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EC number: 210-762-8 | CAS number: 622-97-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: Federal Hazardous Substances Act , September 27, 1973
- GLP compliance:
- no
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- New Zealand White rabbits weighing 2.05 to 2.15 kg.
- Type of coverage:
- open
- Preparation of test site:
- other: abraded and non-abraded site on each rabbit
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- Single dermal application of 0.5 mL to clipped and abraded rabbit skin. No occlusion. Not stated if wash off performed.
- Duration of treatment / exposure:
- Single application. 24 h exposure.
- Observation period:
- Observed at 24 and 72 h. Primary Index calculated.
- Number of animals:
- 3 male and 3 female
- Details on study design:
- The site of application was clipped and abraded on all rabbits. Observations were made at 24 and 72 h following application for signs of irritation and corrosivity. Irritation scores were recorded and the primary index was calculated. The test substance was applied to the backs and occlusive wrap was not used because the test substance reacted with the dental dam creating unwanted variable. The rabbits were restrained by being put in a Newman harness for the 24 h initial exposure.
- Irritation parameter:
- primary dermal irritation index (PDII)
- Basis:
- mean
- Time point:
- other: 24 and 72 h
- Score:
- 1.5
- Reversibility:
- other: reversibility not investigated
- Irritation parameter:
- erythema score
- Basis:
- animal: 1-6
- Time point:
- 24 h
- Score:
- 1.5
- Max. score:
- 2
- Reversibility:
- not fully reversible within: 72 h
- Remarks on result:
- other: for both intact and abraded skin
- Irritation parameter:
- edema score
- Basis:
- animal: 1-6
- Time point:
- 24 h
- Score:
- 0.3
- Max. score:
- 1
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- other: for both intacts and abraded skin
- Irritation parameter:
- erythema score
- Basis:
- animal: 1-6
- Time point:
- 48 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Basis:
- animal: 1-6
- Time point:
- 48 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- erythema score
- Basis:
- animal: 1-6
- Time point:
- 72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- not fully reversible within: 72 h
- Remarks on result:
- other: for noth intact and abraded skin
- Irritation parameter:
- edema score
- Basis:
- animal: 1-6
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 1
- Reversibility:
- fully reversible
- Remarks on result:
- other: for both intact and abraded skin
- Irritant / corrosive response data:
- Well defined erythema (score 2) in 3/6 rabbits with very slight edema (score 1) in other 3/6 rabbits was apparent at 24 h. At 72 h only very slight erythema (score 1) and edema (score 1) remained.
Mean value of > 1.5 - < 2.3 for erythema/eschar or for oedema from gradings in at least 2 of 3 tested animals from grades at 24, 48 and 72 h or, if reactions are delayed, from grades on 3 consecutive days after the onset of dermal reactions (when not included in the irritant category above).
In this study reactions were only measured at 24 and 72 h. However, at 24 hurs 3/6 animals had a score of 2 for erythema and 3/6 a score of 1. At 24 h 6/6 had a score of 1. Therefore the mean score for 3/6 rabbits is 1.5 - Conclusions:
- Under the study conditions, the calculated primary index for the test substance was 1.5.
- Executive summary:
A study was conducted to determine the skin irritation/corrosion potential of the test substance in rabbits following a single dermal exposure according to Federal Hazardous Substances Act, September 27, 1973. Six New Zealand White rabbits (2.05 to 2.15 kg) were dosed dermally with a single application of 0.5 mL of the test substance. The site of application was clipped and abraded on all rabbits. Observations were made at 24 and 72 h following application for signs of irritation and corrosivity. Irritation scores were recorded and the primary index was calculated. The test substance was applied to the backs and an occlusive wrap was not used. Reactions were only measured at 24 and 72 h. At 24 h, 3/6 animals had a score of 2 for erythema and 3/6 a score of 1. At 24 h, 6/6 had a score of 1. Under the study conditions, the calculated primary index for the test substance was 1.5 (Adamik, 1997).
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- summary only, lacking detail
- GLP compliance:
- no
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- New Zealand White rabbits weighing 2.00 to 2.25 kg.
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 0.1 mL instilled in to right eye. Left eye served as untreated control. Not stated if wash out performed.
- Duration of treatment / exposure:
- Once only. Not stated if washout performed.
- Observation period (in vivo):
- Observed at 1, 24, 48 and 72 hours and 4 and 7 days.
