Menadione

Density

Administrative data

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental starting date: 05 July 2017. Experimental completion date: 23 August 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

air comparison pycnometer (for solids)

Test material

Test material information

Constituent 1

Reference substance name:

Menadione

EC Number:

200-372-6

EC Name:

Menadione

Cas Number:

58-27-5

Molecular formula:

C11H8O2

IUPAC Name:

2-methyl-1,4-dihydronaphthalene-1,4-dione

Specific details on test material used for the study:

Identification: Beta-Menadione
CAS No: 58-27-5
Description: light yellow powder
Batch number: J16090701
Purity: 99.2%
Expiry date: 06 September 2017
Storage conditions: room temperature, in the dark

Results and discussion

Density

Type:

relative density

Density:

1.38 other: D 20°C /4°C

Temp.:

20 °C

Any other information on results incl. tables

Calibration

The pressure readings and the calculated volume for the reference and sample cells are shown in the following table:

Determination	P1 (psig)	P2 (psig)	P3 (psig)	P4 (psig)	Reference Cell Volume (cm3)	Sample Cell Volume (cm3)
1	17.042	6.459	17.047	8.455	90.889	148.921
2	17.054	6.466	17.071	8.468	90.997	149.007
3	17.061	6.472	17.071	8.441	91.205	149.223

Mean reference cell volume : 91.030 cm3

Mean sample cell volume : 149.050 cm3

As the reference cell volume and sample cell volume were within the defined tolerance (90.953 ± 1.0 cm3 and 149.225 ± 1.0 cm3 for the reference and sample cell volumes respectively) the gas comparison pycnometer was therefore considered acceptable for use.

Sample

The pressure readings, calculated volumes and density values obtained for the test item are shown in the following table:

Determination	Replicate	P1(sample) (psig)	P2(sample) (psig)	Volume (cm3)	Density (kg/m3)	Mean Density (kg/m3)
1	A	17.067	6.879	14.2	1.38 x10^3	1.38 x10^3
	B	17.046	6.871	14.2	1.38 x10^3
	C	17.091	6.89	14.3	1.37 x10^3
2	A	17.032	6.769	11	1.39 x10^3	1.39 x10^3
	B	17.063	6.782	11.1	1.39 x10^3
	C	17.022	6.765	11	1.39 x10^3

Mean density : 1.38 x10^3 kg/m3

Relative density : 1.38

Temperature : 20.0 ± 0.5 °C

Duplicate determinations were performed to ensure a reproducibility of less than ± 20 kg/m3 was obtained.

Applicant's summary and conclusion

Conclusions:

The density of the test item has been determined to be 1.38 x103 kg/m3 at 20.0 ± 0.5 °C and a relative density of 1.38.

Executive summary:

Density. 1.38 x103 kg/m3 at 20.0 ± 0.5 °C, relative density of 1.38, using a gas comparison pycnometer, designed to be compatible with Method A.3 Relative Density of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 109 of the OECD Guidelines for Testing of Chemicals, 02 October 2012