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EC number: 236-501-8 | CAS number: 13410-01-0
Comparison of Formazan Production:
For the test item and the positive control, the following percentage values of mean formazan production were calculated in comparison to the mean of the negative controls:
Two tissues of the human skin model EpiDerm™ were treated with Sodium selenite for three minutes and one hour, respectively. In average, 25 mg of the solid test item were applied to each tissue and spread to match the tissue size. One valid experiment was performed.
Deionised water was used as negative control, 8m KOH was used as positive control. After treatment, the respective substance was rinsed from the tissue; then, cell viability of the tissues was evaluated by addition of MTT which can be reduced to a blue formazan. Formazan production was measured by measuring the optical density (OD) of the resulting solution. After treatment with the negative control, the absorbance values were well above the required acceptability criterion of mean OD > 0.8 for both treatment intervals thus showing the quality of the tissues. The positive control showed clear corrosive effects for both treatment intervals.
After three minutes treatment with the test item, the relative absorbance values were reduced to 83.9 %. This value is well above the threshold for corrosion potential (50 %). After one hour treatment, relative absorbance values were reduced to 55.4 %. This value, too, is well above the threshold for corrosion potential (15 %).
In the guideline, values greater or equal to the threshold are considered as "non-corrosive to skin".
Therefore, Sodium selenite is considered as not corrosive in the Human Skin Model Test.
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