- Number of animals or in vitro replicates:
- 3 male and 3 female
- Details on study design:
- Signs of ulceration of the cornea, corneal opacity, conjunctivitis, iris irritation, chemosis, tearing and refuse color were recorded.
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: All
- Time point:
- 24/48/72 h
- Remarks on result:
- other: information not available
- Irritation parameter:
- iris score
- Basis:
- animal: All
- Time point:
- 24/48/72 h
- Remarks on result:
- other: information not available
- Irritation parameter:
- conjunctivae score
- Remarks:
- Redness
- Basis:
- animal: All
- Time point:
- 24/48/72 h
- Remarks on result:
- other: information not available
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 7 d
- Score:
- 0
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 7 d
- Score:
- 0
- Irritation parameter:
- chemosis score
- Basis:
- animal: All
- Time point:
- 24/48/72 h
- Remarks on result:
- other: information not available
- Irritant / corrosive response data:
- Immediate responses following ocular instillation – All of the rabbits closed the treated eye and preened themselves when returned to their cage. Within five minutes after instillation the rabbits had the right treated eye open. One rabbit vocalized for a few seconds when put back into its cage.
- Conclusions:
- Under the study conditions, there were signs of immediate discomfort (closing of eye, vocalisation) and slight chemosis, erythema and discharge, However, the maximum scores given were 1 and all signs were reversible within 7 d.
- Executive summary:
A study was conducted to determine the eye irritation potential of the test substance in rabbit following a single ocular exposure according to Federal Hazardous Substances Act, September 27, 1973. Three male and 3 female New Zealand White rabbits were exposed to 0.1 mL of the test substance instilled into the right eye. It was not stated whether a wash out was performed. Under the study conditions, there were signs of immediate discomfort (closing of eye, vocalisation) and slight chemosis, erythema and discharge, However, the maximum scores given were 1 and all signs were reversible within 7 d (Adamik, 1977).
Reference
Mean Eye irritation scores for each rabbit:
Rabbit N |
Mean of Score at 24, 48 and 72 Hours |
||||
Corneal Opacity |
Iris Lesion |
Conjunctivae |
|||
Chemosis |
Erythema |
Discharge |
|||
1 |
0 |
0 |
0 |
0 |
0.33 |
2 |
0 |
0 |
0 |
1 |
0.66 |
3 |
0 |
0 |
0 |
0.33 |
0 |
4 |
0 |
0 |
0 |
0 |
0 |
5 |
0 |
0 |
0 |
1 |
0 |
6 |
0 |
0 |
0 |
0.33 |
0.33 |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin
A study was conducted to determine the skin irritation/corrosion potential of the test substance in rabbits following a single dermal exposure according to Federal Hazardous Substances Act, September 27, 1973. Six New Zealand White rabbits (2.05 to 2.15 kg) were dosed dermally with a single application of 0.5 mL of the test substance. The site of application was clipped and abraded on all rabbits. Observations were made at 24 and 72 h following application for signs of irritation and corrosivity. Irritation scores were recorded and the primary index was calculated. The test substance was applied to the backs and an occlusive wrap was not used. Reactions were only measured at 24 and 72 h. At 24 h, 3/6 animals had a score of 2 for erythema and 3/6 a score of 1. At 24 h, 6/6 had a score of 1. Under the study conditions, the calculated primary index for the test substance was 1.5 (Adamik, 1997).
Eye
A study was conducted to determine the eye irritation potential of the test substance in rabbit following a single ocular exposure according to Federal Hazardous Substances Act, September 27, 1973. Three male and 3 female New Zealand White rabbits were exposed to 0.1 mL of the test substance instilled into the right eye. It was not stated whether a wash out was performed. Under the study conditions, there were signs of immediate discomfort (closing of eye, vocalisation) and slight chemosis, erythema and discharge, However, the maximum scores given were 1 and all signs were reversible within 7 d (Adamik, 1977).
Justification for classification or non-classification
Based on in vivo irritation testing in rabbit, the test substance does not appear to be irritating to skin or eyes to a degree triggering classification according to CLP (EC 1272/2008) criteria. However, severe irritation was noted in a 21 d repeated dose toxicity test in rabbit. Also, the read across substance vinyl toluene has been reported to be irritating to the eyes, upper respiratory tract and skin of humans at concentrations greater than 400 ppm. Based on this additional evidence, classification as Skin Irrit. 2 – H315 (causes skin irritation) and Eye Irrit. 2 – H319 (causes serious eye irritation) is therefore proposed.
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