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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
The test fish were received in November 2008 from import quarantine and were kept in internal quarantine for 14 days before the start of the study.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
No
Hardness:
250±25 mg/L calcium carbonate
Test temperature:
24±1°C
pH:
7.8±0.2
Dissolved oxygen:
>90% saturation
Salinity:
Not applicable
Conductivity:
Not applicable
Nominal and measured concentrations:
Nominal exposure concentrations: 0 (control), 400, 800, 1600, 3200 and 6400 mg/L.
Details on test conditions:
Fish were incubated under semi-static conditions with exchange of test and control solutions every 24 hours.

Dilution water was aerated ot oxygen saturation exceeding 90% with a pH of 7.8 +- 0.2. This fish were tested in 5L glass vessels. With normal laboratory illumination and a light dary cycle of 16:8. The following loading rates were used with 7 fish per rate: 0 (control), 400, 800, 1600, 3200, 6400 mg/L nominal.
Reference substance (positive control):
yes
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2 400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
800 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Details on results:
Mortality after 96 hours exposure was 0% in the control, 400, 800 and 1600 mg/L, 100% mortality was observed at 3200 and 6400 mg/L. Visible abnormalities (eg darker pigmentation, loss of equilibrium, disturbance in swimming behaviour or increased respiration rate) were observed in all fish at the nomi9nal 1600 mg/L concentration.
Results with reference substance (positive control):
Disodium heptachromate was used a reference material. The 24 h LC50 fulfilled the acceptance criterion of 200 - 400 mg/L.
Reported statistics and error estimates:
Not applicable

The test substance demonstrated low toxicity to fish in this acute study. The nominal 96 hour LC50 was found to be 2400 mg/L with a NOEC of 800 mg/L.

Validity criteria fulfilled:
yes
Conclusions:
96 hour LC50 was found to be 2400 mg/L
Executive summary:

The acute toxicity of Reaction mass of 2,2-bis(formyloxymethyl)propane-1,3-diyl diformate and formic acid / Formic acid tech 50% / 902-307-3 to Zebra fish was determined. The study was carried out according to OECD test guideline 203 - Acute Toxicity for Fish. In this study Zebra fish were exposed to the test material over a test period of 96 hours under semi-static conditions with test media renewal every 24 hours. The test material was dispersed in 5 L dilution water for the fish study.  New test solutions were prepared daily. Nominal exposure concentrations were 0 (control), 400, 800, 1600, 3200 and 6400 mg/L.  The results of the study were based on the nominal concentrations.

The 96 hour LC50 was observed to be 2400 mg/L and the corresponding NOEC, based on behavioural differences was observed to be 800 mg/L.

Description of key information

In an OECD 203 study the 96-hour LC50 was determined to be 2400 mg/L, and the NOEC 800 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
2 400 mg/L

Additional information

A semi-static GLP study to OECD 203 was carried out to determine the toxicity to fish. The test substance demonstrated low toxicity to fish in this acute study. The nominal 96 hour LC50 was found to be 2400 mg/L with a NOEC of 800 mg/L. The test substance should be considered non toxic to fish.