Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under GLP and OECD method

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
Method C1, Directive 92/69/EEC
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
430-910-7
EC Name:
-
Cas Number:
13335-71-2
Molecular formula:
Hill formula: C10H12O3 CAS formula: C10H12O3
IUPAC Name:
2-(2,6-dimethylphenoxy)acetic acid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples withdrawn at 0 and 24h

Test solutions

Vehicle:
no
Details on test solutions:
No auxiliary solvents were required.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Zebra fish: Brachydanio rerio
weight = 0.36g
length = 3cm

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
96 hours

Test conditions

Hardness:
247 mg of CaCO3
Test temperature:
23 oC
pH:
7.8
Dissolved oxygen:
8.4 mg/l
Nominal and measured concentrations:
Nominal conc= 10, 17, 28, 48, 80 mg/l
Measured conc = 9.56, 16.76, 28.05, 48.64, 81.61 mg/l
Details on test conditions:
exposed to artificial light 16 hours daily

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 45.91 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL lower = 37.72; 95% Cl upper = 55.87
Details on results:
First abnormalities observed at 48 mg/l at 72 hours
Reported statistics and error estimates:
LC 50: 95% CL lower = 37.72 mg/l ; 95% Cl upper = 55.87 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC50 for the test substance is estimated to be 45.91 mg/l at 96 hours