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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study without analytical monitoring, but study is well documented. This is only a minor shortcoming and do not affect the overall significance of the findings.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted April 1981
Deviations:
yes
Remarks:
Test duration extended to 48 hours (which was optional, according to OECD guideline 202 adopted April 1981). However, this duration is usual today.
GLP compliance:
no
Remarks:
GLP was not compulsory at the time the study was performed (1982). Experimental procedures and study report were reviewed by the laboratory’s quality assurance unit
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Name of test material in study report: Magnesium-monoperoxyphthalate-hexahydrate, H48

OTHER SPECIFICS:
white powder
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: no data
- Age of parental stock: < 24 h at test initiation
- Feeding during test: no

ACCLIMATION
- Type and amount of food: Suspension of trout chow and alfalfa (PR-11) supplemented with algae (Selenastrum capricornutum), amount not stated
- Feeding frequency: At least every three days prior to testing
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
255 mg/L as CaCO3
Test temperature:
20 °C +- 2.0 °C
pH:
7.8 – 8.6
Dissolved oxygen:
7.5 - 8.6 mg/L
Conductivity:
50 µmhos/cm
Nominal and measured concentrations:
Initial concentrations: control, 10, 18, 32, 56 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL beakers, containing 200 mL medium (well water)
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water at ABC's Aquatic Bioassay Laboratory
- Alkalinity: 368 mg/L as CaCO3
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 hours light:dark
- Light intensity: 50 - 70 footcandles (One footcandle is approximately 10.76 lux).

EFFECT PARAMETERS MEASURED:
Immobility, sampled after 24 and 48 hours
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
42 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
Calculation of the median effective concentration causing 50% effect (EC50) by binominal test

Effect data (Immobilisation)

Test substance
Concentration
(nominal)

[mg/l]

 

ImmobileDaphnia

 

Number

Percentage

24 h

48 h

24 h

48 h

Control A

0

0

0

0

 

Control B

0

0

0

0

 

10

0

0

0

0

 

18

0

0

0

0

 

32

0

0

0

0

 

56

0

10

0

100

 

100

10

10

100

100

 

 

 

 Results

 

EC50

95 % c.l.

EC0

EC100

24 h [mg/l] (nominal)

75

56 -100

56

100

48 h [mg/l](nominal)

42

32 - 56

32

56

 

 

Validity criteria fulfilled:
yes
Conclusions:
The test results indicate that MMPP is acutely harmful towards Daphnia.
Executive summary:

The acute toxicity of MMPP towards invertebrates was performed according to the OECD guideline 202 (1981). The water flea Daphnia magna STRAUS (Crustacea: Cladocera) was used.

Animals were exposed to several of H48 concentrations, ranging from 10 to 100 mg/L. The test was performed at 20.0°C and a 16:8 light:dark cycle in well water. Immobilisation was assessed at 24 and 48 hours, physico-chemical water parameters were measured at 0 and 48 hours.

Analytical monitoring of test concentration was not performed. Volatility and adsorption of MMPP from aqueous solutions can be neglected and therefore these properties have no influence on the result. However, MMPP hydrolyses in aqueous solution and MMPP is regarded as readily biodegradable; both effects may reduce the nominal concentration. Nevertheless, an analytical monitoring is not necessary, as the aqueous solution of MMPP reflects the emission of hydrolyses products to aquatic organisms. Furthermore, the biodegradability is assumed to have only minor effects on the results due to the relatively short test duration. The concentration in aqueous solution is assumed to be sufficiently stable under the test conditions due to the analytical results shown in the algae growth inhibition test.All other validity criteria according the test guidline OECD 202 are met within the study

Description of key information

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
42 mg/L

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