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Acute Toxicity: oral

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Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
yes
Specific details on test material used for the study:
no details given
Species:
rat
Strain:
Sprague-Dawley
Sex:
not specified
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250g and 290g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.
Type of coverage:
occlusive
Vehicle:
other: 0.8% aqueous hydroxypropyl-methyl-cellulose gel
Details on dermal exposure:
The hair on the site of application was clipped with hair-clippers, without causing injury, approximately 24 hours before application.
The site was situated on the animal's back between the fore and hind extremitites and had an area of approx. 5 x 6 cm (=1/10 body surface).
The test substance was applied to 8 layers of gauze and then to the application site. The patch was covered with a plastic sheet and secured with adhesive plaster.
Duration of exposure:
24 hours
Doses:
2500 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration; then twice daily for a period of 14 days
- Frequency of body weights: before administration, then weekly for a period of 14 days
- Necropsy of survivors performed: yes
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 2 500 mg/kg bw
Based on:
test mat.
Sex:
not specified
Dose descriptor:
other: no-effect level
Effect level:
> 2 500 mg/kg bw
Based on:
test mat.
Remarks on result:
other: no signs of toxicity were observed
Mortality:
Mortality did not occur in treated animals.
Clinical signs:
No signs of toxicity were observed following dermal application.
Body weight:
No signs of toxicity were observed following dermal application.
Other findings:
No signs of toxicity were observed following dermal application.
Conclusions:
No mortality occurred at a tested level of 2500 mg/kg bw. The LD50 is considered to be more than 2500 mg/kg bw.
Executive summary:

In an acute dermal toxicity study (limit test similar to OECD Guideline 402), groups of Sprague-Dawley rats (5/group) were given a single dermal dose of vanadium pentoxide, at 2500 mg/kg bw and observed for 14 days. No mortality occurred in this test; therefore an exact LD50 has not been determined. The test article vanadium pentoxide, when administered dermally as received to 5 Sprague Dawley rats had an acute dermal LD50 of greater than 2.5 g/kg bw. No evidence of systemic toxicity was observed.

Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
no guideline followed
Principles of method if other than guideline:
not specified
GLP compliance:
not specified
Limit test:
no
Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250g and 290g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.
Route of administration:
inhalation: dust
Type of inhalation exposure:
nose only
Vehicle:
air
Mass median aerodynamic diameter (MMAD):
> 7.22 - < 13.26 µm
Remark on MMAD/GSD:
An analysis of the particle size distribution was carried out twice during the exposure period using a cascade impactor according to May (May K. R. (1975) J. Aerosol. Sei. 6: 413).
The dust from the exposure chamber was sucked through the cascade impactor for 1.5 to 5 min at a constant flow rate of 5 l/min. The slides covered with adhesive tape were removed from the impactor and were weighted on an analytical balance (precision 10 µg). The mass median aerodynamic diameter (MMAD) was estimated by means of nonlinear regression analysis (Litchfield and Wilcoxon, (1949) J. Pharmacol. 95: 99).
Details on inhalation exposure:
Analysis of the Dust Concentration:
The dust concentration in the inhalation chamber was measured with an air sample filter (Sartorius Minisart SM 17598). Dust samples were taken during the first half and during the second half of the exposure. For that purpose, Minisart SM 17598 filters were placed close to the animals' nose and sucked through with a constant flow of air of 300 l/h for 1 to 3 min. The filters were weighed before and after sampling. Concentration of the test substance in the air was calculated as mg/l.
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Control animals:
not specified
Details on study design:
The study was carried out using a dynamic inhalation apparatus with a nose only exposure of the animals. The apparatus consists of a cylindrical exposure chamber (volume 40 l) which holds a maximum of 20 animals in pyrex tubes at the edge of the chamber in a radial position.
The dust was generated with a dust generator and dosing apparatus. The generator was fed with compressed air from a compressor. At the bottom of the exposure chamber the air was sucked off at a similar rate as created by the dust generator, in order to produce a homogeneous distribution in the exposure chamber. At least 12 air changes per hour were carried out. Air-flow meters were used to control the constant supply of compressed air and vacuum. Flow rates were checked at least once hour and corrected if necessary. Exposure time was 4 hours.
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971
Key result
Sex:
male
Dose descriptor:
LC50
Effect level:
2.61 mg/L air
Based on:
not specified
Key result
Sex:
female
Dose descriptor:
LC50
Effect level:
2.43 mg/L air
Based on:
not specified
Sex:
male
Dose descriptor:
other: No-effect level
Effect level:
0.72 mg/L air
Based on:
not specified
Sex:
female
Dose descriptor:
other: No-effect level
Effect level:
1.21 mg/L air
Based on:
not specified
Mortality:
Animals died up to 8 days after the inhalative exposure.
Other findings:
Histopathological examination of the lungs of the animals that received inhalative exposure revealed haemorrhage, vascular congestion and oedema in the lungs and bronchopneumonia.
Conclusions:
LC50 = 2.61 mg/L (male rats), 2.43 mg/L (female rats)
Executive summary:

