Cyclohexyldimethylammonium dihydrogen phosphate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure following national standard guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard test guideline, DIN 38 412 L15, 1982. Comparable to OECD 203.

GLP compliance:

no

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Dimethylcyclohexylamine- Purity: 99.9 %- Lot/batch No.: II/87

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):Not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

No data provided

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Subsequently the fish were placed into the test aquaria

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM- Common name: Golden orfe- Strain: : Leuciscus idus L. (golden variety)- Source: Fish farm Paul Eggers, 2354 Hohenwestedt, Germany- Length at study initiation (length definition, mean, range and SD): 5.3 cm (range 4.7 - 5.7 cm)- Weight at study initiation (mean and range, SD): 3.7 g (range 2.1 - 4.9 g)- Feeding during test: no - Frequency: Withdrawal of food before exposure: 1 dayACCLIMATION- Acclimation period: 4 weeks - Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through systemWater temperature: 20 °C Duration of housing: about 4 weeksAcclimation to test water and test temperature: 3 days- Type and amount of food: : Growing feed F/B 50, - Feeding frequency: ad libitum.- Health during acclimation (any mortality observed):Mortality during the last 2 weeks of housing: 0.0 %Mortality during the acclimation period: 0 %Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

19 - 20 °C

pH:

about 8.0

Dissolved oxygen:

8.5 ± 0.5 mg/L

Salinity:

Not applicable

Nominal and measured concentrations:

nominal: 10.0, 21.5, 46.4, 100 mg/L. Additionally: 100 mg/L pH adjusted

Details on test conditions:

TEST SYSTEM- Test vessel: - Type (delete if not applicable): aquaria open - Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L - Aeration: aeration with oil-free air- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 1- Biomass loading rate: 3.7 g fish /LTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11- Ca/Mg ratio: 4 : 1- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hoursOTHER TEST CONDITIONS- Adjustment of pH: one test item concentration of 100 mg/L was pH adjusted- Photoperiod: 16:8 hour light:dark- Aeration: slight EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptomsTEST CONCENTRATIONS- Spacing factor for test concentrations: 2.2

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

31.58 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralised

Basis for effect:

mortality (fish)

Remarks on result:

other: geometrical mean

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Basis for effect:

mortality (fish)

Effect concentrations 5

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Neutralized, after pH adjustment

Basis for effect:

mortality (fish)

Remarks on result:

other: after pH-adjustment

Details on results:

There were no abnormalities.

Results with reference substance (positive control):

- Results with reference substance valid? Yes- LC50: 26 mg/L after 48 h

Reported statistics and error estimates:

Observation of the results.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Effects may be due to the pH of the substance: no effects were observed after 96 -h when exposed to a neutralized sample (100 mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Groups of ten Golden orfe (Leuciscus idus L., Golden variety) were exposed under static conditions to DMCHA, for a period of 96 -hours.  The 96 -hour LC50 was observed to be > 21.5 mg/L and < 46.4 mg/L, the corresponding no-observed effect concentration (NOEC) was 21.5 mg/L.  If the results are based on the pH adjusted medium the 96 hour LC would be >100 mg/L and the corresponding NOEC would be 100 mg/L.

Executive summary:

Groups of ten Golden orfe (Leuciscus idus L., Golden variety) were exposed under static conditions to cyclohexyldimethylamine (DMCHA), for a period of 96 -hours according to the German Standard Test Guideline, DIN 38 412 L15, 1982 which is comparable to OECD 203. Nominal exposure concentrations were control, 0.0, 21.5, 46.4, 100 mg/L, additionally: 100 mg/L pH adjusted. No analytical verification was performed therefore the results of the study were based on nominal exposure concentrations. After 96 hours 100% mortality was observed in the 46.4 and 100 (no pH adjustment) mg/L treatments, there was no mortality in any of the other treatments or control.

The toxic effect seen in the 46.4 and 100 mg/L (no pH adjustment) was due to the high pH levels in the test media. There was no mortality in the 100 mg/L test medium when the pH was adjusted to 7.8 prior to the addition of fish.

The 96 -hour LC50 was 31.6 mg/L (geometric mean), the corresponding no-observed effect concentration (NOEC) was 21.5 mg/L. 

If the results are based on the pH adjusted medium the 96 hour LC would be >100 mg/L and the corresponding NOEC would be 100 mg/L.