.8, 31.6, 56.2 and 100.0 mg/L
- Results used to determine the conditions for the definitive study: information from a previous study - Reference substance (positive control):
- yes
- Remarks:
- 3, 5 - dichlorophenol
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): No
- Unusual cell shape: No
- Colour differences: No
- Any stimulation of growth found in any treatment: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance has an EC50 value of 5.77 mg/ L for 24 hours, 11.6 mg/L for 48 hours and 16.8 mg/ L for 72 hours. The NOEC value for 72 hours is 10.0 mg/ L and the LOEC value is 17.8 mg/ L.
- Executive summary:
The study assesses the acute toxicity of 25% M528 -7 (W/W) neutralised with DEA in deionised water to marine algae. M528 -7 (W/W) was identified as the registered substance.
The study is in compliance with GLP and the testing guidelines complied with EN ISO 10253:1998. All validity criteria were met. The organism used was Skeletonema costatum in saltwater (seawater). A rangefinding test was not used as the information was obtained from a previous screening test performed by the laboratory. The definitive test used a range of nominal concentrations of 0, 10, 17.8, 31.6, 56.2 and 100 mg/ L of test substance. Test material was dissolved at 25% with DEA in deionised water to make a 1 g/ L stock from which aliquots were used for dilutions. The results of the definitive test showed that the test substance has an EC50 of 5.77 mg/ L for 24 hours, 11.6 mg/L for 48 hours and 16.8 mg/ L for 72 hours. The NOEC value for 72 hours is 10.0 mg/ L and the LOEC value is 17.8 mg/ L. The definitive test had 3 replicates per concentration, per time point. 1 growth rate control was included (0 mg/ L test substance) for all time points, with 1 replicate for 0 hours and 6 replicates for 24, 48 and 72 hours. One positive control (reference substance: 3, 5 -dichlorophenol) was included with 3 replicates per time point.
Reference
Description of key information
The study assesses the acute toxicity of 25% M528 -7 (W/W) neutralised with DEA in deionised water to marine algae. M528 -7 (W/W) was identified as the registered substance.
The study is in compliance with GLP and the testing guidelines complied with EN ISO 10253:1998. All validity criteria were met. The organism used was Skeletonema costatum in saltwater (seawater). A rangefinding test was not used as the information was obtained from a previous screening test performed by the laboratory. The definitive test used a range of nominal concentrations of 0, 10, 17.8, 31.6, 56.2 and 100 mg/ L of test substance. Test material was dissolved at 25% with DEA in deionised water to make a 1 g/ L stock from which aliquots were used for dilutions. The results of the definitive test showed that the test substance has an EC50 of 5.77 mg/ L for 24 hours, 11.6 mg/L for 48 hours and 16.8 mg/ L for 72 hours. The NOEC value for 72 hours is 10.0 mg/ L and the LOEC value is 17.8 mg/ L. The definitive test had 3 replicates per concentration, per time point. 1 growth rate control was included (0 mg/ L test substance) for all time points, with 1 replicate for 0 hours and 6 replicates for 24, 48 and 72 hours. One positive control (reference substance: 3, 5 -dichlorophenol) was included with 3 replicates per time point.
Key value for chemical safety assessment
- EC50 for marine water algae:
- 16.8 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