Rats were exposed to dust of ammonium vanadate (NH4VO3, CAS 7803 -55 -6) for 4 hours (nose only). Animals died up to 8 days after the inhalative exposure. Histopathological examination of the lungs of the animals that received inhalative exposure revealed haemorrhage, vascular congestion and oedema in the lungs and bronchopneumonia. The LC50-values for V2O3(analytical grade, powder) is 6.65 mg/L in female rats. The LC50-values for the NH4VO3 test-substance following inhalative exposure were 2.61 mg/L for male and 2.43 mg/L for female rats.

Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
no guideline followed
Principles of method if other than guideline:
not specified
GLP compliance:
not specified
Limit test:
no
Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250g and 290g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.
Route of administration:
inhalation: dust
Type of inhalation exposure:
nose only
Vehicle:
air
Mass median aerodynamic diameter (MMAD):
> 15.14 - < 19.48 µm
Remark on MMAD/GSD:
An analysis of the particle size distribution was carried out twice during the exposure period using a cascade impactor according to May (May K. R. (1975) J. Aerosol. Sei. 6: 413).
The dust from the exposure chamber was sucked through the cascade impactor for 1.5 to 5 min at a constant flow rate of 5 l/min. The slides covered with adhesive tape were removed from the impactor and were weighted on an analytical balance (precision 10 µg). The mass median aerodynamic diameter (MMAD) was estimated by means of nonlinear regression analysis (Litchfield and Wilcoxon, (1949) J. Pharmacol. 95: 99).
Details on inhalation exposure:
Analysis of the Dust Concentration:
The dust concentration in the inhalation chamber was measured with an air sample filter (Sartorius Minisart SM 17598). Dust samples were taken during the first half and during the second half of the exposure. For that purpose, Minisart SM 17598 filters were placed close to the animals' nose and sucked through with a constant flow of air of 300 l/h for 1 to 3 min. The filters were weighed before and after sampling. Concentration of the test substance in the air was calculated as mg/l.
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Control animals:
not specified
Details on study design:
The study was carried out using a dynamic inhalation apparatus with a nose only exposure of the animals. The apparatus consists of a cylindrical exposure chamber (volume 40 l) which holds a maximum of 20 animals in pyrex tubes at the edge of the chamber in a radial position.
The dust was generated with a dust generator and dosing apparatus. The generator was fed with compressed air from a compressor. At the bottom of the exposure chamber the air was sucked off at a similar rate as created by the dust generator, in order to produce a homogeneous distribution in the exposure chamber. At least 12 air changes per hour were carried out. Air-flow meters were used to control the constant supply of compressed air and vacuum. Flow rates were checked at least once hour and corrected if necessary. Exposure time was 4 hours.
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971
Sex:
male
Dose descriptor:
LC50
Remarks on result:
not determinable
Key result
Sex:
female
Dose descriptor:
LC50
Effect level:
> 6.65 mg/L air
Based on:
not specified
Sex:
male
Dose descriptor:
other: No-effect level
Remarks on result:
not determinable
Sex:
female
Dose descriptor:
other: No-effect level
Effect level:
> 6.65 mg/L air
Based on:
not specified
Mortality:
Animals died up to 8 days after the inhalative exposure.
Other findings:
Histopathological examination of the lungs of the animals that received inhalative exposure revealed haemorrhage, vascular congestion and oedema in the lungs and bronchopneumonia.
Conclusions:
LC50 > 6.65 mg/l (female rats)
Executive summary:

Rats were exposed to dust of vanadium (III) oxide (V2O3,CAS 1314 -34 -7) for 4 hours (nose only). Animals died up to 8 days after the inhalative exposure. Histopathological examination of the lungs of the animals that received inhalative exposure revealed haemorrhage, vascular congestion and oedema in the lungs and bronchopneumonia. The LC50-values for V2O3 (analytical grade, powder) is > 6.65 mg/L in female rats.

Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
not specified
Principles of method if other than guideline:
Oral Toxicity:
The test substance was suspended in 0.8 % aqueous hydroxypropyl-methyl-cellulose gel and administered by gavage. The volume of application was 20 ml/kg b.w.
Clinical Evaluation:
Following administration, observations were made and recorded systematically with individual records being maintained for each animal. Observations were performed immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration. Then twice daily for a period of 14 days. The time of death was recorded as precisely as possible. Individual body weights were recorded before administration of the substance, thereafter in weekly intervals up to the end of the study, and at death. At the end of the experiments all surviving animals were sacrificed, dissected and inspected macroscopically. All gross pathological changes were recorded. Autopsy and macroscopic inspection of rats which died prematurely were carried out as soon as possible after exitus.
GLP compliance:
not specified
Limit test:
no
Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250g and 290g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.
Route of administration:
oral: gavage
Vehicle:
methylcellulose
Details on oral exposure:
VEHICLE
- Amount of vehicle (if gavage): 20 ml/kg b.w.
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
218 mg/kg bw
Based on:
not specified
Key result
Sex:
female
Dose descriptor:
LD50
Effect level:
141 mg/kg bw
Based on:
not specified
Sex:
male
Dose descriptor:
other: No-effect level
Effect level:
100 mg/kg bw
Based on:
not specified
Sex:
female
Dose descriptor:
other: No-effect level
Effect level:
46.4 mg/kg bw
Based on:
not specified
Mortality:
Animals died up to 8 days after the oral exposure.
Clinical signs:
Following oral administration reduced motility, ataxia, muscular hypotonia, dyspnoea, reduced body weight gain and death were observed as toxic signs.
Gross pathology:
Macrosopic inspection revealed haemorrhagic intestinal mucosa and a dark liver following oral exposure in the animals that died prematurely.
Interpretation of results:
Category 3 based on GHS criteria
Conclusions:
LD50 = 218 mg/kg bw(male rats), 141 mg/kg bw (female rats)
Executive summary:

The acute oral toxicity of ammonium metavanadate (CAS 7803 -55 -6) was studies similar to OECD Guideline 401. Following administration, observations were made and recorded systematically with individual records being maintained for each animal. Observations were performed immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration. Then twice daily for a period of 14 days. The time of death was recorded as precisely as possible. Individual body weights were recorded before administration of the substance, thereafter in weekly intervals up to the end of the study, and at death. At the end of the experiments all surviving animals were sacrificed, dissected and inspected macroscopically. All gross pathological changes were recorded. Autopsy and macroscopic inspection of rats which died prematurely were carried out as soon as possible after exitus. The LD50-values for the NH4VO3 test-substance (analytical grade, powder) following oral administration were 218 mg/kg bw for male and 141 mg/kg bw for female rats.

Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
no guideline followed
Principles of method if other than guideline:
not specified
GLP compliance:
not specified
Limit test:
no
Specific details on test material used for the study:
no details given
Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250g and 290g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.
Route of administration:
inhalation: dust
Type of inhalation exposure:
nose only
Vehicle:
air
Mass median aerodynamic diameter (MMAD):
> 2.01 - < 2.44 µm
Remark on MMAD/GSD:
An analysis of the particle size distribution was carried out twice during the exposure period using a cascade impactor according to May (May K. R. (1975) J. Aerosol. Sei. 6: 413).
The dust from the exposure chamber was sucked through the cascade impactor for 1.5 to 5 min at a constant flow rate of 5 l/min. The slides covered with adhesive tape were removed from the impactor and were weighted on an analytical balance (precision 10 µg). The mass median aerodynamic diameter (MMAD) was estimated by means of nonlinear regression analysis (Litchfield and Wilcoxon, (1949) J. Pharmacol. 95: 99).
Details on inhalation exposure:
Analysis of the Dust Concentration:
The dust concentration in the inhalation chamber was measured with an air sample filter (Sartorius Minisart SM 17598). Dust samples were taken during the first half and during the second half of the exposure. For that purpose, Minisart SM 17598 filters were placed close to the animals' nose and sucked through with a constant flow of air of 300 l/h for 1 to 3 min. The filters were weighed before and after sampling. Concentration of the test substance in the air was calculated as mg/l.
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
The study was carried out using a dynamic inhalation apparatus with a nose only exposure of the animals. The apparatus consists of a cylindrical exposure chamber (volume 40 l) which holds a maximum of 20 animals in pyrex tubes at the edge of the chamber in a radial position.
The dust was generated with a dust generator and dosing apparatus. The generator was fed with compressed air from a compressor. At the bottom of the exposure chamber the air was sucked off at a similar rate as created by the dust generator, in order to produce a homogeneous distribution in the exposure chamber. At least 12 air changes per hour were carried out. Air-flow meters were used to control the constant supply of compressed air and vacuum. Flow rates were checked at least once hour and corrected if necessary. Exposure time was 4 hours.
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971
Key result
Sex:
male
Dose descriptor:
LC50
Effect level:
11.09 mg/L air
Based on:
not specified
Key result
Sex:
female
Dose descriptor:
LC50
Effect level:
4.3 mg/L air
Based on:
not specified
Sex:
male
Dose descriptor:
other: No-effect level
Effect level:
0.9 mg/L air
Based on:
not specified
Sex:
female
Dose descriptor:
other: No-effect level
Effect level:
2.42 mg/L air
Based on:
not specified
Mortality:
Animals died up to 8 days after the inhalative exposure.
Other findings:
Histopathological examination of the lungs of the animals that received inhalative exposure revealed haemorrhage, vascular congestion and oedema in the lungs and bronchopneumonia
Conclusions:
LC50 = 11.09 mg/l (male rats), 4.3 mg/l (female rats)
Executive summary:

Rats were exposed to dust of vanadium pentoxide (V2O5, CAS 1314 -62 -1) for 4 hours (nose only). Animals died up to 8 days after the inhalative exposure. Histopathological examination of the lungs of the animals that received inhalative exposure revealed haemorrhage, vascular congestion and oedema in the lungs and bronchopneumonia. The LC50-values for the V2O5 test-substance (analytical grade, powder) were 11.09 mg/L for male and 4.3 mg/L for female rats.

Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
not specified
Principles of method if other than guideline:
Oral Toxicity:
The test substance was suspended in 0.8 % aqueous hydroxypropyl-methyl-cellulose gel and administered by gavage. The volume of application was 20 ml/kg b.w.
Clinical Evaluation:
Following administration, observations were made and recorded systematically with individual records being maintained for each animal. Observations were performed immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration. Then twice daily for a period of 14 days. The time of death was recorded as precisely as possible. Individual body weights were recorded before administration of the substance, thereafter in weekly intervals up to the end of the study, and at death. At the end of the experiments all surviving animals were sacrificed, dissected and inspected macroscopically. All gross pathological changes were recorded. Autopsy and macroscopic inspection of rats which died prematurely were carried out as soon as possible after exitus.
GLP compliance:
not specified
Limit test:
no
Specific details on test material used for the study:
no details given
Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250g and 290g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.
Route of administration:
oral: gavage
Vehicle:
methylcellulose
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 20 ml/kg bw
Doses:
not specified
No. of animals per sex per dose:
not specified
Control animals:
not specified
Details on study design:
no details given
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
8 713 mg/kg bw
Based on:
test mat.
Key result
Sex:
female
Dose descriptor:
LD50
Effect level:
5 639 mg/kg bw
Based on:
test mat.
Sex:
male
Dose descriptor:
other: No-effect level
Effect level:
4 640 mg/kg bw
Based on:
test mat.
Sex:
female
Dose descriptor:
other: No-effect level
Effect level:
4 640 mg/kg bw
Based on:
test mat.
Mortality:
Animals died up to 8 days after the oral exposure.
Clinical signs:
Following oral administration reduced motility, ataxia, muscular hypotonia, dyspnoea, reduced body weight gain and death were observed as toxic signs.
Gross pathology:
Macrosopic inspection revealed haemorrhagic intestinal mucosa and a dark liver following oral exposure in the animals that died prematurely.
Conclusions:
LD50 = 8713 mg/kg (male rats), 5639 mg/kg (female rats)
Executive summary:

The acute oral toxicity of vanadium (III) oxide (CAS 1314 -34 -7) was studies similar to OECD Guideline 401. Following administration, observations were made and recorded systematically with individual records being maintained for each animal. Observations were performed immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration. Then twice daily for a period of 14 days. The time of death was recorded as precisely as possible. Individual body weights were recorded before administration of the substance, thereafter in weekly intervals up to the end of the study, and at death. At the end of the experiments all surviving animals were sacrificed, dissected and inspected macroscopically. All gross pathological changes were recorded. Autopsy and macroscopic inspection of rats which died prematurely were carried out as soon as possible after exitus.

