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EC number: 824-263-3
CAS number: 2196165-14-5
Table 1. pH value thorough the test
Table 2. Growth rates
Table 3. % of inhibition of growth rate
Table 4. Biomass and % of inhibition of Biomass
The study of acute toxicity to aquatic plants of the substance was
For the determination of the inhibition of algal growth, the freshwater
aquatic plant Lemna minor has been used and following the operative
method described in the OECD Guideline 221 (2006).
The freshwater aquatic plant Lemna minor has been subject to the
substance at the test concentrations:
1; 3.2; 10; 32; 100; 320 y 100. The total duration of the trial
has been 7 days.
The result obtained in this test indicates that the tested substance has
an inhibition value of the growth rate EC50 (7d) equal to 1099 mg / L
(act.ingr.) after 7 days of testing and an inhibition value of the
biomass (yield) EC50 (7d) equal to 102 mg/L (act. ingr.)
There is one study conducted on the substance Direct Black RBK (CAS 2196165 -14 -5) according to the OECD Guideline 221 to determine the toxicity to aquatic plants Lemna minor. Based on the ECHA guidances, this test method is considered more suitable for testing toxicity of coloured test materials to aquatic plants, than the OECD guideline 201 to test the toxicity to aquatic algae.
The EC50 (7d) value 1099 mg/L active ingr. obtained in this study, shows that the tested substance is not harmful to this type of aquatic plant.
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