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EC number: 947-930-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3/10/2017 to 31/10/2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD Chemicals Testing Guideline No.202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tris(2-ethylhexyl) 2-hydroxypropane-1,2,3-tricarboxylate
- EC Number:
- 230-457-3
- EC Name:
- Tris(2-ethylhexyl) 2-hydroxypropane-1,2,3-tricarboxylate
- Cas Number:
- 7147-34-4
- Molecular formula:
- C30H56O7
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Test Substance Name: Bernel Ester TCC
Chemical Name: Tris(2-ethylhexyl) 2-hydroxypropane-1,2,3-tricarboxylate
CAS Number: 7147-34-4
Batch Number: P7803
Purity: 94%
Receipt Date: 12 June 2017
Expiry Date: 23 January 2019
Storage on Receipt: Room temperature (15 - 30°C)
Test substance details were supplied by the Sponsor. The Certificate of Analysis is presented in Appendix 4 (attached).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Juvenile Daphnia magna (Straus), less than 24 hours old, were taken from healthy parental laboratory cultures, held and maintained at the test facility. The culture was originally obtained from Microbiotest, Belgium. Details of the Daphnia magna culture maintenance are presented in Appendix 1 (attached).
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- Control 19.2 °C (0 hours) to 18.6 °C (48 hours)
100 19.4 °C (0 hours) to 18.6 °C (48 hours) - pH:
- Control 7.80 (0 hours) to 7.76 (48 hours)
100 7.87 (0 hours) to 7.84 (48 hours) - Dissolved oxygen:
- Control 8.40 mg/l (0 hours) to 9.38 mg/l (48 hours)
100 8.49 mg/l (0 hours) to 9.34 mg/l (48 hours) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate, Batch No. 16F014128, Purity: 100%, expiry date: 31 Jan 2021
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.185 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.185 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
Range-finding Test
The results of the range-finding test are summarised below:
Nominal concentration (% saturated solution) |
Cumulative immobilisedDaphnia magna* | ||||
24 hours | 48 hours | % immobility | |||
R1 | R2 | R1 | R2 | ||
Control | 0 | 0 | 0 | 0 | 0 |
1 | 0 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | 0 | 0 | 0 |
100 | 0 | 0 | 0 | 0 | 0 |
* Initial population of Daphnia magna: five per replicate
R Replicate
Chemical analysis was not conducted during the range-finding test.
The results of the range-finding test suggested that toxicity would not be observed at the highest concentration of 100% saturated solution.
Media Trial
Media trial results are summarised below:
Nominal concentration (% saturated solution) |
Measured concentrations (mg/l) | |
0 hours | 48 hours | |
100 | 0.389* | 0.144 |
LOQ (limit of quantification) = 0.01 mg/L
* Initial batch failed to meet acceptance criteria, due to results being above the calibration line. Samples re-run with 48 hour samples.
Results showed a decline in concentration over the 48-hour test period. Therefore, the definitive phase was run semi-static.
Definitive Test
Chemical Analysis
The results of the chemical analysis are presented in Table 1 (attached).
Example chromatograms of test samples, standard solution and a typical calibration line are presented in Appendix 2 (attached).
The limit of quantification (LOQ) for Bernel Ester TCC in Elendt M4 medium using this method was 0.01 mg/L.
Analysis of the test media samples was conducted on fresh media at 0 and 24 hours, analysis of the corresponding old media was conducted at 24 and 48 hours.
Chemical analysis showed concentrations of Bernel Ester TCC declined over each 24-hour renewal period. Therefore, results were based on time-weighted mean measured concentrations. This was calculated to be 0.185 mg/L
Water Quality
Water quality determinations for pH, temperature and dissolved oxygen (% ASV and mg/L) during the definitive test are presented in Table 2 (attached).
Test Media Descriptions
The test preparations were observed to be colourless solutions throughout the duration of the test.
Toxicity to Daphnia magna
The numbers of immobilised Daphnia magna at 24 and 48 hours, following exposure are presented in Table 3 and Table 4 (attached). The percentage immobility data is summarised below.