The LD50-values for the V2O3 test-substance (analytical grade, powder) following oral administration were 8713 mg/kg for male and 5639 mg/kg for female rats.

Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
yes
Species:
rat
Strain:
Sprague-Dawley
Sex:
not specified
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250 g and 290 g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.
Type of coverage:
occlusive
Vehicle:
other: 0.8% aqueous hydroxypropyl-methyl-cellulose gel
Details on dermal exposure:
The hair on the site of application was clipped with hair-clippers, without causing injury, approximately 24 hours before application.
The site was situated on the animal's back between the fore and hind extremitites and had an area of approx. 5 x 6 cm (=1/10 body surface).
The test substance was applied to 8 layers of gauze and then to the application site. The patch was covered with a plastic sheet and secured with adhesive plaster.
Duration of exposure:
24 hours
Doses:
2500 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration; then twice daily for a period of 14 days
- Frequency of body weights: before administration, then weekly for a period of 14 days
- Necropsy of survivors performed: yes
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971
Preliminary study:
not performed
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 2 500 mg/kg bw
Based on:
test mat.
Sex:
not specified
Dose descriptor:
other: no-effect level
Effect level:
> 2 500 mg/kg bw
Based on:
test mat.
Remarks on result:
other: no signs of toxicity were observed
Mortality:
Mortality did not occur in treated animals.
Clinical signs:
No signs of toxicity were observed following dermal application.
Body weight:
No signs of toxicity were observed following dermal application.
Gross pathology:
not performed
Other findings:
No signs of toxicity were observed following dermal application.
Interpretation of results:
GHS criteria not met
Conclusions:
No mortality occurred at a tested level of 2500 mg/kg bw. The LD50 is considered to be more than 2500 mg/kg bw.
Executive summary:

In an acute dermal toxicity study (limit test similar to OECD Guideline 402), groups of Sprague-Dawley rats (5/group) were given a single dermal dose of ammonium metavanadate, at 2500 mg/kg bw and observed for 14 days. No mortality occurred in this test; therefore an exact LD50 has not been determined. The test article, when administered dermally as received to 5 Sprague Dawley rats had an acute dermal LD50 of greater than 2.5 g/kg bw. No evidence of systemic toxicity was observed.

Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer-reviewed data
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
yes
Specific details on test material used for the study:
no details given
Species:
rat
Strain:
Sprague-Dawley
Sex:
not specified
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250 g and 290 g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.

Type of coverage:
occlusive
Vehicle:
other: 0.8% aqueous hydroxypropyl-methyl-cellulose gel
Details on dermal exposure:
The hair on the site of application was clipped with hair-clippers, without causing injury, approximately 24 hours before application.
The site was situated on the animal's back between the fore and hind extremities and had an area of approx. 5 x 6 cm (=1/10 body surface).
The test substance was applied to 8 layers of gauze and then to the application site. The patch was covered with a plastic sheet and secured with adhesive plaster.
Duration of exposure:
24 hours
Doses:
2500 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration; then twice daily for a period of 14 days
- Frequency of body weights: before administration, then weekly for a period of 14 days
- Necropsy of survivors performed: yes
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971
Preliminary study:
not performed
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 2 500 mg/kg bw
Based on:
test mat.
Sex:
not specified
Dose descriptor:
other: no-effect level
Effect level:
> 2 500 mg/kg bw
Based on:
test mat.
Remarks on result:
other: no signs of toxicity were observed
Mortality:
Mortality did not occur in treated animals.
Clinical signs:
No signs of toxicity were observed following dermal application.
Body weight:
No signs of toxicity were observed following dermal application.
Gross pathology:
not performed
Other findings:
No signs of toxicity were observed following dermal application.
Interpretation of results:
GHS criteria not met
Conclusions:
No mortality occurred at a tested level of 2500 mg/kg bw. The LD50 is considered to be > 2500 mg/kg bw.
Executive summary:

In an acute dermal toxicity study (limit test similar to OECD Guideline 402), groups of Sprague-Dawley rats (5/group) were given a single dermal dose of vanadium pentoxide, at 2500 mg/kg bw and observed for 14 days. No mortality occurred in this test; therefore an exact LD50 has not been determined. The test article vanadium pentoxide, when administered dermally as received to 5 Sprague Dawley rats had an acute dermal LD50 of greater than 2.5 g/kg bw. No evidence of systemic toxicity was observed.

