N-methylacetamide

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study, with acceptable restrictions (e.g. non-GLP, no analytical verification of test item concentrations).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-methylacetamide

EC Number:

201-182-6

EC Name:

N-methylacetamide

Cas Number:

79-16-3

Molecular formula:

C3H7NO

IUPAC Name:

N-methylacetamide

Details on test material:

- test substance: N-Methylacetamide
- purity: > 99 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- species: Pseudomonas putida, DSM 50026
- incubated at 24 °C (297 K +- 1 K), 150 rpm for 7+-1 h
- medium: AK-medium according to DIN 38412, Part 8 (draft)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test conditions

Details on test conditions:

- test vessel: 300 ml-Erlenmeyer flasks, 1 baffle
- liquid volume: 100 ml
- liquid volume: 10 ml (0.5 ml nutrient solution concentrate I (20x), 0.5 ml nutrient solution concentrate II (20x), 1 ml inoculum, 8 ml test item solution) - inoculum: 1 ml pre-culture (adjusted to standardized turbidity)
- test concentrations (mg/l): 0 (control), 312.5, 625, 1250, 2500, 5000, and 10000
- replicates: inoculated: 4 per concentration and control
- incubated at 20 °C (293 K)
- measurements: photometric determination at 436 nm
- pH determined at test start (0 h) and after 17 h

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

17 h

Dose descriptor:

other: TTC (Toxicity threshold concentration, comparable to EC10)

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Any other information on results incl. tables

RS-Freetext:
Concentration (mg/l)   Growth (% of control)
   0 (control)         100
  312.5                 90.74
  625                   90.84
 1250                  103.42
 2500                  103.63
 5000                  102.03
10000                  


pH at test start and test end:

test conc.      uninoculated   uninoculated
(mg/l)          (0 h)          (17 h)
   0 (control)  7.2            4.0
  312.5         7.2            4.4
  625           7.2            4.5
 1250           7.2            5.0
 2500           7.2            4.7
 5000           7.2            4.0
10000           7.1            3.6