Phenol, 4-isocyanato-, 1,1',1''-phosphorothioate, reaction p roducts with 3-(trimethoxysilyl)-N-(3-(trimethoxysilyl)propy l)-1-propanamine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

Following a preliminary range-finding test, Desmodesmus subspicatus was
exposed to Water Accommodated Fractions (WAFS) of the test material over a range of
nominal loading rates of 10, 20, 40, 80 and 160 mg/l (three replicate flasks per
concentration) for 72 hours, under constant illumination and shaking at a temperature of 24
± 1°C.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

> 160 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

TOC analysis of the test preparations was performed at 0 and 72 hours. Analysis of the
O-Hour test samples showed measured test concentrations to range from 3.69 to
21.88 mg CII whilst concentrations in the range of less than the control to 4.35 mg CII
were obtained at 72 hours.
As the test material is a complex mixture, the toxicity cannot be attributed to anyone or
more of the components of the test material but to the test material as a whole.
Therefore the results were based on nominal loading rates only.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The effect of the test material on the growth of Desmodesmus subspicatus has been
investigated and gave EL*so values of greater than 160 mg/l loading rate WAF.
Correspondingly the No Observed Effect Loading Rate was 160 mg/lloading rate WAF.