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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol
EC Number:
414-810-0
EC Name:
A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol
Cas Number:
144163-85-9
Molecular formula:
Empirical formula: C25H35N2O5
IUPAC Name:
butanedioic acid; propan-2-ol; bis(tert-butyl N-[(4R)-5-amino-4-hydroxy-1,6-diphenylhexan-2-yl]carbamate)

Sampling and analysis

Details on sampling:
As each culture reached 3 hours contact time its pH was recroded and the rate of respiration measured using a Yellow springs Oxygen Meter (Model No. 57) and a magnetic stirrer. a 280 ml darkened glass BOD bottle was completely filled with the culture and seald by the dissolved oxygen probe. The dissolved oxygen level was recorded for approximately 10 minutes (between approximately 6.5 and 2.5 mgO2/l). separate mesuring bottles were used for control test and reference cultures to eliminate the possibility of cross contamination.

Test solutions

Details on test solutions:
reference substance = 3,5-dichlorophenol (C6H3Cl2.OH) @ 97% purity. Conc = 3.2, 10 and 32 mg/l

test substance: 10, 22, 46, 100 and 220 mg/l (nominal concentrations)

Test organisms

Test organisms (species):
sewage, domestic
Details on inoculum:
Temp = 20 +/- oC
pH = 7.2
suspended solids = 5.7 mg/l
dilution rate 1:1
final suspended solids level = 2.85 g/l

Study design

Test type:
other:
Total exposure duration:
3 h
Post exposure observation period:
after 3 hours contact time for each culture, the rate of respiration and pH was measured

Test conditions

Hardness:
120-180 mg/l CaCO3
Test temperature:
21 oC
pH:
pH of water = 7.5 (+/ 0.5)
Nominal and measured concentrations:
nominal conc of test substance = 3.2, 10, 32 mg/l
Details on test conditions:
temp = 21 oC under normal laboratory lighting

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
59 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Details on results:
The EC 50 (respiration ihibition) value for A-88820.605 with activated sewage sludge has been determinded as 59 mg/l (nominal concentration) for the 3 hour contact time.

Applicant's summary and conclusion

Conclusions:
The EC 50 (respiration ihibition) value for A-88820.605 with activated sewage sludge has been determinded as 59 mg/l (nominal concentration) for the 3 hour contact time.