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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication/study report which meets basic scientific principles

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1986
Reference Type:
secondary source
Title:
1-CHLORO-4-NITROBENZENE CAS No: 100-00-5 SIDS Initial Assessment Report.
Author:
OECD
Year:
2002
Bibliographic source:
UNEP Publications

Materials and methods

Principles of method if other than guideline:
NEN 6502: Determination of chronic toxicity to Daphnia magna (1980)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-4-nitrobenzene
EC Number:
202-809-6
EC Name:
1-chloro-4-nitrobenzene
Cas Number:
100-00-5
Molecular formula:
C6H4ClNO2
IUPAC Name:
1-chloro-4-nitrobenzene
Details on test material:
- Name of test material (as cited in study report): 4-chloronitrobenzene
- Analytical purity: > 98 %

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The daphnids were fed with 3.0E+08 cells/L Chorella pyrenoidosa

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
21 d

Test conditions

Hardness:
200 mg/L CaCO3
Test temperature:
20 °C
pH:
8.4
Details on test conditions:
- The population growth constant (Rm) of Daphnia magna was determined under semistatic conditions over a 21 days period, using 10 daphnids per concentration and one animal per jar containing 10 mL medium.
- The test was carried out in a room illuminated 12h/day.

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
4.6 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Remarks on result:
other: 95% CL; 4.3-5.0
Duration:
21 d
Dose descriptor:
other: LRCT rm
Effect conc.:
1.8 mg/L
Nominal / measured:
nominal
Basis for effect:
other: population growth
Remarks on result:
other: LRCT = Lowest rejected concentration tested with effect (similar to LOEC); rm=intrinsic rate of natural increase
Duration:
21 d
Dose descriptor:
other: LRCT L
Effect conc.:
3.2 mg/L
Basis for effect:
growth
Remarks on result:
other: = Lowest rejected concentration tested with effect (similar to LOEC); L=mean length (L) of animals

Any other information on results incl. tables

Three chronic effects are presented.
1. Population growth:
LRCT (Rm)= Lowest rejected concentration tested which
significantly lowered the population growth constant (Rm)
after 21 days of exposure:
log LRCT(Rm) = 1.05 = 11 µmol/L.....LRCT (Rm)=1.8 mg/L

2. Body length:

LRCT(L) = Lowest rejected concentration tested that significantly lowered the mean length (L) of animals after 21 days of exposure. log LRCT(L) = 1.31 = 20 µmol/L....LRCT(L)= 3.2 mg/L

3. Lethal concentration:

LC50 (21 days) = 4.6 (95 % confidence limits: 4.3 - 5.0) mg/L In another publication, Deneer et al. (Deneer, J.W. et al., QSAR study of the toxicity of nitrobenzene derivatives towards Daphnia magna, Chlorella pyrenoidosa and Photobacterium phosphoreum, Aquatic Toxicology 15, 83-98 (1989)), two of the authors presented the first two chronic effects but reported the third to be IC50 = 21 d immobilization concentration log IC50 = 1.46 = 28 µmol/L .......IC50 = 4.5 mg/L

Applicant's summary and conclusion