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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2018-04-02 to 2018-04-05
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Trihydrogen [29H,31H-phthalocyaninetrisulphonato(5-)-N29,N30,N31,N32]cuprate(3-), compound with N,N'-di(o-tolyl)guanidine (1:3)
EC Number:
277-086-3
EC Name:
Trihydrogen [29H,31H-phthalocyaninetrisulphonato(5-)-N29,N30,N31,N32]cuprate(3-), compound with N,N'-di(o-tolyl)guanidine (1:3)
Cas Number:
72928-60-0
Molecular formula:
C32H16-τN8Cu(SO3C15H18N3)τ
IUPAC Name:
Bis and tris and tetra [N-(2-methylanilino)-N’-(2-methylanilino) methaniminium][phthalocyaninesulfonato-κ4N29,N30,N31,N32]cuprate(II)
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
At test facility: 2017-04-19 to 2017-04-27: refrigerator (6 ± 2 °C)/ since 2017-04-27: room temperature, protected from light, in the tightly closed original container; since 2017-11-02 additionally in evacuated desiccator

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations:
* 0 mg/L of test item (control)
* 0.0500 mg/L of test item
* 0.158 mg/L of test item
* 0.500 mg/L of test item
* 1.58 mg/L of test item
* 5.00 mg/L of test item
* 15.8 mg/L of test item

- Sampling method: Sampling done in freshly prepared media at the start of exposure (0 hours) and in old media at the end of exposure (48 hours) without daphnids.
The control and the lower concentrated samples (0.0500 mg/L and 0.158 mg/L) were analyzed enriched factor 10.
The samples 0.500 mg/L and 1.58 mg/L were analyzed undiluted. The higher concentrated samples (5.00 mg/L and 15.8 mg/L) were diluted with dilution medium prior to analysis.

- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until the start of the analysis, if necessary. Prepared samples were stored in the autosampler at room temperature until analysis.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution with a nominal test item concentration of 15.8 mg/Lwas freshly prepared with dilution water one day prior to the start of the exposure (at day -1). This solution was stirred with a magnetic stirrer at approximately 1100 rpm for 24 hours at room temperature.
The four other concentrations: 0.0500 - 0.158 - 0.500 - 1.58 - 5.00 mg/L of test item were prepared by appropriate dilution of the stock solution.

- Controls: 4 replicates (without test item)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnid

- Strain/clone: STRAUSS

- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany

- Age of parental stock (mean and range, SD): less than 24 hours (at test initiation)

- Feeding during test: The daphnids were not fed during the study.


ACCLIMATION
- Acclimation period: No acclimation period as the daphnids were cultured under the same conditions as the test replicates.
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus provided at libitum
- Feeding frequency: at least 5 times per week
- Health during acclimation (any mortality observed): A COMPLETER

Study design

Test type:
static
Water media type:
other: medium Elendt M4 according to OECD 202, Annex 3 (2004)
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
18 - 22°C
pH:
8.2 ±0.8
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity


- Type : closed with watch glasses

- Material, size, headspace, fill volume: 250mL sterile glass Erlenmeyer flasks, filled at 20 mL.
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no renewal

- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates


OTHER TEST CONDITIONS
- Adjustment of pH: yes, for the culture medium
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: Diffuse light, maximum of 1500 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
A COMPLETER

VEHICLE CONTROL PERFORMED: no

RANGE-FINDING STUDY
- Test concentrations:
* 0 mg/l of test item (control)
* 0.0500 mg/l of test item
* 0.5000 mg/l of test item
* 5.00 mg/l of test item
- Results used to determine the conditions for the definitive study: Immobilization rates were used to determine the conditions for the definitive study
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 11.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CI 10.1 – 13.1
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
ca. 5.89 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CI 5.00 – 6.71
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
> 15.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 15.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 15.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 15.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: No mortality observed
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- ECx: EC50 at 2.00 mg/l
- 95% confidence limits: 1.00 - 4.00 mg/L
Reported statistics and error estimates:
EC10 and EC 50 are determined via the following the Sigmoidal dose-response (variable slope) following the equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the nominal concentrations of the test item, the 48-hour EC50 for Daphnia magna was 11.4 mg/L. No effects on Daphnia magna STRAUS were observed.
Executive summary:

In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined at the test facility according to OECD 202 (2004) from 2018-04-02 to 2018-04-05, with the definitive exposure phase from 2018-04-03 to 2018-04-05.

The study was conducted under static conditions over a period of 48 hours with the following nominal test item concentrations: 0.0500 - 0.158 - 0.500 - 1.58 - 5.00 - 15.8 mg/L of the test item.

The test item concentration was blue coloured in a concentration-related manner and visually clear throughout the exposure.

Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each test item concentration and the control.

The concentrations of the test item were analytically verified via HPLC-DAD in fresh media at the start (0 hours) and in old media at the end of the exposure (48 hours) in each test item concentration and in the control.

The measured concentrations of the test item were between 88 and 105% of the nominal test item concentration at 0 hours and between 93 and 103% at 48 hours, respectively.

The measured test item concentrations remained stable within ± 20% of the nominal limit concentration of the test item over the test period.

The validity criteria of the test guideline were fulfilled.

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