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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11/2017-05/2018
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium undec-10-enoate
EC Number:
222-264-8
EC Name:
Sodium undec-10-enoate
Cas Number:
3398-33-2
Molecular formula:
C11H20O2.Na
IUPAC Name:
sodium undec-10-enoate
Specific details on test material used for the study:
Purity: 99,3%

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Collect in Camposampiero ( PD) WT from Aerobic Aereation Re-circulation
Inoculum final concentration in every single bottle:
Suspended Solids ( 5.5.3.1) 27,1 mg/L
Microbiological Count (5.5.3.2.) UFC/L 6,0 x 10^7
Duration of test (contact time):
ca. 28 d
Initial test substance concentration
Initial conc.:
>= 10 - <= 20 mg/L
Based on:
TOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
A measured volume of inoculated mineral medium, containing a known concentration of the test
substance (10-20 mg DOC or TOC/l) as the nominal sole source of organic carbon is aerated by the
passage of carbon dioxide-free air at a controlled rate in the dark or in diffuse light. Degradation is
followed over 28 days by determining the carbon dioxide produced. The CO2 is trapped in barium
hydroxide and is measured by titration of the residual hydroxide or as inorganic carbon.
The amount of carbon dioxide produced from the test substance (corrected for that derived from the
blank inoculum) is expressed as a percentage of ThCO2. A substance is classified as ready
biodegradable when the amount of CO2 indicates a degradation greater than 60 %.
Reference substance
Reference substance:
benzoic acid, sodium salt
Remarks:
99.8%

Results and discussion

% Degradationopen allclose all
Key result
Parameter:
% degradation (CO2 evolution)
Value:
ca. 82.9
Sampling time:
28 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
> 60
Sampling time:
10 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Executive summary:

The biodegradability of this test sample was performed according to the OECD 301B : Co2 Evolution.

The test sample Biodegradation after 28 days > 60% ThCO2 (82.9%).

10-d windows : at the 10th test day from 10%, the biodegradability of the test item is superior to the 60%.

Therefore, Test sample results Ready Biodegradable according to the OECD 301B guidelines.