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EC number: 205-271-0 | CAS number: 136-99-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-03-25 to 2014-05-2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to recognized international guidelines and GLP. No deviation from the guidelines was observed.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4,5-dihydro-2-undecyl-1H-imidazole-1-ethanol
- EC Number:
- 205-271-0
- EC Name:
- 4,5-dihydro-2-undecyl-1H-imidazole-1-ethanol
- Cas Number:
- 136-99-2
- Molecular formula:
- C16H32N2O
- IUPAC Name:
- 4,5-dihydro-2-undecyl-1H-imidazole-1-ethanol
- Test material form:
- solid: crystalline
- Details on test material:
- - State of aggregation:
- Particle size distribution:
- Mass median aerodynamic diameter (MMAD):
- Geometric standard deviation (GSD):
- Shape of particles:
- Surface area of particles:
- Crystal structure:
- Coating:
- Surface properties:
- Density:
- Moisture content:
- Residual solvent:
- Activation:
- Stabilisation:
- Other:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material:
- Expiration date of the lot/batch:
- Purity test date:
RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity:
- Specific activity:
- Locations of the label:
- Expiration date of radiochemical substance:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Store in a cool, dry and well ventilated place and away from foodstuffs. In darkness at room temperature.
- Stability under test conditions: Yes
- Solubility and stability of the test substance in the solvent/vehicle: Slighthly soluble in water 33.9 mg/L
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: Reacts with strong acids
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any): None
- Final dilution of a dissolved solid, stock liquid or gel: N/A
- Final preparation of a solid:
FORM AS APPLIED IN THE TEST (if different from that of starting material)
Remark: Environmental precautions: Do not allow to enter drains or waterways.
Do not discharge into the subsoil/soil
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: NIPPONT AUTOMOTIVE COATINGS Co. Ltd.
- Expiration date of the lot/batch: 5C12AA
- Purity test date: 96%STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: In darkness at room temperature
- Solubility and stability of the test substance in the solvent/vehicle:
SOLUBILITY IN WATER(20℃) ： Dispersible in water.
SOLVENT SOLUBILITY ： Soluble in MeOH,EtOH,Xylene,Toluene
- Stability under test conditions:
STABILITY ：Stable under normal temperature and pressure.
CONDITION TO AVOID ：May burn but does not ignite readily.Flammable poisonous
gases may accumulate in tanks and hopper cars.
May ignite combustible (wood,paper,oil,etc.).
INCOMPATABILITIES ：OXIDIZERS(STRONG)- Fire and explosion hazard.
HAZARDOUS ：Thermal decomposition products may include toxic oxides
DECOMPOSITION of carbon and nitrogen.
POLYMERIZATION ：Hazardous polymerization has not been reported to
occur under normal temperatures and pressures.
TREATMENT OF TEST MATERIAL PRIOR TO TESTING: According to the requirements of the Guideline
FORM AS APPLIED IN THE TEST (if different from that of starting material)Not different from the form applied of starting material.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Species/Origin: Aerobic activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage plant Rossdorf, Germany.
Conditioning: The aerobic activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated for one day. This suspension was used for the experiment. - Duration of test (contact time):
- >= 28 d
Initial test substance concentration
- Initial conc.:
- >= 103 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 11
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 83% after 14 days and after 28 days of incubation.
Any other information on results incl. tables
Biodegradation of Test Item
- Percentage Biodegradation: The test item contains nitrogen; therefore the evaluation of biodegradation has to be based onThODNH4 and ThODNO3 if the test item is biodegradable. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the 10-day window, describing the period between reaching at least 10% degradation and 60% degradation. This period should not exceed 10 days. The 10-day window started at day 17 based on ThODNH4 when the mean biodegradation was 10%. The end of the 10- day window was not within the experimental phase, the mean biodegradation at day 28 was 11%; the 10 day window criterion was not passed. Based on ThODNO3, the degradation was not greater than 10% within the experimental phase. The results are represented in Tables 2 - 4 and Figures 1 and 2.
Table 2. Cumulative Biochemical Oxygen Demand (mg O2/L) in Test Flasks during the Test Period of 28 Days
Time Flask No.
