Reaction products of diazotised Benzenesulfonic acid, 2-amino-5-[[2-(sulfooxy)ethyl]sulfonyl]-, subsequently coupled with Urea, N-(3-aminophenyl)-, subsequently condensation with 1,3,5-Triazine, 2,4,6-trichloro-, further condensation with Benzenesulfonic acid, 3-amino- and 3-Pyridinecarboxylic acid, sodium salts

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Auguest 09, 2017 to October 11, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Dihua sewage treatment plant, Taiwan.

Duration of test (contact time):

29 d

Initial conc.:

20 mg/L

Based on:

TOC

Reference substance:

acetic acid, sodium salt

Remarks:

Sodium acetate

Parameter:

% degradation (CO2 evolution)

Value:

< 60

Sampling time:

29 d

Results with reference substance:

At Day 14 during the study, the biodegradation was 72.68 %

Table 1. The CO2 production of blank control group, test group, reference control group and toxicity control group

Time (Day)	Blank control group (mg)			Test group (mg)		Reference control group (mg)	Toxicity control group (mg)
	(1)	(2)	Mean	(1)	(2)
2	3.41	2.75	3.08	3.41	4.84	20.46	20.79
5	3.85	1.21	2.53	6.60	6.71	23.21	25.74
7	1.54	0.88	1.21	3.85	2.64	14.30	16.39
9	3.74	3.52	3.63	4.29	3.52	10.56	10.23
14	4.51	4.62	4.57	4.73	4.51	10.45	10.56
19	3.08	2.97	3.03	3.30	3.08	7.15	9.02
23	4.84	4.95	4.90	4.40	4.18	5.50	8.03
27	7.70	6.82	7.26	6.71	8.03	9.57	9.57
29	5.39	5.17	5.28	4.62	4.07	7.26	6.49

Table 2. The cumulative CO2 production and percentage biodegradation of blank control group, test group, reference control and toxicity control group

Time (Day)	Cumulative CO2 production (mg)					Percentage biodegradation (% ThCO2)
	Blank control group	Test group		Reference control group	Toxicity control group	Test group			Reference control group	Toxicity control group
	Mean	(1)	(2)			(1)	(2)	Mean
2	3.08	0.33	1.76	17.38	17.71	0.37	2.00	1.19	19.75	12.06
5	5.61	4.40	5.94	38.06	40.92	5.00	6.74	5.87	43.24	27.87
7	6.82	7.04	7.37	51.15	56.10	7.99	8.37	8.18	58.12	38.22
9	10.45	7.70	7.26	58.08	62.70	8.74	8.24	8.49	65.99	42.71
14	15.02	7.87	7.21	63.97	68.70	8.93	8.18	8.55	72.68	46.79
19	18.04	8.14	7.26	68.09	74.69	9.24	8.24	8.74	77.37	50.88
23	22.94	7.65	6.55	68.70	77.83	8.68	7.43	8.06	78.05	53.01
27	30.20	7.09	7.32	71.01	80.14	8.06	8.30	8.18	80.68	54.59
29	35.48	6.43	6.11	72.99	81.35	7.31	6.93	7.12	82.93	55.41

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

According to OECD 301B test method, CR SB07 was not be considered readily biodegradable.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-170700010001EN which is based on the SOP for the OECD 301B (SOPE-503) and OECD 301B (OECD, 1992). The results of this OECD 301B test for CR SB07 show that all validity criteria were met. CR SB07 can not be considered readily biodegradable under the test conditions and the percentage biodegradation of CR SB07 was less than 60 %.

Description of key information

Not readily biodegradable (OECD TG 301B).

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The total amount of evolved CO2 in the blank control group was 35.48 mg/1.2 L. The difference between replicates of test group was less than 20%. At Day 14 during the study, the biodegradation of the reference control article was72.68 % and the percent biodegradation in the toxicity control was 46.79 %. All test validity criteria were met. CR SB07 can not be considered readily biodegradable under the test conditions and the percentage biodegradation of CR SB07 was less than 60 %.