Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

The key study is a reliable (Klimisch 1) guideline study measuring the immobilisation of Daphnia magna to the test substance, 9-Octadecenoic acid (Z)-, reaction products with diethylenetriamine, di-Me sulfate-quaternized. The test solutions were prepared as Water Accommodated Fractions (WAF). Due to the nature of the test substance (various constituents) the dissolved test item may have been one or several constituents of the test item and toxicity could not be attributed to a single component or mix of components. The results, therefore, are based on nominal loading rates. Under the conditions of the test, the 48 hour EL50 (immobility) was determined to be 0.89 mg/L, based on nominal loading rates. The NOELR was determined to be 0.63 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.89 mg/L

Additional information

The key study is a reliable (Klimisch 1) guideline study measuring the immobilisation of Daphnia magna to the test substance, 9-Octadecenoic acid (Z)-, reaction products with diethylenetriamine, di-Me sulfate-quaternized. The test solutions were prepared as Water Accommodated Fractions (WAF). Due to the nature of the test substance (various constituents) the dissolved test item may have been one or several constituents of the test item and toxicity could not be attributed to a single component or mix of components. The results, therefore, are based on nominal loading rates. Under the conditions of the test, the 48 hour EL50 (immobility) was determined to be 0.89 mg/L, based on nominal loading rates. The NOELR was determined to be 0.63 mg/L.

Two supporting studies are also presented for two read across substances. The first study (Klimisch 2) assessed the substance imidazolium compounds, 2-C17-unsatd.-alkyl-1-(2-C18-unsatd. amidoethyl)-4,5-dihydro-N-methyl, Me sul... / 931-745-8; oleic-acid based IQAC, DMS quaternised (two separate constituents). Immobilisation of Daphnia magna was assessed under static conditions, with a 48 hour EC50 of 0.065 mg/L based on a nominal concentration of active ingredient concentration. Measurement of exposure concentrations was not undertaken.

The other supporting study (Klimisch 2) was carried out on Fatty acids, C16-18 (even numbered) and C18 unsatd., reaction products with diethylene triamine, di (one constituent). A Water Accommodated Fraction method was used to prepare the test substance. The test was run as a static test. The test substance concentration was measured over time and the mean measured test item concentrations were calculated as the arithmetic means of the two geometric means of the concentrations measured at the start and end of the test medium renewal periods. The immobility of Daphnia magna with the 48-hour EC50 of 3.7 mg/L and were calculated by Weibull analysis using linear maximum likelihood regression. The 48-hour EC50 based on mean measured concentrations of the test item was 253 μg/L. The 48-hour NOEC based on immobilization was 0.32 mg/L based on loading rates and 15 μg/L based on mean measured concentrations.

Categories Display