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Administrative data

Endpoint:
melting point/freezing point
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 December 2017 - 08 February 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method A.1 (Melting / Freezing Temperature)
Version / remarks:
2016
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 102 (Melting point / Melting Range)
Version / remarks:
1995
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 830.7200 (Melting Point / Melting Range)
Version / remarks:
1998
Deviations:
no
GLP compliance:
yes
Type of method:
differential scanning calorimetry

Test material

Constituent 1
Chemical structure
Reference substance name:
Pregnenolone
EC Number:
205-647-4
EC Name:
Pregnenolone
Cas Number:
145-13-1
Molecular formula:
C21H32O2
IUPAC Name:
3-hydroxypregn-5-en-20-one
Test material form:
solid: particulate/powder
Details on test material:
- Physical appearance: white crystalline powder
- Storage conditions: at room temperature

Results and discussion

Melting / freezing pointopen allclose all
Key result
Melting / freezing pt.:
189.7 °C
Decomposition:
yes
Decomp. temp.:
325 °C
Key result
Melting / freezing pt.:
195.3 °C
Decomposition:
yes
Decomp. temp.:
325 °C

Any other information on results incl. tables

TGA EXPERIMENT (PRELIMINARY TEST)

Significant decrease of weight from 325°C upwards. 25% weight loss at 389°C and 70% weight loss at 424°C.

After the experiment, a light orange molten residue remained in the sample container (original colour: white). The change of the colour indicated reaction and/or decomposition of the test item.

DSC EXPERIMENT 1

Two endothermic peaks were observed between 175°C and 225°C followed by an endothermic effect starting at 325°C. The extrapolated onset temperature of the first peak was 189.718°C and the peak maximum of the second peak was 195.133°C. These endothermic effects were most likely obtained due to melting of the test item. The third endothermic effect was probably obtained due to reaction and/or decomposition of the test item. After the experiment, a light yellow molten residue remained in the sample container (original colour: white). The change of the colour indicated reaction and/or decomposition of the test item.

DSC EXPERIMENT 2

In order to investigate the melting peak, Experiment 2 was stopped directly after the second melting peak. The extrapolated onset temperature was of the first peak was 189.683°C and the peak maximum of the second peak was 195.480°C.  After the experiment, a white molten residue remained in the sample container. It demonstrated that melting was the reason for the endothermic effects.

DATA HANDLING

The first melting temperature was determined as the mean melting temperature of Experiment 1 (189.718°C) and Experiment 2 (189.683°C).

The second melting temperature was determined as the mean peak maximum of Experiment 1 (195.133°C) and Experiment 2 (195.480°C).

DSC curve recorded during heating: see Illustration

Applicant's summary and conclusion

Conclusions:
The melting temperatures of the substance were determined to be 189.7°C and 195.3°C.

Executive summary:

Differential Scanning Calorimetry (DSC) experiments were performed in a GLP study according to EC A.1, OECD 102 and OPPTS 830.7200.

Two melting temperatures were determined for the substance in duplicate obtaining mean values of 189.7°C and 195.3°C.

Reaction and/or decomposition of the substance started at 325°C.