Nominal concentration (% saturated solution) |
Cumulative immobilisedDaphnia magna* (initial population: 5Daphniaper replicate) | |||||||||
24 hours | 48 hours | |||||||||
R1 | R2 | R3 | R4 | % immobility | R1 | R2 | R3 | R4 | % immobility | |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R Replicate
* All daphnia in 100% saturated solution test group were observed to be at the surface of the test media at 48 hours, one daphnia in the 100% saturated solution test group was observed to be at the surface of the test media at 24 hours.
No significant toxicity (>10%) was observed during the test. Due to the decline in test substance concentration observed over each renewal period results were based on time-weighted mean measured concentration. This was calculated to be 0.185 mg/L.
Statistical Analysis
The 3EC50 values, the corresponding 4NOEC and 3LOEC values are presented in the following table.
Nominal concentration (mg/l) | Statistical test | ||
24 hour | 48 hour | ||
EC50 | >0.185 mg/l | >0.185 mg/l | |
NOEC | 0.185 mg/l | 0.185 mg/l | Derived Empirically |
LOEC | >0.185 mg/l | >0.185 mg/l |
3 Concentration resulting in 50% immobility of exposed Daphnia magna
4 No observed effect concentration
3 Lowest observed effect concentration
Based on time-weighted mean measured concentrations, the 48-hour EC50 was calculated to be >0.185 mg/L.
The corresponding NOEC values was calculated to be >0.185 mg/L.
The validity criteria for control immobility (≤10%) and dissolved oxygen (≥3 mg/L) were both satisfied. The test is therefore considered valid.
Validity Criteria
The validity criteria for control immobility (≤10%) and dissolved oxygen (≥3 mg/L) were both satisfied. The test is therefore considered valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour acute toxicity of Bernel Ester TCC to the freshwater planktonic crustacean, Daphnia magna, was determined in accordance with the requirements of OECD Chemicals Testing Guideline No. 202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004).
The validity criteria for control immobility (≤10%) and dissolved oxygen (≥3 mg/L) were both satisfied. The test is therefore considered valid.
Based on time-weighted mean measured concentration, the 48-hour EC50 value was determined to be >0.185 mg/L. The corresponding No Observed Effect Concentration (NOEC) was considered to be 0.185 mg/L. - Executive summary:
The objective of the study was to determine the 48-hour acute toxicity of Bernel Ester TCC against the mobility of the freshwater planktonic crustacean, Daphnia magna.
The study was conducted in accordance with the requirements of OECD Chemicals Testing Guideline No. 202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004).
Based on the results of a range-finding test, for which the key results only have been reported, a definitive limit test was conducted at a single concertation of 100% saturated solution. A control group was also included. The test was conducted under semi-static test conditions with media preparation at 0 and 24 hours.
At the start of the test, five juvenile Daphnia magna were added to each test vessel. The Daphnia magna in each test vessel were observed at 24 and 48 hours. The Daphnia magna were not fed during the test.
Chemical analysis of the 100% saturated solution test concentration and control was conducted on fresh media at 0 and 24 hours, analysis was conducted on the corresponding old media at 24 and 48 hours. Analysis of the test samples at 0, 24 and 48 hours are summarised in the table below.
Nominal
concentration
(% saturated
solution)Measured concentration (mg/l) 0 hours (New) 24 hours (Old) 24 hours (New) 48 hours (Old) R1 R2 R1 R2 Control - - - - 100 0.183 0.0673 0.341 0.183 - None found above LOQ (0.01 mg/L)
24-hour renewal period. Therefore, results were based on time-weighted mean measured concentrations. This was calculated to be 0.185 mg/L.
The1EC50values, the corresponding 2NOEC and 3LOEC values are presented in the following table.
Nominal concentration (mg/l) Statistical test 24 hour 48 hour EC50 >0.185 mg/l >0.185 mg/l NOEC 0.185 mg/l 0.185 mg/l Derived Empirically LOEC >0.185 mg/l >0.185 mg/l 1Concentration resulting in 50% immobility of exposed Daphnia magna
2No observed effect concentration
3Lowest observed effect concentration
Based on time-weighted mean measured concentrations, the 48-hour EC50 was calculated to be >0.185 mg/L.
The corresponding NOEC values was calculated to be >0.185 mg/L.
The validity criteria for control immobility (≤10%) and dissolved oxygen (≥3 mg/L) were both satisfied. The test is therefore considered valid.
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