(days) 1 2 3 4 5 6 7
1 0 0 0 0 60 0 0
2 5 0 0 0 80 0 60
3 15 5 5 5 110 0 110
4 20 10 5 5 115 0 120
5 25 10 5 5 120 0 125
6 30 10 5 5 125 0 140
7 30 10 5 5 130 0 145
8 30 20 5 5 135 0 155
9 30 25 5 5 140 0 160
10 30 30 5 5 145 0 165
11 30 30 5 5 145 0 170
12 35 30 5 10 150 0 175
13 40 35 10 15 155 0 180
14 40 35 10 15 155 0 180
15 40 35 10 15 155 0 185
16 40 35 10 15 155 0 190
17 40 40 10 15 155 0 190
18 40 40 10 15 155 0 190
19 45 40 10 15 160 0 190
20 45 40 10 15 160 0 190
21 45 40 15 20 165 0 190
22 45 40 15 20 165 0 190
23 50 45 15 20 165 0 195
24 50 45 15 20 165 0 195
25 50 45 15 20 165 0 195
26 55 50 15 25 165 0 200
27 55 50 20 25 165 0 200
28 55 50 20 25 165 0 200
Flasks 1 and 2: test item
Flasks 3 and 4: inoculum control
Flask 5:reference (procedure control)
Flask 6: abiotic control
Flask 7: toxicity control
Table 3. Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control based on ThODNH4
Time Percentage Biodegradation1
(Days) Test item1 Sodium Benzoate2 Toxicity control1, 2
Flask 1 [%] Flask 2 [%] Flask 5 [%] Flask 7 [%]
1 0 0 35 0
2 2 0 47 14
3 4 0 62 24
4 6 2 64 26
5 7 2 67 27
6 9 2 70 30
7 9 2 73 32
8 9 6 76 34
9 9 7 79 35
10 9 9 82 36
11 9 9 82 37
12 10 8 83 38
13 10 8 83 38
14 10 8 83 38
15 10 8 83 39
16 10 8 83 40
17 10 10 83 40
18 10 10 83 40
19 12 10 86 40
20 12 10 86 40
21 10 8 86 39
22 10 8 86 39
23 12 10 86 40
24 12 10 86 40
25 12 10 86 40
26 13 11 85 41
27 12 10 83 40
28 12 10 83 40
1ThODNO3of test item: 3.099 mg O2/mg test item
2ThODNH4of sodium benzoate: 1.666 mg O2/mg reference item
Biodegradation of Reference Item Sodium Benzoate
- Percentage Biodegradation: The reference item sodium benzoate was sufficiently degraded to 83% after 14 days and after 28 days of incubation. The results are represented in Tables 2 -4 and Figures 1 and 2.
- Conclusion: The percentage biodegradation of the reference item confirms the suitability of the used aerobic activated sludge inoculum.
Biodegradation in the Toxicity Control
- Percentage Biodegradation: In the toxicity control containing both, the test item and reference item, 38% biodegradation was noted within 14 days and 40% biodegradation was determined after 28 days of incubation (after correction with the abiotic control). The results are represented in Tables 2 -4 and Figures 1 and 2.
- Conclusion: According to the test guidelines the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
Abiotic Control
Oxygen Demand: The oxygen demand in the abiotic control was 0 mg/L during the test duration, therefore no correction has to be made.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation rate of the test item did not reach 60% within the 10-day window or at the end of the test. Therefore, it is considered to be not readily biodegradable.
- Executive summary:
The test item was investigated for its ready biodegradability in a manometric respirometry test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The test item contains nitrogen; therefore the evaluation of biodegradation has to be based on ThODNH4 and ThODNO3 if the test item is biodegradable. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the 10-day window, describing the period between reaching at least 10% degradation and 60% degradation. This period should not exceed 10 days. The 10-day window started at day 17 based on ThODNH4 when the mean biodegradation was 10%. The end of the 10-day window was not within the experimental phase, the mean biodegradation at day 28 was 11%; the 10 day window criterion was not passed. Based on ThODNO3, the degradation was not greater than 10% within the experimental phase. Therefore, the test item is considered to be not readily biodegradable.
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