[(2-chlorophenyl)methylene]malononitrile

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference 1

Reference Type:

publication

Title:

2-Chlorobenzylmercapturic Acid, A Metabolite of the Riot Control Agent 2-Chlorobenzylidene Malononitrile (CS) in the Rat

Author:

E. C. Rietveld x, L. P. C. Delbressine, T. H. J. M.Waegemaekers, and F. Seutter-Berlage

Year:

1983

Bibliographic source:

Arch Toxicol (1983) 54:139-144

Reference 2

Reference Type:

review article or handbook

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

The Salmonella/microsome assay for mutagenicity was performed with salmonella Typhimurium TA 100, according to Ames et al. (1975).

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

[(2-chlorophenyl)methylene]malononitrile

EC Number:

220-278-9

EC Name:

[(2-chlorophenyl)methylene]malononitrile

Cas Number:

2698-41-1

Molecular formula:

C10H5ClN2

IUPAC Name:

[(2-chlorophenyl)methylene]malononitrile

Constituent 2

Reference substance name:

1,1,1,3,3,3-hexamethyldisilazane

EC Number:

213-668-5

EC Name:

1,1,1,3,3,3-hexamethyldisilazane

Cas Number:

999-97-3

Molecular formula:

C6H19NSi2

IUPAC Name:

1,1,1-trimethyl-N-(trimethylsilyl)silanamine

Constituent 3

Reference substance name:

Cab-0-Sil@ colloidal silica

IUPAC Name:

Cab-0-Sil@ colloidal silica

Method

Target gene:

Not specified

Species / strain

Species / strain / cell type:

S. typhimurium TA 100

Test concentrations with justification for top dose:

Test without metabolic activation : 5 concentrations : between 0 to 2000 µg/plate
Test with metabolic activation : several concentrations between 0 to 3500 µg/plate

Vehicle / solvent:

Not specified

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Remarks:

sodium azide : 1.0 µg or 2.5 µg/plate - benzoapyrène : 5.0 µg/plate

Positive control substance:

sodium azide

benzo(a)pyrene

Details on test system and experimental conditions:

The assays were performed without pre-incubation.

Rationale for test conditions:

Not specified

Evaluation criteria:

Not specified

Statistics:

Not specified

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

1000 µg/plate

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

With rat liver homogenate the bacteriotoxicity of CS was slightly stronger than without it.

Applicant's summary and conclusion

Conclusions:

No mutagenic effect was detected using CS either with or without rat liver homogenate (S-9 mix)

Executive summary:

In a study which methodology is comparable to OECD 471 (Ames test), no mutagenic effect was detected using CS either with or without rat liver homogenate (S-9 mix)