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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06/2013 to 12/2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Guideline:
other: The Guidelines for the Testing of Chemicals, State Environmental Protection Administration (SEPA) of China, 2004, No.201 "Algal Growth Inhibition Test".
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rewosoft TE 19L; Batch ST02697671

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

OTHER SPECIFICS:
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
stock solution with test culture medium, prepared by 24 h shaking at 23°C
Test organisms (species):
Chlorella vulgaris
Details on test organisms:
TEST ORGANISM
- Common name: Chlorella vulgaris
- Strain:
- Source (laboratory, culture collection): Shanghai Fisheries University
- Age of inoculum (at test initiation):
- Method of cultivation:

ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not): sam
- Any deformed or abnormal cells observed:
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
23 +- 2°C
pH:
8.0 +- 0.2
Nominal and measured concentrations:
Preliminary test: 0.1, 1, 10, 50, 100 mg/L nominal
Definitive test: 5.0, 8.5, 14.5, 24.56, 41.8 mg/L nominal, 5.43, 8.48, 13.41, 25.10, 45.11 mg/L measured
Details on test conditions:
TEST SYSTEM
- Test vessel: triangular flask
- fill volume: 300 mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density:
- Control end cells density: 2x10E4 cells/mL
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates):

GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: distilled water
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium: no
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod:light cycle: 14h/10h
- Light intensity and quality: 4400 - 8800 Lux


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 0.1, 1, 10, 50, 100 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
20.34 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
9.02 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Reported statistics and error estimates:
EC50 = 20.34 mg/L iwth 95 % confidence limit of 15.45 - 28.21 mg/L

           

 Concentration

mg/L

 0 h

[Algae cells/ml]

  24 h

[Algae cells/ml]

  48 h

[Algae cells/ml]

  72 h

[Algae cells/ml]

   72h-I (%)
 0  10000  50000  180000  800000  -
   10000  40000  170000  760000  
   10000  40000  160000  810000
5   10000  40000  170000  750000  
   10000  40000  140000  740000  1.08
 10000  40000 160000  770000  
 8.5  10000  30000  150000  660000  
   10000  40000  170000  630000  4.35
   10000  30000  130000  670000  
 14.5  10000  20000  50000  140000  
   10000  40000  80000  180000  36.66
   10000  30000  70000  160000  
 24.5  10000  20000  30000  60000  
   10000  20000  40000  70000  57.81
   10000  20000  30000  60000  
 41.8  10000  10000  10000  20000  
   10000  10000  10000  10000  89.42
   10000  10000  10000  20000  
Validity criteria fulfilled:
yes
Conclusions:
72h-ErC50 = 20.34 mg/L

Description of key information

72h-ErC50 = 20.34 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:
20.34 mg/L
EC10 or NOEC for freshwater algae:
9.02 mg/L

Additional information