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
Vanadium and its inorganic compounds (inhalable fraction)
Year:
2009
Bibliographic source:
The MAK-Collection Part I: MAK Value Documentations, Vol. 25. DFG, Deutsche Forschungsgemeinschaft
Reference Type:
publication
Title:
New Investigations on Acute Toxicities of Vanadium Oxides
Author:
J. Leuschner et al.
Year:
1994
Bibliographic source:
Monatshefte für Chemie 125. 623-646

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
not specified
Principles of method if other than guideline:
Oral Toxicity:
The test substance was suspended in 0.8 % aqueous hydroxypropyl-methyl-cellulose gel and administered by gavage. The volume of application was 20 ml/kg b.w.
Clinical Evaluation:
Following administration, observations were made and recorded systematically with individual records being maintained for each animal. Observations were performed immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration. Then twice daily for a period of 14 days. The time of death was recorded as precisely as possible. Individual body weights were recorded before administration of the substance, thereafter in weekly intervals up to the end of the study, and at death. At the end of the experiments all surviving animals were sacrificed, dissected and inspected macroscopically. All gross pathological changes were recorded. Autopsy and macroscopic inspection of rats which died prematurely were carried out as soon as possible after exitus.
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Divanadium pentaoxide
EC Number:
215-239-8
EC Name:
Divanadium pentaoxide
Cas Number:
1314-62-1
Molecular formula:
O5V2
IUPAC Name:
dioxovanadiooxy(dioxo)vanadium
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
no details given

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
The animals used were Sprague Dawley rats (Lippische Versuchstierzucht Hagemann GmbH. Extertal, Germany) weighing between 250g and 290g. Rats were provided with standard rat diet and water ad libitum. The diet was withdrawn approx. 16 hours before application. At the onset of the main experiment animals were randomly divided into group of 5.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
methylcellulose
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 20 ml/kg bw
Doses:
not specified
No. of animals per sex per dose:
not specified
Control animals:
not specified
Details on study design:
no details given
Statistics:
The LD50 was calculated according to Finney D.J., Probit Analysis, 3rd Edition, Cambridge University Press, Cambridge, 1971

Results and discussion

Effect levelsopen allclose all
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
470 mg/kg bw
Based on:
not specified
Key result
Sex:
female
Dose descriptor:
LD50
Effect level:
467 mg/kg bw
Based on:
not specified
Sex:
male
Dose descriptor:
other: No-effect level
Effect level:
215 mg/kg bw
Based on:
not specified
Sex:
female
Dose descriptor:
other: No-effect level
Effect level:
215 mg/kg bw
Based on:
not specified
Mortality:
Animals died up to 8 days after the oral exposure.
Clinical signs:
Following oral administration reduced motility, ataxia, muscular hypotonia, dyspnoea, reduced body weight gain and death were observed as toxic signs.
Gross pathology:
Macrosopic inspection revealed haemorrhagic intestinal mucosa and a dark liver following oral exposure in the animals that died prematurely.

Applicant's summary and conclusion

Conclusions:
LD50 = 470 mg/kg (male rats), 467 mg/kg (female rats)
Executive summary:

The acute oral toxicity of vanadium pentoxide (CAS 1314 -62 -1) was studies similar to OECD Guideline 401. Following administration, observations were made and recorded systematically with individual records being maintained for each animal. Observations were performed immediately, 5, 15, 30 and 60 min, as well as 3 h, 6 h and 24 h after administration. Then twice daily for a period of 14 days. The time of death was recorded as precisely as possible. Individual body weights were recorded before administration of the substance, thereafter in weekly intervals up to the end of the study, and at death. At the end of the experiments all surviving animals were sacrificed, dissected and inspected macroscopically. All gross pathological changes were recorded. Autopsy and macroscopic inspection of rats which died prematurely were carried out as soon as possible after exitus.The LD50-values for the V2O5 test-substance (analytical grade, powder) following oral administration were 470 mg/kg for male and 467 mg/kg for female rats.