C10-14-alkyl-1-(ethenyloxy)-

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 September to 12 October 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

yes

Remarks:

None that would compromise the result.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Kyoeisha; Bx 7080101
- Expiration date of the lot/batch: 01 Aug 2019
- Purity test date:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: stable
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: Test solutions were subject to active ingredient analysis using a JRF validated analytical method. For the main study, six replicates for the control and three replicates for treatment groups were prepared. 70 mL of test sample from each replicate were withdrawn and mixed for each group. Collected samples were centrifuged at 2000 rpm for 10 minutes to remove algal cells. The representative samples were divided into two equal portions. One portion was sent for test concentration analysis at 0 and 72 h, and the second portion was stored at -20 ± 5 ºC until report finalisation. Active ingredient concentration in test media was determined using the validated analytical method
- Sample storage conditions before analysis:

Vehicle:

yes

Remarks:

acetone

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Algae; Pseudokirchneriella subcapitata
- Strain: ATCC 22662
- Source (laboratory, culture collection): American collection, Virginia, USA.
- Age of inoculum (at test initiation):
- Method of cultivation: static

ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Hardness:

N/A

Test temperature:

21 to 24 °C, controlled at ± 2 °C

pH:

7.34 to 7.99

Dissolved oxygen:

N/A

Salinity:

N/A

Conductivity:

N/A

Nominal and measured concentrations:

Nominal; 0.0 (control), 10.7, 18.7, 32.7, 57.1, and 100.0 mg C10-C14 alkyl ether/L (geometric factor 1.75).
Measured @ 0h; 0.0, 1.5, 2.72, 4.99, 8.10, 16.09

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL conical flask.
- Type (delete if not applicable): open / closed no data
- Material, size, headspace, fill volume: 100 mL test solution.
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: 5605 cells/mL
- Control end cells density:
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):

GROWTH MEDIUM
- Standard medium used: yes/no
- Detailed composition if non-standard medium was used:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: 7.34 to 7.99
- Photoperiod:
- Light intensity and quality: 6710 to 6737 lux
- Salinity (for marine algae):

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Haemocytometer
- Chlorophyll measurement:
- Other:

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study A preliminary range finding study was conducted with the loading rates of 0.0 (control), 0.1, 1.0, 10.0, 50.0, and 100.0 mg C10-C14 alkyl ether/L. Two replicates were run for each concentration of test item and three replicates for the control.
- Test concentrations: 0, 10.7, 18.7, 32.7, 57.1 and 100.0 mg/L
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

13.282 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: CL 11.110 to 15.878 mg/L

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50: 1.15 mg/l
- Other:

Validity criteria fulfilled:

yes

Conclusions:

The ErC50 was found to be 13.282 mg/L (CL 11.110 to 15.878 mg/L)

Description of key information

The ErC50 was found to be 13.282 mg/L (CL 11.110 to 15.878 mg/L)

Key value for chemical safety assessment

EC50 for freshwater algae:

13.282 mg/L