[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

2019

Justification for type of information:

Please refer to read-across justification attached in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

1986

Justification for type of information:

Please refer to read-across justification attached in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 - < 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

<= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Description of key information

In a 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the source test item had no toxic effects on the mobility of Daphnia up to the concentration of 100 mg/L. The 48-h EC50 was determined to be higher than 100 mg/L and the 48-h NOEC was determined to be 100 mg/L. A 24-h immobilisation study with Daphnia magna supports the obtained results in the 48-h test. The 24-h EC50 was determined to be >100 mg/L.

Additional information

No data on the substance itself is available. Therefore, read-across was applied to the source substance Phosphoric acid, 2-ethylhexyl ester, sodium salt (CAS 68186-64-1). This approach is considered appropriate since both substances are UVCB substances consisting of a mono-, bis- and tris- substituted phosphate with C-chains (4 - 6 C), only the cation is different. In the target substance the cation is ammonium, while the source substance has sodium.

Key:

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202, Regulation (EC) No. 440/2008 Method C.2 and EPA OPPTS 850.1010. The study was conducted in a static system over a period of 48 hours with a limit concentration of 100 mg/L. 20 daphnids per test concentration and control were tested. The test item concentration was determined at the start and at the end of the test with LC/MS/MS and it was in the range of 91 - 97 % of the nominal concentration. Therefore, the biological endpoints were based on the nominal concentration. The validity criteria of the test guideline were fulfilled. There was no immobilisation observed in the twenty daphnids exposed to the test item treated and control groups. Further no abnormal behaviour or appearance of test animals was noted. The estimated EC50 value (48 hours) of the test item based on the nominal concentration is >100 mg/L. The NOEC was determined to be 100 mg/L.

Supporting:

The 24-h acute toxicity of the test item to Daphnia magna was studied under static conditions according to OECD 202 (1983) and EU method C.2 (1984). Daphnids were exposed to the test item at nominal concentrations of 10, 100 and 1000 mg/L. No analytical monitoring was performed. During the study the dissolved oxygen, pH, water temperature and the immobilization rate were measured. Based on the number of immobile daphnids the EC values were determined. The EC0 value was > 100 mg/L (nominal) and the EC100 was ≤ 1000 mg/L after 24 hours. The EC50 value was determined to be > 100 mg/L.

Based on the structural similarities between the source and the target substance, the target substance is also considered to not be toxic to aquatic invertebrates up to the limit concentration.