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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
07/05/1997 to 21/05/1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other:
Version / remarks:
92/69/EEC
Principles of method if other than guideline:
Short term toxicity test exposing Daphnia magna to the test substance and observing the occurrence of immobilisation of the time frame in order to calculate an EC50.
GLP compliance:
yes
Specific details on test material used for the study:
Common name of substance: MIGLYOL 8810
CAS number; not specified
Purity: C: 71.08%, H 11.57% 97.9% area % 1,3-butanediol ester
Stability: >1 year
Appearance: clear liquid
Batch number: 704 025
Date of manufacture: 03/04/1997
Analytical monitoring:
yes
Details on sampling:
Initial testing for determining stability of the test substance:
- Concentrations: 2.4, 1.2 , 0.5 mg/L
- Analysis: carbon content using the TOC analyser
- Sampling times: 24, 48 and 72 hours
- Recovery rate: 80% (samples at some times points are lower and the 0.5mg/L is below detection limit at 24, 48 and 72 hours)
See Table 1 for results
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock was prepared at 1g/L in synthetic freshwater. The stock was stirred for 18 hours and then filtered. The carbon content was then analysed.
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not stated
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain/clone: Staus, clone 5
- Source: Laboratory own breed
- Age of parental stock (mean and range, SD): Not stated
- Feeding during test: No
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable

CULTURE CONDITIONS
- Culture conditions (same as test or not):M4 medium (1990).
- Type and amount of food: Scenedesmus subspicatus
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not stated
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
not stated
Test temperature:
20 ± 1 ° C
pH:
7.7 to 8.1 at end of test
Dissolved oxygen:
not stated
Salinity:
not stated
Conductivity:
not stated
Nominal and measured concentrations:
Nominal - 2mg/L (demonstrated in stability experiment that measured were within 20% of nominal), control
Details on test conditions:
Composition of synthetic freshwater: CaCl2 x H2O - 294mg/L, MgSO4 x 7 H2O - 123mg/L, NaHCO3 - 63 mg/L, KCl 5.5 mg/L.
Test set up: roud arch glasses 10ml
Number of test concentrations: 1 exposure + control
Number of replicates: 4
Number of animals in each: 5
Stocking density: 2ml per test organism
Light profile: dark
Aeration: none
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 2 mg/L
Nominal / measured:
nominal
Conc. based on:
other: carbon
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 2 mg/L
Nominal / measured:
nominal
Conc. based on:
other: carbon
Basis for effect:
mobility
Results with reference substance (positive control):
Immobilisation of Daphnia to the reference substance was assessed at two concentrations, 1 and 2 mg/L. Percentage immobilisation was 40 and 100% respectively. The result were within the normal range and thereof the test can be considered valid
Validity criteria fulfilled:
yes
Remarks:
Validity criteria relating to reference substance are detailed and fullfilled
Conclusions:
The toxicity of the test substance was measured to Daphnia toxicity based on immbolisation. An EC50 of >2.0 mg/L was determined.
Executive summary:

The toxicity of test substance was assessed in a study conducted according to EC Directive 92/69/EEC and to GLP. Limited information is included in the study report. Daphnia magna were exposed to the test substance for 48 hours at a measured concentration of 2.0mg/L measured based on carbon content. Immobilisation was assessed over this time period. Controls and a reference stubstance were also included.

The 48 hour EC50 was determined at >2.0 mg/L based on immobilisation

Description of key information

Two studies are presented here for short term toxicity to invertebrates, both using read-across substances. Both studies are reliable (Klimisch 2). The key study was carried out on the read across substance Butylene Glycol Dicaprylate/Dicaprate and resulted in a 48 hour EC50 for Daphnia magna of >2.0 mg/L based on immobilisation. This study has been selected as the key study as it provides the most conservative assessment of toxicity.

 

The other study was carried out on the read across substance Decanoic acid, mixed diesters with octanoic acid and propylene glycol and resulted in a 48 hour EC50 for Daphnia magna of >2.7mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
2 mg/L

Additional information